Abstract:
:Depending on experimental conditions we have found that photo-inhibitory treatment of photosystem II (PSII) core complexes, isolated from wheat, can generate two fragments of about 23-24 kDa that contain either the C-terminal or N-terminal regions of the D1-protein. A 24 kDa C-terminal fragment appears when the water splitting reaction is not functional and an electron acceptor is present. This 'donor'-side inhibition also generates an N-terminal fragment of about 10 kDa and is suggested to be due to the cleavage of a peptide bond in the region connecting transmembrane segments I and II of the D1-protein. In contrast, an N-terminal 23 kDa D1-protein fragment is detected when the water splitting reactions of the isolated complex are active, and occurs in the absence of an added electron acceptor. This 'acceptor'-side photo-inhibition also generates a C-terminal fragment of about 10 kDa.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
De Las Rivas J,Andersson B,Barber Jdoi
10.1016/0014-5793(92)80250-kkeywords:
subject
Has Abstractpub_date
1992-04-27 00:00:00pages
246-52issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(92)80250-Kjournal_volume
301pub_type
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