Abstract:
:The extents to which different rate constants limit the steady-state rate of an isolated enzyme can be quantified as the control coefficients of those constants and elemental steps. We have found that the sum of the control coefficients of rate constants characterising unidirectional rates depleting a particular enzyme intermediate is equal to the concentration of that enzyme intermediate as a fraction of the total enzyme concentration. Together with simple measurements this powerful relation may be used (i) to estimate certain enzyme intermediate levels, in particular the free enzyme concentration, and (ii) to estimate the control coefficients of rate constants and steps.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kholodenko BN,Westerhoff HV,Brown GCdoi
10.1016/0014-5793(94)00617-2subject
Has Abstractpub_date
1994-07-25 00:00:00pages
131-4issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(94)00617-2journal_volume
349pub_type
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