Conformation of two non-immunosuppressive FK506 analogs when bound to FKBP by isotope-filtered NMR.

abstract：:The possible in vivo interaction of the Nicotiana tabacum agglutinin (Nictaba) with endogenous glycoproteins was corroborated using a combination of confocal/electron microscopy of an EGFP-Nictaba fusion protein expressed in tobacco Bright Yellow-2 (BY-2) cells and biochemical analyses. In vitro binding studies demons...

journal_title：FEBS letters

authors： Lannoo N,Peumans WJ,Pamel EV,Alvarez R,Xiong TC,Hause G,Mazars C,Van Damme EJ

更新日期：2006-11-27 00:00:00

abstract：:Mammalian voltage-activated Shaker K+ channels associate with at least three cytoplasmic proteins: Kv beta 1, Kv beta 2 and Kv beta 3. These beta subunits contain variable N-termini, which can modulate the inactivation of Shaker alpha subunits, but are homologous throughout an aldo-keto reductase core. Human and ferre...

journal_title：FEBS letters

authors： McCormack K,McCormack T,Tanouye M,Rudy B,Stühmer W

更新日期：1995-08-14 00:00:00

abstract：:Ribosomes are ribozymes exerting substrate positioning and promoting substrate-mediated catalysis. Peptide-bonds are formed within a symmetrical region, thus suggesting that ribosomes evolved by gene-fusion. Remote interactions dominate substrate positioning at stereochemistry suitable for peptide-bond formation and e...

journal_title：FEBS letters

authors： Baram D,Yonath A

更新日期：2005-02-07 00:00:00

abstract：:Transcriptional responses to growth in high environmental calcium concentrations were characterized and compared between wild-type and mutant Arabidopsis plants containing a knockout mutation in the gene encoding a cyclic nucleotide-gated channel (CNGC2). We show that the transcriptional profile of cngc2 plants grown ...

journal_title：FEBS letters

authors： Chan CW,Wohlbach DJ,Rodesch MJ,Sussman MR

更新日期：2008-03-19 00:00:00

abstract：:During the course of large scale purification of the D1 dopamine receptor from rat brain, a protein of approximately 87,000 daltons (p87) was observed to copurify with the D1 receptor through four chromatographic steps. To characterize the nature of this protein, bovine and rat cDNA clones were isolated and sequenced....

journal_title：FEBS letters

authors： Gingrich JA,Andersen PH,Tiberi M,el Mestikawy S,Jorgensen PN,Fremeau RT Jr,Caron MG

更新日期：1992-11-09 00:00:00

abstract：:Signal transduction pathways control cellular responses to extrinsic and intrinsic signals. The yeast HOG (High Osmolarity Glycerol) response pathway mediates cellular adaptation to hyperosmotic stress. Pathway architecture as well as the flow of signal have been studied to a very high degree of detail. Recently, the ...

journal_title：FEBS letters

authors： Hohmann S

更新日期：2009-12-17 00:00:00

abstract：:In a subset of olfactory epithelium the odorant receptor guanylate cyclase, ONE-GC, is a central transduction component of the cyclic GMP signaling pathway. The odorant binds to the extracellular domain and activates its intracellular catalytic domain to generate the odorant second messenger, cyclic GMP. The present s...

journal_title：FEBS letters

authors： Duda T,Sharma RK

更新日期：2009-04-17 00:00:00

abstract：:This study describes the comparative analysis of two insect recombinant aminotransferases, Aedes aegypti 3-hydroxykynurenine (3-HK) transaminase/alanine glyoxylate aminotransferase (Ae-HKT/AGT) and Drosophila melanogaster serine pyruvate aminotransferase (Dm-Spat), which share 52% identity in their amino acid sequence...

journal_title：FEBS letters

authors： Han Q,Li J

更新日期：2002-09-11 00:00:00

abstract：:Activation of the formyl peptide chemoattractant receptor (FPCR) of phagocytic cells mobilizes intracellular calcium stores and affects the plasma membrane potential. Affinity crosslinking of FPCR has demonstrated a 60-80 kDa glycoprotein, with core peptide of 32 kDa. It is not known whether functional FPCR is this si...

journal_title：FEBS letters

authors： Murphy PM,Gallin EK,Tiffany HL,Malech HL

更新日期：1990-02-26 00:00:00

abstract：:Retinoic acid receptor-related orphan receptor-alpha (RORalpha) is a nuclear orphan receptor. Adenovirus-mediated overexpression of RORalpha1 and RORalpha4 suppressed tumor necrosis factor-alpha (TNF-alpha)-induced expression of vascular cell adhesion molecule-1 (VCAM-1) and intracellular adhesion molecule-1 (ICAM-1) ...

journal_title：FEBS letters

authors： Migita H,Satozawa N,Lin JH,Morser J,Kawai K

更新日期：2004-01-16 00:00:00

abstract：:1-Farnesylpyridinium (FPy), an analog of isoprenoid farnesol, initially induced morphological changes similar to those of typical apoptosis in human leukemia HL-60 cells but FPy-treated cells were characterized by the absolute absence of final apoptotic events such as fragmentation into apoptotic bodies. FPy-induced c...

journal_title：FEBS letters

authors： Hamada M,Nishio K,Doe M,Usuki Y,Tanaka T

更新日期：2002-03-13 00:00:00

abstract：:It had been shown previously by heterologous expression in Schizosaccharomyces pombe, that the two monosaccharide/H+ symporters HUP1 and HUP2 of Chlorella kessleri differ significantly concerning their substrate specificity: HUP1 transports predominantly D-glucose while HUP2 prefers D-galactose. Several chimeric trans...

journal_title：FEBS letters

authors： Will A,Tanner W

更新日期：1996-02-26 00:00:00

abstract：:A central step in xanthone biosynthesis is the formation of the C13 skeleton, i.e. an intermediate benzophenone. Biosynthesis of 2,3',4,6-tetrahydroxybenzophenone from m-hydroxybenzoyl-CoA and malonyl-CoA was shown in cell-free extracts from cultured cells of Centaurium erythraea. The enzyme catalyzing this reaction w...

journal_title：FEBS letters

authors： Beerhues L

更新日期：1996-04-01 00:00:00

abstract：:This study was performed to prove our hypothesis that the metabolite(s) of polycyclic aromatic hydrocarbons (PAHs) caused the activation or phosphorylation of p53 via DNA damage to suppress the liver X receptor (LXR)-mediated signal transductions as a probably more direct mechanism. We found that LXR-mediated trans-ac...

journal_title：FEBS letters

authors： Iwano S,Shibahara N,Saito T,Kamataki T

更新日期：2006-02-06 00:00:00

abstract：:A link between alterations in cholesterol homeostasis and Alzheimer's disease (AD) is nowadays widely accepted. However, the molecular mechanism/s underlying such link remain unclear. Numerous experimental evidences support the view that changes in neuronal membrane cholesterol levels and/or subcellular distribution d...

journal_title：FEBS letters

authors： Ledesma MD,Dotti CG

更新日期：2006-10-09 00:00:00

abstract：:Aah VI was isolated from the venom of the North African scorpion, Androctonus australis hector. It is the first glycosylated neurotoxin from scorpion venom to be described. It was not toxic to mice, when injected intracerebroventricularly at a dose of 1.2 microg per animal. However, it had typical activity in Blatella...

journal_title：FEBS letters

authors： Hassani O,Loew D,Van Dorsselaer A,Papandréou MJ,Sorokine O,Rochat H,Sampieri F,Mansuelle P

更新日期：1999-01-25 00:00:00

abstract：:Osteoblastic cells have been shown to be involved in osteoclast formation through cell to cell contacts. This study was designed to examine the possible function of vascular cell adhesion molecule 1 (VCAM-1) during osteoclastogenesis. As a source for stromal cells we used the recently established mouse bone marrow str...

journal_title：FEBS letters

authors： Feuerbach D,Feyen JH

更新日期：1997-01-27 00:00:00

abstract：:We have previously reported that synthetic dsRNA can activate p21 expression by targeting the p21 promoter, thereby suppressing the proliferation of human bladder cancer cells. As complementarity between dsRNA and its target sequences is necessary for RNA activation, miRNAs may also trigger p21 expression through the ...

journal_title：FEBS letters

authors： Wang C,Chen Z,Ge Q,Hu J,Li F,Hu J,Xu H,Ye Z,Li LC

更新日期：2014-12-20 00:00:00

abstract：:The positive strand RNA coronavirus, infectious bronchitis virus (IBV), induces a G2/M phase arrest and reduction in the G1 and G1/S phase transition regulator cyclin D1. Quantitative real-time RT-PCR and Western blot analysis demonstrated that cyclin D1 was reduced post-transcriptionally within infected cells indepen...

journal_title：FEBS letters

authors： Harrison SM,Dove BK,Rothwell L,Kaiser P,Tarpey I,Brooks G,Hiscox JA

更新日期：2007-04-03 00:00:00

abstract：:The uncoupler-induced inactivation of H+-ATPase in hepatoma 22a and mouse liver mitochondria has been studied. The dependence of this process on delta microH, and pH and ATP was established. The inactivated ATPase could be reactivated at alkaline pH values in the absence of ATP. These data indicate that the inactivati...

journal_title：FEBS letters

authors： Chernyak BV,Dukhovich VF,Khodjaev EYu

更新日期：1987-05-11 00:00:00

abstract：:A cell-free protein expression system was established that provides protein samples of adequate concentration and purity for direct NMR analysis. The Escherichia coli peptidyl-prolyl cis-trans isomerase PpiB was expressed in this system with dual amino acid-selective isotope labeling to identify the NMR signal from th...

journal_title：FEBS letters

authors： Guignard L,Ozawa K,Pursglove SE,Otting G,Dixon NE

更新日期：2002-07-31 00:00:00

abstract：:The roles of 2'-OH groups in the binding of mRNA to human ribosomes were studied using site-directed cross-linking. We found that both mRNA and mDNA analogues bearing a cross-linker can modify ribosomal proteins (rps) S3e and S2e at the mRNA entry site independently on tRNA presence, but only mRNA analogues were capab...

journal_title：FEBS letters

authors： Graifer D,Zhigailov A,Ven'yaminova A,Malygin A,Iskakov B,Karpova G

更新日期：2012-10-19 00:00:00

abstract：:Leaflets of Vicia faba with closed stomata or with opening stomata were freeze-dried. Excised guard-cell pairs were assayed individually under suboptimal conditions (pH 7.1 and subsaturating substrate) for phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) using quantitative histochemical procedures. L-Malate, 400 mi...

journal_title：FEBS letters

authors： Zhang SQ,Outlaw WH Jr,Chollet R

更新日期：1994-09-19 00:00:00

abstract：:We present a new water selective pulse sequence allowing rapid determination of exchange rates of labile protons on the millisecond time scale. Using diffusion measurements, exchange rates of resolved protons can be determined without prior knowledge of relaxation parameters in a short overnight experiment. The use of...

journal_title：FEBS letters

authors： Böckmann A,Guittet E

更新日期：1997-11-24 00:00:00

abstract：:We have analyzed the specificity of the actinomycin D-DNA interaction. The 'footprint' method has been used in this investigation. It is shown that: (i) The presence of dinucleotide GC or GG is required for binding of a single drug molecule. (ii) The strong binding sites are encoded by tetranucleotide XGCY; where X no...

journal_title：FEBS letters

authors： Scamrov AV,Beabealashvilli RS

更新日期：1983-11-28 00:00:00

abstract：:The purpose of the present study was to investigate whether 5-hydroxytryptamine (5-HT, serotonin) affects migration of vascular endothelial cells. 5-HT significantly enhanced migration of human aortic endothelial cells (HAECs), and this enhancement was completely inhibited by GR 55562, a 5-HT1 receptor antagonist, and...

journal_title：FEBS letters

authors： Matsusaka S,Wakabayashi I

更新日期：2005-12-19 00:00:00

abstract：:o-Aminophenol was found to be rapidly metabolized to a brown compound in the presence of purified human oxy- and methemoglobin, coupled with the oxidation and reduction of these hemoglobins by o-aminophenol. The final product of o-aminophenol was identified as 2-aminophenoxazine-3-one, by using spectrophotometry and H...

journal_title：FEBS letters

authors： Tomoda A,Yamaguchi J,Kojima H,Amemiya H,Yoneyama Y

更新日期：1986-02-03 00:00:00

abstract：:We previously isolated a Saccharomyces cerevisiae mutant (HsTnII), which displays 40% reduced chronological lifespan as compared to the wild type (WT). In this study, we found HsTnII cultures to be characterized by fragmented and dysfunctional mitochondria, and by increased initiation of apoptosis during chronological...

journal_title：FEBS letters

authors： Aerts AM,Zabrocki P,Govaert G,Mathys J,Carmona-Gutierrez D,Madeo F,Winderickx J,Cammue BP,Thevissen K

更新日期：2009-01-05 00:00:00

abstract：:Polyglutamine tract-binding protein 1 (PQBP1) is an intrinsically disordered protein abundantly expressed in the brain. Mutations in the PQBP1 gene are causative for X-linked mental retardation disorders. Here, we investigated the structure of the C-terminal segment within the context of full-length PQBP1. We produced...

journal_title：FEBS letters

authors： Nabeshima Y,Mizuguchi M,Kajiyama A,Okazawa H

更新日期：2014-12-20 00:00:00

abstract：:The C-terminal regions of Kv1 K+ channels show little conservation between isoforms except for the last four C-terminal residues, (E/L)TDV, which are well conserved from Drosophila to man. Deletions of the 4, 16, and 57 C-terminal residues of the human Kv1.5 channel did not affect whole cell current amplitude, midpoin...

journal_title：FEBS letters

authors： Uebele VN,Yeola SW,Snyders DJ,Tamkun MM

更新日期：1994-02-28 00:00:00