Abstract:
:Human CBS1 is a methionine sulfoxide reductase of type B (MSRB) as it specifically reduced Met-R-SO in peptides with dithiothreitol or the thioredoxin system as reductants. Mutation C169S in the active site completely abolished enzymatic activity, while mutation W110A only reduced activity and C105S had no effect. Like human MSRA, hCBS1 showed in vivo reducing activity coexpressed with the Drosophila ShC/B potassium channel in oocytes, by accelerating the overall inactivation time course. hCBS1-encoding mRNA is most abundant in muscle tissues, especially in the heart and thereby shows an expression pattern different to the human MSRA.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Jung S,Hansel A,Kasperczyk H,Hoshi T,Heinemann SHdoi
10.1016/s0014-5793(02)03171-xkeywords:
subject
Has Abstractpub_date
2002-09-11 00:00:00pages
91-4issue
1-3eissn
0014-5793issn
1873-3468pii
S001457930203171Xjournal_volume
527pub_type
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