Abstract:
:Hydrogenase gene from Clostridium butyricum was cloned in Escherichia coli HK16 (Hyd-) using pBR322 and PstI. The plasmid, pCBH1, containing hydrogenase gene was 7.3 MDa and pCBH1 had 5 PstI-DNA fragments (3.9, 2.6, 0.7, 0.03-0.04, less than 0.02 MDa, respectively). The hydrogenase activity of HK16 (pCBH1) was about 3.1-3.5-times as high as those of the present strains, such as C.butyricum and E.coli C600 (Hyd+).
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Karube I,Urano N,Yamada T,Hirochika H,Sakaguchi Kdoi
10.1016/0014-5793(83)80689-9subject
Has Abstractpub_date
1983-07-11 00:00:00pages
119-22issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(83)80689-9journal_volume
158pub_type
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