Mia40, a novel factor for protein import into the intermembrane space of mitochondria is able to bind metal ions.

abstract：:The primary structure of a novel subunit of the mouse NMDA (N-methyl-D-aspartate) receptor channel, designated epsilon 4, has been revealed by cloning and sequencing the cDNA. The epsilon 4 subunit shares high amino acid sequence identity with the epsilon 1, epsilon 2 and epsilon 3 subunits of the mouse NMDA receptor ...

journal_title：FEBS letters

authors： Ikeda K,Nagasawa M,Mori H,Araki K,Sakimura K,Watanabe M,Inoue Y,Mishina M

更新日期：1992-11-16 00:00:00

abstract：:Alterations in both calcitonin (CT) secretion and plasma calcium were recently described in adult obese Zucker rats. We have investigated the CT biosynthetic activity of thyroid glands in 30-day-old obese Zucker rats (fa/fa), and their controls (Lean). Plasma calcium level was significantly increased (+0.6 mg/dl) in o...

journal_title：FEBS letters

authors： Segond N,Jullienne A,Tahri EH,Garel JM

更新日期：1984-08-20 00:00:00

abstract：:By using monoiodinated radioligands of both intact neuropeptide Y (NPY) and of a long C-terminal fragment, NPY13-36, two subtypes of binding sites, which differ in affinity and specificity, have been characterized. The Y1 type of binding site, characterized on a human neuroblastoma cell line, MC-IXC, and a rat pheochr...

journal_title：FEBS letters

authors： Sheikh SP,Håkanson R,Schwartz TW

更新日期：1989-03-13 00:00:00

abstract：:In response to growth factors, quiescent fibroblast mutants lacking Na+/H+ exchange activity fail to elevate their cytoplasmic pH (pHi) and to reinitiate DNA synthesis at neutral and acidic pHo. A pHi threshold of approximately 7.2 exists, below which growth factors cannot set in motion the Go to S phase transition. R...

journal_title：FEBS letters

authors： Pouysségur J,Franchi A,L'Allemain G,Paris S

更新日期：1985-10-07 00:00:00

abstract：:We have determined the first de novo position of the secondary quinone QB in the Rhodobacter sphaeroides reaction center (RC) using phases derived by the single wavelength anomalous dispersion method from crystals with selenomethionine substitution. We found that in frozen RC crystals, QB occupies primarily the proxim...

journal_title：FEBS letters

authors： Pokkuluri PR,Laible PD,Crawford AE,Mayfield JF,Yousef MA,Ginell SL,Hanson DK,Schiffer M

更新日期：2004-07-16 00:00:00

abstract：:The role of tRNA in translating the genetic code has received considerable attention over the last 50 years, and we now know in great detail how particular amino acids are specifically selected and brought to the ribosome in response to the corresponding mRNA codon. Over the same period, it has also become increasingl...

journal_title：FEBS letters

authors： Banerjee R,Chen S,Dare K,Gilreath M,Praetorius-Ibba M,Raina M,Reynolds NM,Rogers T,Roy H,Yadavalli SS,Ibba M

更新日期：2010-01-21 00:00:00

abstract：:Treatment of rat thymus cell suspensions with dexamethasone resulted in inhibition of engaged RNA polymerase A, without significant change in free pool activity. Studies with the re-initiation inhibitor, rifamycin AF/0-13, and measurements of numbers of RNA polymerase A molecules and of elongation rates showed that th...

journal_title：FEBS letters

authors： Dembinski TC

更新日期：1984-07-23 00:00:00

abstract：:Epidermal growth factor (EGF) induces transformed phenotypes in EGF receptor-overexpressing NIH3T3 (ER12) cells. Tyrosine kinase inhibitors such as erbstatin and its stable analogue methyl 2,5-dihydroxycinnamate inhibited the EGF-induced phenotypic changes in these cells; while 5'-O-methylerbstatin, an inactive analog...

journal_title：FEBS letters

authors： Umezawa K,Sugata D,Yamashita K,Johtoh N,Shibuya M

更新日期：1992-12-21 00:00:00

abstract：:Using radiolabeled specific cDNA glutamine synthetase mRNA could be detected by in situ hybridization exclusively within those few perivenous hepatocytes which stained immunocytochemically for glutamine synthetase. This localization of glutamine synthetase mRNA was recently reported by Moorman et al. [(1988) J. Histoc...

journal_title：FEBS letters

authors： Gebhardt R,Ebert A,Bauer G

更新日期：1988-12-05 00:00:00

abstract：:The mediator complex in the RNA polymerase II holoenzyme is known to be involved in transcriptional activation. The role of the essential mediator component Srb7p has been difficult to investigate, since no conditional lethal allele has been available to date. While the expression of Srb7p under the control of a repre...

journal_title：FEBS letters

authors： Gromöller A,Lehming N

更新日期：2000-10-27 00:00:00

abstract：:The complexity of the proteasome gene family in higher plants was investigated by identification and sequencing cDNA clones from the Arabidopsis thaliana database showing homologies to 20S proteasome subunits. We identified plant counterparts for each of the 14 proteasomal subunit subfamilies. Moreover, several of the...

journal_title：FEBS letters

authors： Parmentier Y,Bouchez D,Fleck J,Genschik P

更新日期：1997-10-27 00:00:00

abstract：:The phytocannabinoid Delta(9)-Tetrahydrocannabinol (Delta(9)-THC), the main psychoactive cannabinoid in cannabis, activates a number of signalling cascades including p53. This study examines the role of Delta(9)-THC in regulating the p53 post-translational modifier proteins, Murine double minute (Mdm2) and Small Ubqui...

journal_title：FEBS letters

authors： Gowran A,Murphy CE,Campbell VA

更新日期：2009-11-03 00:00:00

abstract：:The influence of islet-activating protein (IAP), a Bordetella pertussis toxin, was studied on adenylate cyclase and GTPase activities in rat adipocyte membranes. Pretreatment of rats or intact rat adipocytes with IAP did not affect adenylate cyclase inhibition by the stable GTP analog, GTP gamma S, whereas inhibition ...

journal_title：FEBS letters

authors： Aktories K,Schultz G,Jakobs KH

更新日期：1983-05-30 00:00:00

abstract：:All eukaryotic cells enclose their genome within a dedicated, membrane-bound organelle termed the nucleus, which functions to partition gene transcription from sites of protein translation in the cytoplasm. Despite a great deal of research effort, basic questions about chromosome structure and gene expression mechanis...

journal_title：FEBS letters

authors： Trinkle-Mulcahy L,Lamond AI

更新日期：2008-06-18 00:00:00

abstract：:Lateral mobility of the vasopressin renal-type V2-receptor was investigated in LLC-PK1 porcine epithelial cells using the technique of fluorescence microphotolysis (photobleaching) and a rhodamine-labelled vasopressin analogue. At various times after ligand addition, cells were analyzed for both receptor lateral mobil...

journal_title：FEBS letters

authors： Jans DA,Peters R,Fahrenholz F

更新日期：1990-11-12 00:00:00

abstract：:Cytosolic NADP(+)-dependent malic enzyme (ME) from human tumor cells was characterized in detail and compared to ME from normal human tissues produced in recombinant E. coli. Kinetic properties, size as seen in SDS gels, and HPLC elution profiles of tryptic digests of human 'normal cell' ME and NADP(+)-ME from tumor c...

journal_title：FEBS letters

authors： Loeber G,Dworkin MB,Infante A,Ahorn H

更新日期：1994-05-16 00:00:00

abstract：:The NAD+-requiring enzymes of glycoside hydrolase family 4 (GHF4) contain a region with a conserved Gly-XXX-Gly-Ser (GXGS) motif near their N-termini that is reminiscent of the fingerprint region of the Rossmann fold, a conserved structural motif of classical nicotinamide nucleotide-binding proteins. The function of t...

journal_title：FEBS letters

authors： Raasch C,Armbrecht M,Streit W,Höcker B,Sträter N,Liebl W

更新日期：2002-04-24 00:00:00

abstract：:The physical properties of amino acids were investigated in order to evaluate their possible relationship to the assignment of codons for amino acids in the genetic code. A comparison of the interconversion probability between amino acids and the distances between the amino acids for individual physical properties rev...

journal_title：FEBS letters

authors： Sitaramam V

更新日期：1989-04-10 00:00:00

abstract：:Immunoblotting analysis was used to detect the microtubule-associated proteins present at different stages of rat brain development. Polyclonal antibodies were raised against the two main adult brain microtubule-associated proteins: MAP-2 (300 kDa) and TAU (60-70 kDa). Whatever the stage of development, anti-MAP-2 ser...

journal_title：FEBS letters

authors： Couchie D,Nunez J

更新日期：1985-09-02 00:00:00

abstract：:Sodium azide inhibited multi-site (steady-state) ATPase activity of E. coli F1 more than 90%, but did not affect uni-site (single-site) ATPase activity. Thus azide inhibited multi-site ATPase activity by lowering catalytic cooperativity. Consistent with this observation, azide changed the ligand-induced fluorescence r...

journal_title：FEBS letters

authors： Noumi T,Maeda M,Futai M

更新日期：1987-03-23 00:00:00

abstract：:Nitrogenase is a two-component metalloenzyme that catalyzes a MgATP hydrolysis driven reduction of substrates. Aluminum fluoride plus MgADP inhibits nitrogenase by stabilizing an intermediate of the on-enzyme MgATP hydrolysis reaction. We report here the redox properties and electron paramagnetic resonance (EPR) signa...

journal_title：FEBS letters

authors： Spee JH,Arendsen AF,Wassink H,Marritt SJ,Hagen WR,Haaker H

更新日期：1998-07-31 00:00:00

abstract：:Isolated mitotic chromosomes are able to form complexes with phosphatidylcholine liposomes in the presence and absence of Ca2+ ions, in the latter case in the presence of polyamines. Interactions with chromosomes stimulates liposome fusion. The fusion is promoted by condensed and EDTA-decondensed chromosomes. ...

journal_title：FEBS letters

authors： Balakireva LA,Belyaev ND,Budker VG,Kiseleva EV

更新日期：1991-06-24 00:00:00

abstract：:The in vivo toxicity of vanadium(V) has been found to correlate with the depletion of cellular glutathione and related non-protein thiols. With a view to understanding the mechanism for this observation, we have investigated the oxidation of glutathione, cysteine N-acetylcysteine and penicillamine by vanadium(V), usin...

journal_title：FEBS letters

authors： Shi XL,Sun XY,Dalal NS

更新日期：1990-10-01 00:00:00

abstract：:A mutant of Escherichia coli has been described that produces an altered form of penicillin-binding protein 5 which still binds penicillin but is unable to catalyse the release of the bound penicilloyl moiety. We show that the mutation is caused by a single nucleotide transition that results in a change from glycine a...

journal_title：FEBS letters

authors： Broome-Smith J,Spratt BG

更新日期：1984-01-09 00:00:00

abstract：:The colony-forming ability of catalase-deficient Escherichia coli mutant genetically modified to produce eicosapentaenoic acid (EPA) showed less decrease than in a control strain producing no EPA, when treated with 0.3mM hydrogen peroxide (H(2)O(2)) under non-growth conditions. H(2)O(2)-induced protein carbonylation w...

journal_title：FEBS letters

authors： Nishida T,Orikasa Y,Watanabe K,Okuyama H

更新日期：2006-12-11 00:00:00

abstract：:Mutations of the cysteines (positions 668 and 714) were generated in the IF2 C domain of Bacillus stearothermophilus translation initiation factor IF2. The corresponding proteins were characterized functionally and structurally. Most (yet not all) amino acid replacements at both positions resulted in severe reduction ...

journal_title：FEBS letters

authors： Misselwitz R,Welfle K,Krafft C,Welfle H,Brandi L,Caserta E,Gualerzi CO

更新日期：1999-10-15 00:00:00

abstract：:Ca(2+) channels that underlie mitochondrial Ca(2+) transport first reported decades ago have now just recently been precisely characterized electrophysiologically. Numerous data indicate that mitochondrial Ca(2+) uptake via these channels regulates multiple intracellular processes by shaping cytosolic and mitochondria...

journal_title：FEBS letters

authors： Ryu SY,Beutner G,Dirksen RT,Kinnally KW,Sheu SS

更新日期：2010-05-17 00:00:00

abstract：:The early response to bacterial inoculation has been investigated and two Arabidopsis genes, ap3.3a and ap4.3a have been characterized. The AP3.3A protein showed high identity to centrin, a ubiquitous cytoskeletal protein first identified in unicellular green alga. Amino-acid sequence analyses of the AP4.3A protein in...

journal_title：FEBS letters

authors： Cordeiro MC,Piqueras R,de Oliveira DE,Castresana C

更新日期：1998-09-04 00:00:00

abstract：:The scorpion toxin peptide BeKm-1 was synthesised by fluorenylmethoxycarbonyl solid phase chemistry and folded by air oxidation. The peptide's effects on heterologous human ether-a-go-go-related gene potassium current (I(HERG)) in HEK293 cells were assessed using 'whole-cell' patch clamp. Blockade of I(HERG) by BeKm-1...

journal_title：FEBS letters

authors： Milnes JT,Dempsey CE,Ridley JM,Crociani O,Arcangeli A,Hancox JC,Witchel HJ

更新日期：2003-07-17 00:00:00

abstract：:There is a paucity of information on the unique components that pathogens use to form respiratory chains. It is not known why mycobacteria encode multiple succinate dehydrogenases (SDHs) to perform menaquinone-linked succinate oxidation, a thermodynamically unfavorable reaction (ΔG° = +21 kJ·mol-1 ). In other bacteria...

journal_title：FEBS letters

authors： Hards K,Rodriguez SM,Cairns C,Cook GM

更新日期：2019-03-01 00:00:00