Alternative splicing of the human Shaker K+ channel beta 1 gene and functional expression of the beta 2 gene product.

abstract：:Irreversible conversion of the purified zinc-containing 7Fe ferredoxin from the thermoacidophilic archaeon Sulfolobus sp. strain 7 was found to occur under aerobic conditions at pH 5.0 and at 4 degrees C. This process accompanied a substantial increase of the electron paramagnetic resonance signal attributed to a [3Fe...

journal_title：FEBS letters

authors： Iwasaki T,Oshima T

更新日期：1997-11-10 00:00:00

abstract：:The 4-chlorobenzoyl-CoA dehalogenase catalyzes the hydrolytic dechlorination of 4-chlorobenzoyl-CoA via a two-step mechanism, namely nucleophilic aromatic substitution and ester hydrolysis. The mutation of an active-site Histidine residue has been shown to reduce the catalytic activity in both the substitution and sub...

journal_title：FEBS letters

authors： Xu D,Guo H

更新日期：2005-08-15 00:00:00

abstract：:Hydrogenase gene from Clostridium butyricum was cloned in Escherichia coli HK16 (Hyd-) using pBR322 and PstI. The plasmid, pCBH1, containing hydrogenase gene was 7.3 MDa and pCBH1 had 5 PstI-DNA fragments (3.9, 2.6, 0.7, 0.03-0.04, less than 0.02 MDa, respectively). The hydrogenase activity of HK16 (pCBH1) was about 3...

journal_title：FEBS letters

authors： Karube I,Urano N,Yamada T,Hirochika H,Sakaguchi K

更新日期：1983-07-11 00:00:00

abstract：:Three novel neuropeptides, isolated from ganglia of the African giant snail, Achatina fulica, were named WWamide-1, -2 and -3. These substances were biologically active heptapeptide amides with a Trp residue at both the N- and C-termini. WWamide-1, which displayed an inhibitory activity on a central neuron of the snai...

journal_title：FEBS letters

authors： Minakata H,Ikeda T,Muneoka Y,Kobayashi M,Nomoto K

更新日期：1993-05-24 00:00:00

abstract：:Peptic digestion of bovine hemoglobin yields a fragment with antibacterial activity. This peptide was purified to homogeneity by a two-step procedure including anion exchange chromatography and preparative reversed-phase HPLC. Mass determination and fragmentation indicated that this peptide corresponded to the 1-23 fr...

journal_title：FEBS letters

authors： Froidevaux R,Krier F,Nedjar-Arroume N,Vercaigne-Marko D,Kosciarz E,Ruckebusch C,Dhulster P,Guillochon D

更新日期：2001-02-23 00:00:00

abstract：:Analysis of 13 high-resolution protein X-ray crystal structures shows that 1204 (24%) of all the 4974 hydrogen bonds are of the bifurcated three-center type with the donor X-H opposing two acceptors A1, A2. They occur systematically in alpha-helices where 90% of the hydrogen bonds are of this type; the major component...

journal_title：FEBS letters

authors： Preissner R,Egner U,Saenger W

更新日期：1991-08-19 00:00:00

abstract：:cDNAs encoding the isoform I alpha of the cGMP-dependent protein kinase were isolated from a bovine trachea smooth muscle cDNA library constructed in lambda gt10. The deduced protein sequence is identical with the protein sequence obtained by Edman degradation of the bovine lung enzyme [(1984) Biochemistry 23, 4207-42...

journal_title：FEBS letters

authors： Wernet W,Flockerzi V,Hofmann F

更新日期：1989-07-17 00:00:00

abstract：:Tau is reversibly hyperphosphorylated in the mouse brain by starvation or cold water swimming. Here, we report tau phosphorylation in the hippocampus of normal mouse after ether anesthesia, known to trigger typical stress reactions. Robust phosphorylation of tau was observed immediately and 10min after ether vapor exp...

journal_title：FEBS letters

authors： Ikeda Y,Ishiguro K,Fujita SC

更新日期：2007-03-06 00:00:00

abstract：:The amino acid sequence of the eclosion hormone from the tobacco hornworm Manduca sexta has been determined, using less than 500 pmol of protein and microanalytical techniques. The protein contains 62 amino acid residues and has a molecular mass of 6813 Da. The amino-terminal sequence is similar to that of a 13-residu...

journal_title：FEBS letters

authors： Marti T,Takio K,Walsh KA,Terzi G,Truman JW

更新日期：1987-07-27 00:00:00

abstract：:Two alpha-type CK2-activated PKAs (CK2-aPKAIalpha and CK2-aPKAIIalpha) were biochemically characterized in vitro using GST-HBV core fusion protein (GST-Hcore) and GST-Hcore157B as phosphate acceptors. It was found that (i), in the absence of cAMP, these two CK2-aPKAs phosphorylated both Ser-170 and Ser-178 on GST-Hcor...

journal_title：FEBS letters

authors： Enomoto M,Sawano Y,Kosuge S,Yamano Y,Kuroki K,Ohtsuki K

更新日期：2006-02-06 00:00:00

abstract：:Recently the identity of the peptidyl-prolyl cis-trans isomerase (PPIase), which accelerates the cis/trans isomerization of prolyl peptide bonds and cyclophilin, the binding protein for the immunosuppressive drug Cyclosporin A (CsA), was discovered. The PPIase catalysis toward the substrate Suc-Ala-Phe-Pro-Phe-pNA has...

journal_title：FEBS letters

authors： Hübner D,Drakenberg T,Forsén S,Fischer G

更新日期：1991-06-17 00:00:00

abstract：:Linkage between the high-capacity Ca2+-binding protein calsequestrin and the ryanodine receptor is proposed to be essential for proper Ca2+-release during skeletal muscle excitation-contraction coupling. However, no direct biochemical evidence exists showing a connection between these high-molecular-mass complexes in ...

journal_title：FEBS letters

authors： Murray BE,Ohlendieck K

更新日期：1998-06-16 00:00:00

abstract：:We have recently developed a method to produce native human proinsulin using a bacterial expression system. A proinsulin fusion protein was recovered from inclusion bodies and cleaved using cyanogen bromide. The released proinsulin polypeptide was S-sulfonated and purified by anion exchange chromatography. Following r...

journal_title：FEBS letters

authors： Cowley DJ,Mackin RB

更新日期：1997-02-03 00:00:00

abstract：:The amino acid sequence of EcoRV DNA methyltransferase which methylates the amino group of the 5'-adenine residue of the target sequence GATATC has been found to be closely related to that of three other adenine methyltransferases, DpnII, dam and damT4, the target sequence of which is GATC. Despite large differences o...

journal_title：FEBS letters

authors： Lauster R,Kriebardis A,Guschlbauer W

更新日期：1987-08-10 00:00:00

abstract：:The gene for the extracellular ribonuclease of B. pumilus KMM62 (RNase Bp) has been cloned and sequenced. The structural gene for this enzyme is similar to those of the extracellular ribonucleases of B. intermedius 7P (binase) and B. amyloliquefaciens H2 (barnase), as are the regulatory regions of binase and RNase Bp....

journal_title：FEBS letters

authors： Znamenskaya LV,Gabdrakhmanova LA,Chernokalskaya EB,Leshchinskaya IB,Hartley RW

更新日期：1995-01-02 00:00:00

abstract：:The ATP-binding cassette (ABC) transporters constitute one of the largest families of proteins in evolution. The ATM1 gene of the yeast Saccharomyces cerevisiae encodes an ABC protein, which is localized to the mitochondrial inner membrane. A deletion of ATM1 results in the accumulation of up to a 30-fold excess of mi...

journal_title：FEBS letters

authors： Chloupková M,Reaves SK,LeBard LM,Koeller DM

更新日期：2004-07-02 00:00:00

abstract：:Regulators of G protein signaling RGS4 and RGS7 accelerate the kinetics of K(+) channels (GIRKs) in the Xenopus oocyte system. Here, via quantitative analysis of RGS expression, we reveal biphasic effects of RGSs on GIRK regulation. At low concentrations, RGS4 inhibited basal GIRK activity, but stimulated it at high c...

journal_title：FEBS letters

authors： Keren-Raifman T,Bera AK,Zveig D,Peleg S,Witherow DS,Slepak VZ,Dascal N

更新日期：2001-03-09 00:00:00

abstract：:α-Helical membrane-active antimicrobial peptides (AMPs) are known to act via a range of mechanisms, including the formation of barrel-stave and toroidal pores and the micellisation of the membrane (carpet mechanism). Different mechanisms imply that the peptides adopt different 3D structures when bound at the water-mem...

journal_title：FEBS letters

authors： van den Bergen G,Stroet M,Caron B,Poger D,Mark AE

更新日期：2020-03-01 00:00:00

abstract：:The thermophilic cyanobacterial strain, PCC7518, originally identified as 'Mastigocladus laminosus HTF' does not show branchings or heterocysts. The absence of branchings supports the later assignment to the genus Chlorogloeopsis. The absence of heterocysts may be the result of a mutation because heterocysts were obse...

journal_title：FEBS letters

authors： Wilmotte A,Van der Auwera G,De Wachter R

更新日期：1993-02-08 00:00:00

abstract：:Both acute myeloid leukemia 1 and c-Fos are regulatory factors of hematopoietic cell differentiation. We identified that the c-fos promoter contains an acute myeloid leukemia 1 binding site at nucleotide positions -6-+14. c-fos promoter activity was induced by transient overexpression of acute myeloid leukemia 1 in Ju...

journal_title：FEBS letters

authors： Hwang ES,Hong JH,Bae SC,Ito Y,Lee SK

更新日期：1999-03-05 00:00:00

abstract：:Calcium release from internal stores of Entamoeba histolytica, a parasitic protozoan, was observed by measuring fluorescence of Fura-2. Emptying of inositol(1,4,5)trisphosphate (Ins(1,4,5)P3)-sensitive calcium pools in permeabilized E. histolytica did not significantly affect subsequent calcium release by inositol(1,3...

journal_title：FEBS letters

authors： Raha S,Giri B,Bhattacharyya B,Biswas BB

更新日期：1995-04-10 00:00:00

abstract：:The present study provides the first evidence that the abundance of catalytic alpha1-subunit of Na,K-ATPase increases in the course of T cell blast transformation. Immunodepressant cyclosporin A at anti-proliferative doses diminished the induction of alpha1 protein in activated lymphocytes. Furthermore, in competent T...

journal_title：FEBS letters

authors： Marakhova I,Karitskaya I,Aksenov N,Zenin V,Vinogradova T

更新日期：2005-05-23 00:00:00

abstract：:Vacuolar ATPases (V1V0 -ATPases) function in proton translocation across lipid membranes of subcellular compartments. We have used antibody labeling and electron microscopy to define the position of subunit C in the vacuolar ATPase from yeast. The data show that subunit C is binding at the interface of the ATPase and ...

journal_title：FEBS letters

authors： Zhang Z,Inoue T,Forgac M,Wilkens S

更新日期：2006-04-03 00:00:00

abstract：:The existence of the internal promoter Pi in the 16 S/23 S intergenic spacers of the rRNA operons of an eubacterium Escherichia coli and archaebacterium Halobacterium halobium is proposed. The possible functional significance of these promoters is discussed. ...

journal_title：FEBS letters

authors： Mankin AS,Skripkin EA,Kagramanova VK

更新日期：1987-07-27 00:00:00

abstract：:The guanine nucleotide exchange reaction catalyzed by elongation factor Ts is proposed to arise from the intrusion of the side chains of D80 and F81 near the Mg2+ binding site in EF-Tu. D80A and F81A mutants of E. coli EF-Ts were 2-3-fold less active in promoting GDP exchange with E. coli EF-Tu while the D80AF81A muta...

journal_title：FEBS letters

authors： Zhang Y,Li X,Spremulli LL

更新日期：1996-08-12 00:00:00

abstract：:Two phosphoenolpyruvate carboxylase (PEPC) kinase genes (PPCk1 and PPCk2) are present in the Arabidopsis genome; only PPCk1 is expressed in rosette leaves. Homozygous lines of two independent PPCk1 T-DNA-insertional mutants showed very little (dln1), or no (csi8) light-induced PEPC phosphorylation and a clear retard i...

journal_title：FEBS letters

authors： Meimoun P,Gousset-Dupont A,Lebouteiller B,Ambard-Bretteville F,Besin E,Lelarge C,Mauve C,Hodges M,Vidal J

更新日期：2009-05-19 00:00:00

abstract：:The transcription factor Pdr1p recognizes Pdr1p/Pdr3p-response element (PDRE) to activate genes involved in multidrug resistance of the budding yeast. To identify novel targets of Pdr1p, we compared transcriptomes among the yeast cells bearing wild, disrupted and gain-of-function alleles of PDR1 using a high-throughpu...

journal_title：FEBS letters

authors： Miura F,Yada T,Nakai K,Sakaki Y,Ito T

更新日期：2001-09-07 00:00:00

abstract：:Interaction of the water oxidising manganese complex of photosystem II with the aqueous environment has been investigated using electron paramagnetic resonance spectroscopy and electron spin echo envelope modulation spectroscopy to detect interaction of [2H]methanol with the complex in the S2 state. The experiments sh...

journal_title：FEBS letters

authors： Evans MC,Gourovskaya K,Nugent JH

更新日期：1999-05-07 00:00:00

abstract：:The transmembrane protein MIG-13 is a key regulator required for anterior migration of neural cells in Caenorhabditis elegans, but the signaling mechanisms involved remain unknown. Here, we isolated a suppressor mutation in the unc-71/adm-1 gene, which rescued the AVM neuron migration defect in mig-13 mutants. Genetic...

journal_title：FEBS letters

authors： Masuda H,Nakamura K,Takata N,Itoh B,Hirose T,Moribe H,Mekada E,Okada M

更新日期：2012-03-23 00:00:00

abstract：:The large rep gene products, Rep78 and Rep68, of adeno-associated virus (AAV) are pleiotropic effector proteins which are required for AAV DNA replication and the trans-regulation of AAV gene expression. Apart from these essential functions prerequisite for the life cycle of AAV, these rep products are able to inhibit...

journal_title：FEBS letters

authors： Batchu RB,Hermonat PL

更新日期：1995-07-03 00:00:00