Abstract:
:The gene for titin, a 4MDa myofibrillar protein, was analysed in golden hamster DNAs from different sources, using human cDNA probes and PCR. In the DNA from the BHK-21-Bi subline of baby hamster kidney cells, extended sequences coding for Z-line associated domains were missing, indicating a deletion that renders titin non-functional. These sequences were present in the original BHK-21 line and in hamster DNAs. Our finding shows that, due to the absence of selective pressure on a gene's function, genomic deterioration can occur in a permanent cell line and can lead to a loss of overlapping DNA stretches in both autosomes.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Jäckel M,Witt C,Antonova O,Curdt I,Labeit S,Jockusch Hdoi
10.1016/s0014-5793(97)00381-5subject
Has Abstractpub_date
1997-05-12 00:00:00pages
21-4issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(97)00381-5journal_volume
408pub_type
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