Abstract:
:The odorant-induced accumulation of cAMP can be inhibited by antibodies directed against G alpha s/olf. In contrast, antibodies raised against G alpha i-subunits caused a strong enhancement of the odorant-induced cAMP accumulation. Western blotting and immunoelectron microscopy revealed the presence of both G alpha s/olf- and G alpha i-subunits in rat cilia preparations. The existence of both stimulatory and inhibitory odorant-induced regulation of adenylyl cyclase activity in olfactory cilia may indicate that an initial integration of different odorant stimuli begins at the level of primary reactions in the same effector enzyme.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sinnarajah S,Ezeh PI,Pathirana S,Moss AG,Morrison EE,Vodyanoy Vdoi
10.1016/s0014-5793(98)00368-8subject
Has Abstractpub_date
1998-04-24 00:00:00pages
377-80issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(98)00368-8journal_volume
426pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::A method was established for measuring molecule numbers of three different myosin heavy chain (MHC) mRNA isoforms in total RNA preparations. The quantification was based on a combination of primer-directed reverse transcriptase and polymerase chain reactions with 5'-digoxigenin-labeled oligonucleotides, using external...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80523-w
更新日期:1993-03-08 00:00:00
abstract::Several types of post-translational modifications contribute to the high level of tubulin heterogeneity in the brain. An important modification is glutamylation of the major brain-specific isotypes, such as class Ia/b of alpha-tubulin and classes II and III of beta-tubulin. Here we describe experiments to determine if...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01018-8
更新日期:1994-10-10 00:00:00
abstract::We investigated expression levels of Na(+),K(+)-ATPase alpha-isoforms and their ATPase activities in human colorectal cancer tissue and the accompanying normal mucosa. A decrease in expression of the alpha1-isoform protein was observed in all sampled cancer tissues compared with the normal mucosae. The level of ouabai...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(04)00292-3
更新日期:2004-04-09 00:00:00
abstract::Glycogen storage diseases type 1 (GSD 1) are a group of autosomal recessive disorders characterized by impairment of terminal steps of glycogenolysis and gluconeogenesis. Mutations of the glucose-6-phosphatase gene are responsible for the most frequent form of GSD 1, the subtype 1a, while mutations of the glucose-6-ph...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01248-x
更新日期:1999-10-08 00:00:00
abstract::F(0)F(1)-ATP synthase couples ATP synthesis/hydrolysis with transmembrane proton transport. The catalytic mechanism involves rotation of the gamma epsilon c(approximately 10)-subunits complex relative to the rest of the enzyme. In the absence of protonmotive force the enzyme is inactivated by the tight binding of MgAD...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2005.08.030
更新日期:2005-09-26 00:00:00
abstract::When isolated reaction centres of photosystem II are subjected to photoinhibitory illumination, a 41 kDa SDS-PAGE band is observed under all experimental conditions. The same band is also found, together with lower molecular weight fragments of the D1 protein, in whole thylakoids and in all PSII sub-particles investig...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81087-3
更新日期:1992-09-07 00:00:00
abstract::A 1000-bp fragment of Enterococcus hirae genomic DNA was amplified by the polymerase chain reaction method, using the oligonucleotide primers designed from amino acid sequences of both amino-terminal and a tryptic fragment of the Na(+)-ATPase alpha-subunit in this organism. DNA sequencing of this product revealed that...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80835-q
更新日期:1991-11-04 00:00:00
abstract::trans-3-Chloroacrylic acid dehalogenase (CaaD) catalyzes the hydrolytic dehalogenation of trans-3-haloacrylates to yield malonate semialdehyde by a mechanism utilizing βPro-1, αArg-8, αArg-11, and αGlu-52. These residues are implicated in a promiscuous hydratase activity where 2-oxo-3-pentynoate is processed to acetop...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.006
更新日期:2013-09-02 00:00:00
abstract::Intracellular vesicular transport involves targeting of vesicles to their correct destination and subsequent fusion with the target membrane. These processes employ unique compartment-specific proteins in combination with general components which act at several transport steps. This review will summarize recent progre...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(95)00594-y
更新日期:1995-08-01 00:00:00
abstract::Human monoclonal antibodies often display limited thermodynamic and colloidal stabilities. This behavior hinders their production, and places limitations on the development of novel formulation conditions and therapeutic applications. Antibodies are highly diverse molecules, with much of the sequence variation observe...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2013.11.029
更新日期:2014-01-21 00:00:00
abstract::HSP73, the constitutive form of heat-shock protein 70, has been implicated in the translocation of preproteins across the mitochondrial membranes, being required for maintaining mitochondrial preproteins in an import competent conformation. Here we report that highly purified mitochondrial outer membranes contain a pr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80649-f
更新日期:1993-10-18 00:00:00
abstract::C26, the C-terminal 26 residue peptide of serpin A1, significantly increased cell proliferation in cultures of hepatoma cells, but not in porcine kidney epithelial cells, human skin fibroblasts or keratinocytes. The mitogenic activity of C26 was preferentially inhibited with a protein kinase C (PKC) inhibitor, an anti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.09.035
更新日期:2004-10-22 00:00:00
abstract::Gap junctions and their connexin components are indispensable in mediating the cellular coordination required for tissue and organ homeostasis. The critical nature of their existence mandates a connection to disease while at the same time offering therapeutic potential. Therapeutic intervention may be offered through ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2014.02.047
更新日期:2014-04-17 00:00:00
abstract::E. coli GroEL chaperonin monomers, isolated after urea-induced dissociation of GroEL14, undergo cold denaturation below 5 degrees C. Above 5 degrees C, these monomers undergo MgATP-dependent self-assembly. We have demonstrated a conformational transition at 0 degree C induced by interaction of monomeric GroEL with ade...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81528-8
更新日期:1993-06-07 00:00:00
abstract::The present study was focused on the regulation of ecdysteroidogenesis in the Y-organ of Scylla serrata during molting cycle. A strong expression of molt-inhibiting hormone (MIH) and phosphorylation of ERK was predominantly observed in the postmolt and intermolt stages of Y-organs, whereas protein kinase C, steroidoge...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.09.029
更新日期:2007-11-13 00:00:00
abstract::Incubation of rat skeletal muscle mitochondria with the nitric oxide generator, S-nitrosoglutathione (GSNO) reversibly inhibited oxygen utilisation with all substrates tested. The visible absorption spectra of the inhibited mitochondria showed that cytochromes c+c1, b and a+a3 were reduced, indicating a block at the d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00424-2
更新日期:1994-05-23 00:00:00
abstract::Unstimulated human fibrosarcoma cells (HT1080) constitutively secrete matrix metalloproteinase 2 (MMP 2) as a proenzyme requiring proteolytic cleavage by membrane type-1 MMP (MT1 MMP) for activation. Physiological and pharmacological stimuli induce clustering of MT1 MMP/tissue inhibitor of MMP 2 "receptors", promoting...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.04.040
更新日期:2004-05-21 00:00:00
abstract::A monoclonal antibody (mAb) has been produced which reacts with human mitofilin, a mitochondrial inner membrane protein. This mAb immunocaptures its target protein in association with six other proteins, metaxins 1 and 2, SAM50, CHCHD3, CHCHD6 and DnaJC11, respectively. The first three are outer membrane proteins, CHC...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.06.052
更新日期:2007-07-24 00:00:00
abstract::The 3D structure of two unlabeled FK506 analogs, (R)- and (S)-[18-OH]ascomycin, when bound to [U-13C,15N]FKBP were determined by isotope-filtered 2D NMR experiments. The structures for the R and S isomers that bind tightly to FKBP but lack immunosuppressive activity are compared to each other and to the conformation o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81300-b
更新日期:1992-08-24 00:00:00
abstract::Oxaloacetate decarboxylase of Klebsiella pneumoniae was shown to contain between 0.6 and 1.0 mol zinc per mol enzyme in different preparations. The decarboxylase activity was completely abolished after 15 min incubation with 1 mM Hg(NO3)2 in phosphate buffer, while the activity decreased only 20% if the incubation was...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80165-d
更新日期:1992-03-23 00:00:00
abstract::We used transcriptomic (RNA-seq) analyses to determine whether patients suffering from all types and subtypes of mucopolysaccharidosis (MPS), a severe inherited metabolic disease, may be more susceptible to coronavirus disease 2019 (COVID-19). The expression levels of genes encoding proteins potentially involved in SA...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13908
更新日期:2020-10-01 00:00:00
abstract::Using antibodies recognizing the phosphorylation state of specific sites, phosphorylation states of tau were monitored in fetal rat primary cultured neurons. When cultured neurons were treated with okadaic acid (OA) or calyculin A (CalA) at concentrations sufficient to inhibit protein phosphatase 2A (PP2A), phosphoryl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01292-0
更新日期:1995-12-04 00:00:00
abstract::The effect of the inositol phospholipid-binding antibiotic neomycin was studied on high-affinity GTPase in human platelet membranes. At low concentrations (up to 1 mM), neomycin by itself stimulated a high-affinity GTPase. This GTPase stimulation was additive with that caused by the hormonal factors, prostaglandin E1 ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80795-6
更新日期:1988-02-29 00:00:00
abstract::Increasingly available genomic sequencing data are exploited to identify genes and variants contributing to diseases, particularly cancer. Traditionally, methods to find such variants have relied heavily on allele frequency and/or familial history, often neglecting to consider any mechanistic understanding of their fu...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12988
更新日期:2018-02-01 00:00:00
abstract::Using RT-PCR we distinguished mRNAs for all known G-protein coupled serotonin receptors expressed in various rat and porcine blood vessels. Nearly all vessels expressed 5HT1D beta, 5-HT2A, 5-HT2B, 5-HT4, and 5-HT7 receptor mRNA to different extents. New splice variants of the porcine 5-HT4 receptor were observed. Simi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00828-w
更新日期:1995-08-21 00:00:00
abstract::A recombinant expression system was developed to analyse sequence determinants involved in O-glycosylation of proteins in mycobacteria. By expressing peptide sequences corresponding to known glycosylation sites within a chimeric lipoprotein construct, amino acids flanking modified threonine residues were found to have...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01553-2
更新日期:2000-05-19 00:00:00
abstract::Farnesoid X receptor (FXR) is highly expressed in liver and intestine where it controls bile acid (BA), lipid and glucose homeostasis. Here we show that FXR is expressed and functional, as assessed by target gene expression analysis, in human islets and beta-cell lines. FXR is predominantly cytosolic-localized in the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.04.068
更新日期:2010-07-02 00:00:00
abstract::Silent information regulator 2 (Sir2) is an NAD(+)-dependent histone deacetylase that establishes repressive chromatin status and extends the life span of both budding yeast and the nematode worm Caenorhabditis elegans. There is growing evidence that its mammalian homologue Sir2alpha protects cells from stress-induced...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.09.051
更新日期:2006-10-30 00:00:00
abstract::We have previously shown that topoisomerase I (topo I) antagonist inhibited retrovirus replication. Since tyrphostins, synthetic compounds and protein tyrosine kinases (PTKs) blockers, inhibited topo I activity (manuscript in preparation) we examined their ability to inhibit Moloney murine leukemia virus (Mo-MuLV) rep...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80248-3
更新日期:1994-03-14 00:00:00
abstract::Biological functions of transcribed spacer regions in eucaryotic pre-rRNAs remain elusive so far. Utilization of the comparative approach to analyse their secondary structure has been impeded by the extensive sequence divergence observed among most of the specimens available to date. However, we have taken advantage o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81301-f
更新日期:1993-02-01 00:00:00