Cloning and expression of the epsilon 4 subunit of the NMDA receptor channel.

Abstract:

:The primary structure of a novel subunit of the mouse NMDA (N-methyl-D-aspartate) receptor channel, designated epsilon 4, has been revealed by cloning and sequencing the cDNA. The epsilon 4 subunit shares high amino acid sequence identity with the epsilon 1, epsilon 2 and epsilon 3 subunits of the mouse NMDA receptor channel, thus constituting the epsilon subfamily of the glutamate receptor channel. Expression from cloned cDNAs of the epsilon 4 subunit together with the zeta 1 subunit in Xenopus oocytes yields functional NMDA receptor channels. The epsilon 4/zeta 1 heteromeric channel exhibits high apparent affinities for agonists and low sensitivities to competitive antagonists. The epsilon 4 subunit is thus distinct in functional properties from the epsilon 1, epsilon 2 and epsilon 3 subunits, and contributes further diversity of the NMDA receptor channel.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Ikeda K,Nagasawa M,Mori H,Araki K,Sakimura K,Watanabe M,Inoue Y,Mishina M

doi

10.1016/0014-5793(92)81178-o

keywords:

subject

Has Abstract

pub_date

1992-11-16 00:00:00

pages

34-8

issue

1

eissn

0014-5793

issn

1873-3468

pii

0014-5793(92)81178-O

journal_volume

313

pub_type

杂志文章
  • The high molecular weight isoforms of basic fibroblast growth factor (FGF-2): an insight into an intracrine mechanism.

    abstract::Basic fibroblast growth factor (FGF-2) is an important modulator of cell growth and differentiation under both physiological and pathological conditions. Until recently, most investigations into the FGF-2 signalling pathway were concerned with its interaction with specific membrane receptors. Nevertheless, while a 18 ...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/s0014-5793(00)01189-3

    authors: Delrieu I

    更新日期:2000-02-18 00:00:00

  • Dominant-negative effect of mutant valosin-containing protein in aggresome formation.

    abstract::Lewy bodies (LBs) are the pathologic hallmark of Parkinson's disease. Recent studies revealed that LBs exhibit several morphologic and molecular similarities to aggresomes. Aggresomes are perinuclear aggregates representing intracellular deposits of misfolded proteins. Recently, valosin-containing protein (VCP) was on...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2005.12.044

    authors: Kitami MI,Kitami T,Nagahama M,Tagaya M,Hori S,Kakizuka A,Mizuno Y,Hattori N

    更新日期:2006-01-23 00:00:00

  • Apamin-sensitive potassium channels mediate agonist-induced oscillations of membrane potential in pituitary gonadotrophs.

    abstract::In cultured rat pituitary gonadotrophs, gonadotropin-releasing hormone (GnRH) induces rapid hyperpolarization of the cell membrane and causes cessation of the spontaneous electrical activity present in non-stimulated cells. This initial response to GnRH is followed by slow oscillations of membrane potential (Vm) which...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(92)80201-q

    authors: Kukuljan M,Stojilković SS,Rojas E,Catt KJ

    更新日期:1992-04-13 00:00:00

  • Epithelial CaSR deficiency alters intestinal integrity and promotes proinflammatory immune responses.

    abstract::The intestinal epithelium is equipped with sensing receptor mechanisms that interact with luminal microorganisms and nutrients to regulate barrier function and gut immune responses, thereby maintaining intestinal homeostasis. Herein, we clarify the role of the extracellular calcium-sensing receptor (CaSR) using intest...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2014.05.007

    authors: Cheng SX,Lightfoot YL,Yang T,Zadeh M,Tang L,Sahay B,Wang GP,Owen JL,Mohamadzadeh M

    更新日期:2014-11-17 00:00:00

  • Protein arginine methyl transferases-3 and -5 increase cell surface expression of cardiac sodium channel.

    abstract::The α-subunit of the cardiac voltage-gated sodium channel (NaV1.5) plays a central role in cardiomyocyte excitability. We have recently reported that NaV1.5 is post-translationally modified by arginine methylation. Here, we aimed to identify the enzymes that methylate NaV1.5, and to describe the role of arginine methy...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2013.07.043

    authors: Beltran-Alvarez P,Espejo A,Schmauder R,Beltran C,Mrowka R,Linke T,Batlle M,Pérez-Villa F,Pérez GJ,Scornik FS,Benndorf K,Pagans S,Zimmer T,Brugada R

    更新日期:2013-10-01 00:00:00

  • HY5 regulates anthocyanin biosynthesis by inducing the transcriptional activation of the MYB75/PAP1 transcription factor in Arabidopsis.

    abstract::Several positive transcription factors regulate Arabidopsis anthocyanin biosynthesis. HY5, a component of light-signaling pathways, and PAP1, an R2R3-MYB transcription factor, share common regulatory targets on anthocyanin biosynthesis genes. The epistatic interactions between the two transcription factors are current...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2013.03.037

    authors: Shin DH,Choi M,Kim K,Bang G,Cho M,Choi SB,Choi G,Park YI

    更新日期:2013-05-21 00:00:00

  • Beneficial modulation of the gut microbiota.

    abstract::The human gut microbiota comprises approximately 100 trillion microbial cells and has a significant effect on many aspects of human physiology including metabolism, nutrient absorption and immune function. Disruption of this population has been implicated in many conditions and diseases, including examples such as obe...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/j.febslet.2014.03.035

    authors: Walsh CJ,Guinane CM,O'Toole PW,Cotter PD

    更新日期:2014-11-17 00:00:00

  • Mutations in the amino-terminal domain of the human poly(ADP-ribose) polymerase that affect its catalytic activity but not its DNA binding capacity.

    abstract::Poly-ADP ribosylation of nuclear proteins is activated when poly(ADP-ribose) polymerase (PARP), a nuclear zinc-finger enzyme, binds to single-strand DNA breaks. To understand how the signal emerging from its DNA-binding domain (DBD) bound to such breaks is transduced to its catalytic domain, the structure-function rel...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(96)01347-6

    authors: Trucco C,Flatter E,Fribourg S,de Murcia G,Ménissier-de Murcia J

    更新日期:1996-12-16 00:00:00

  • The effect of local anaesthetics on the ryanodine receptor/Ca2+ release channel of brain microsomal membranes.

    abstract::The effects of various local anaesthetics (LAs) on ryanodine binding of the sheep brain ryanodine receptor were tested. Tetracaine and dibucaine inhibit the binding with half-maximal inhibition (CI50) of 0.12 mM and 0.7 mM, respectively. Lidocaine and its analog QX-314, on the other hand, stimulate the binding up to 3...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)80969-2

    authors: Martin C,Ashley R,Shoshan-Barmatz V

    更新日期:1993-08-09 00:00:00

  • Activation of muscarinic K+ current by beta-adrenergic receptors in cultured atrial myocytes transfected with beta1 subunit of heterotrimeric G proteins.

    abstract::Muscarinic K+ channels (IK(ACh)) in native atrial myocytes are activated by betagamma subunits of pertussis toxin (Ptx)-sensitive heterotrimeric G proteins coupled to different receptors. betagamma subunits of Ptx-insensitive Gs, coupled to beta-adrenergic receptors, do not activate native IK(ACh). In atrial myocytes ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(98)01350-7

    authors: Bender K,Wellner-Kienitz MC,Meyer T,Pott L

    更新日期:1998-11-13 00:00:00

  • Cytoplasmic pH, a key determinant of growth factor-induced DNA synthesis in quiescent fibroblasts.

    abstract::In response to growth factors, quiescent fibroblast mutants lacking Na+/H+ exchange activity fail to elevate their cytoplasmic pH (pHi) and to reinitiate DNA synthesis at neutral and acidic pHo. A pHi threshold of approximately 7.2 exists, below which growth factors cannot set in motion the Go to S phase transition. R...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(85)80439-7

    authors: Pouysségur J,Franchi A,L'Allemain G,Paris S

    更新日期:1985-10-07 00:00:00

  • Translational approaches to restoring mitochondrial function in Parkinson's disease.

    abstract::There is strong evidence of a key role for mitochondrial dysfunction in both sporadic and all forms of familial Parkinson's disease (PD). However, none of the clinical trials carried out with putative mitochondrial rescue agents have been successful. Firm establishment of a wet biomarker or a reliable readout from ima...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1002/1873-3468.12920

    authors: Mortiboys H,Macdonald R,Payne T,Sassani M,Jenkins T,Bandmann O

    更新日期:2018-03-01 00:00:00

  • Band-3 protein-mediated anion conductance of the red cell membrane. Slippage vs ionic diffusion.

    abstract::The band 3 protein-mediated, valinomycin-induced KCl efflux continues to increase with increasing [KCl] when the Cl-/Cl- equilibrium exchange becomes saturated. This suggests the existence of a band 3-mediated component of Cl- flux that contributes to the electrical conductance without being related to slippage; i.e.,...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(83)80272-5

    authors: Kaplan JH,Pring M,Passow H

    更新日期:1983-05-30 00:00:00

  • Putrescine may be a natural stimulator of glucose-6-phosphate dehydrogenase.

    abstract::The possible relationship between pyridoxal phosphate-dependent ornithine decarboxylase (ODC) activity and glucose-6-phosphate dehydrogenase (G6PD) activity has been studied in the osteoblasts of the growth-plate of metatarsals of rats fed a pyridoxine-deficient diet, which caused depressed G6PD levels. The G6PD activ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(86)80579-8

    authors: Dodds RA,Dunham J,Bitensky L,Chayen J

    更新日期:1986-05-26 00:00:00

  • The rat beta 1-subunit of the GABAA receptor forms a picrotoxin-sensitive anion channel open in the absence of GABA.

    abstract::The structural basis of GABA-gated chloride channels in mammalian brain is presently explored by the functional expression of cDNAs coding for the alpha, beta or gamma-subunits of the receptor and their isoforms. In this context, we expressed the cloned cDNA coding for the rat beta 1-subunit of the GABAA receptor in t...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(89)81576-5

    authors: Sigel E,Baur R,Malherbe P,Möhler H

    更新日期:1989-11-06 00:00:00

  • The Drosophila cation channel trpl expressed in insect Sf9 cells is stimulated by agonists of G-protein-coupled receptors.

    abstract::Structures and regulations of vertebrate channels responsible for sustained calcium elevations after hormone stimulation are largely unknown. Therefore, the Drosophila photoreceptor channels, trp and trpl, which are assumed to be involved in calcium influx, serve as model system, trpl expressed in Sf9 cells showed spo...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(94)01455-a

    authors: Harteneck C,Obukhov AG,Zobel A,Kalkbrenner F,Schultz G

    更新日期:1995-01-30 00:00:00

  • CLC transport proteins in plants.

    abstract::Nitrate compartmentalization in intracellular organelles has been long recognized as critical for plant physiology but the molecular identity of the proteins involved remained unclear for a long time. In Arabidopsis thaliana, AtClC-a has been recently shown to be a NO(3)(-)/H(+) antiporter critical for nitrate transpo...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/j.febslet.2009.12.042

    authors: Zifarelli G,Pusch M

    更新日期:2010-05-17 00:00:00

  • IL-1 beta, a strong mediator for glucose uptake by rheumatoid and non-rheumatoid cultured human synoviocytes.

    abstract::Higher basal 2-deoxy-D-glucose uptake in rheumatoid synovial cells than in non-rheumatoid synovial cells, was found to be associated with an increased interleukin-1 beta (IL-1 beta) secretion (respectively 850 +/- 238 vs. 8.3 +/- 2.4 pg/24 h/10(5) cells, mean +/- S.E.M.). When exogenous human recombinant IL-1 beta was...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(92)80481-u

    authors: Hernvann A,Aussel C,Cynober L,Moatti N,Ekindjian OG

    更新日期:1992-05-25 00:00:00

  • Asymmetries in kinesin-2 and cytoplasmic dynein contributions to melanosome transport.

    abstract::The mechanisms involved in bidirectional transport along microtubules remain largely unknown. We explored the collective action of kinesin-2 and dynein motors during transport of melanosomes in Xenopus laevis melanophores. These motors are attached to organelles through accessory proteins establishing a complex molecu...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2015.07.038

    authors: De Rossi MC,De Rossi ME,Sued M,Rodríguez D,Bruno L,Levi V

    更新日期:2015-09-14 00:00:00

  • A human synaptic vesicle monoamine transporter cDNA predicts posttranslational modifications, reveals chromosome 10 gene localization and identifies TaqI RFLPs.

    abstract::A human vesicular monoamine transporter cDNA has been identified by screening a human brainstem library using sequences from the rat brain synaptic vesicle monoamine transporter (SVMT) [(1992) Cell 70, 539-551; (1992) Proc. Natl. Acad. Sci. USA 89, 10993-10997]. The hSVMT shares 92% amino acid identity with the rat se...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)80539-7

    authors: Surratt CK,Persico AM,Yang XD,Edgar SR,Bird GS,Hawkins AL,Griffin CA,Li X,Jabs EW,Uhl GR

    更新日期:1993-03-08 00:00:00

  • The antioxidative function of eicosapentaenoic acid in a marine bacterium, Shewanella marinintestina IK-1.

    abstract::When the eicosapentaenoic acid (EPA)-deficient mutant strain IK-1Delta8 of the marine EPA-producing Shewanella marinintestina IK-1 was treated with various concentrations of hydrogen peroxide (H(2)O(2)), its colony-forming ability decreased more than that of the wild type. Protein carbonylation, induced by treating ce...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2007.07.065

    authors: Nishida T,Morita N,Yano Y,Orikasa Y,Okuyama H

    更新日期:2007-09-04 00:00:00

  • The inhibition of restriction endonucleases due to Z-DNA in negatively supercoiled plasmid.

    abstract::Plasmid pGC20 containing the (dGC)9 insert in SmaI recognition site has been used to study the inhibition of cleavage by different restriction endonuclease due to Z-DNA formation in (dCG)10 sequence of the negatively supercoiled plasmid. Data obtained indicate the different sensitivity of restriction endonucleases to ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)80211-k

    authors: Lesnik EA,Bes'chetnikova ZhA,Maslova RN,Varshavsky JaM

    更新日期:1991-03-11 00:00:00

  • Isoform-specific interaction of pyruvate kinase with hepatitis C virus NS5B.

    abstract::Hepatitis C virus (HCV) replication and pathogenesis involve both virus-encoded proteins and cellular factors. In our study, we showed that NS5B, the HCV RNA-dependent RNA polymerase, interacted with M2 type pyruvate kinase (M2PK) but not L type pyruvate kinase. We confirmed the interaction by GST pull down, coimmunop...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2008.05.033

    authors: Wu X,Zhou Y,Zhang K,Liu Q,Guo D

    更新日期:2008-06-25 00:00:00

  • Insertion of MOM22 into the mitochondrial outer membrane strictly depends on surface receptors.

    abstract::Targeting of preproteins to mitochondria and their translocation across the outer membrane are mediated by the mitochondrial receptor complex. This protein complex contains the import receptors MOM19 and MOM72 and the general insertion pore GIP. All seven components of the receptor complex are synthesized in the cytos...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)80107-6

    authors: Keil P,Pfanner N

    更新日期:1993-04-26 00:00:00

  • Conformational responses of an arachidonate- and U46619-binding haemoprotein in relation to platelet activation.

    abstract::Antibodies were raised, in rabbits, against an arachidonate- and U46619-binding protein purified from calf platelets. Spectral measurements and immunodiffusion experiments were employed to follow conformational responses of the protein in relation to platelet activation. Upon treatment with the platelet agonists, arac...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(87)81347-9

    authors: Krishnan LK,Jamaluddin MP

    更新日期:1987-02-23 00:00:00

  • Thermostable chimeric PQQ glucose dehydrogenase.

    abstract::The thermal stability of PQQ glucose dehydrogenases (PQQGDHs) which were chimeras with more than 95% made up of the N-terminal region of Escherichia coli PQQGDH and the rest made up of the C-terminal region of Acinetobacter calcoaceticus PQQGDH was investigated. Among the chimeric PQQGDHs, E97A3 (E. coli 97% and A. ca...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(95)00418-9

    authors: Sode K,Watanabe K,Ito S,Matsumura K,Kikuchi T

    更新日期:1995-05-15 00:00:00

  • Reaction of nitric oxide with hydrogen peroxide to produce potentially cytotoxic singlet oxygen as a model for nitric oxide-mediated killing.

    abstract::Nitric oxide, as well as being a major regulator of vascular reactivity, has been shown to be one of the mediators of cytotoxicity in macrophages. This cytotoxic effect seems to be due to the interaction between nitric oxide and oxygen-related free radicals. This study shows that, in vitro, nitric oxide reacts with hy...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)80621-z

    authors: Noronha-Dutra AA,Epperlein MM,Woolf N

    更新日期:1993-04-19 00:00:00

  • Werner syndrome protein associates with gamma H2AX in a manner that depends upon Nbs1.

    abstract::The WRN protein is mutated in the chromosomally unstable Werner syndrome (WS) and the Nbs1 protein is mutated in Nijmegen breakage syndrome (NBS). The Nbs1 protein is an integral component of the M/R/N complex. Although WRN is known to interact with this complex in response to gamma-irradiation, the mechanism of actio...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2005.01.028

    authors: Cheng WH,Sakamoto S,Fox JT,Komatsu K,Carney J,Bohr VA

    更新日期:2005-02-28 00:00:00

  • Flash-induced voltage changes in halorhodopsin from Natronobacterium pharaonis.

    abstract::The flash-induced voltage response of halorhodopsin at high NaCl concentration comprises two main kinetic components. The first component with tau approximately 1 micros does not exceed 4% of the overall response amplitude and is probably associated with the formation of the L (hR520) intermediate. The second main com...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(98)00394-9

    authors: Kalaidzidis IV,Kalaidzidis YL,Kaulen AD

    更新日期:1998-05-01 00:00:00

  • Inhibition of electron transport of rat liver mitochondria by unnatural (-)-antimycin A3.

    abstract::The inhibition of electron transport by unnatural (-)-antimycin A3 was examined with rat liver mitochondria and compared with that of natural (+)-antimycin A3. (-)-Antimycin A3 inhibited respiration about 1/100th as strongly as natural (+)-antimycin A3. (-)-Antimycin A3 binding to the cytochrome bc1 complex did not se...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)80834-p

    authors: Miyoshi H,Kondo H,Oritani T,Saitoh I,Iwamura H

    更新日期:1991-11-04 00:00:00