Abstract:
:Vav functions as a specific GDP/GTP nucleotide exchange factor which is regulated by tyrosine phosphorylation in the hematopoietic system. Loss of the amino-terminus sequences of Vav was sufficient to control its transforming potential and its function in T cells. We report here the identification of the hematopoietic GDP dissociation inhibitor protein, Ly-GDI, as a protein that interacts with the amino-terminus of Vav. Further analysis confirmed that Vav and Ly-GDI interact both in in vitro and in in vivo assays. This association is maximal only when the amino region of Vav is intact and requires an intact carboxy-terminus of Ly-GDI. The interaction between Vav and Ly-GDI is not dependent on the tyrosine phosphorylation status of Vav. In addition, Rho-GDI, the highly homologous protein to Ly-GDI, associates with Vav as well. The contribution of the interaction between Vav and GDIs, proteins that are involved in the GDP/GTP exchange processes, to the biological function of Vav is further discussed.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Groysman M,Russek CS,Katzav Sdoi
10.1016/s0014-5793(00)01121-2keywords:
subject
Has Abstractpub_date
2000-02-04 00:00:00pages
75-80issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(00)01121-2journal_volume
467pub_type
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