Abstract:
:Immunoblotting analysis was used to detect the microtubule-associated proteins present at different stages of rat brain development. Polyclonal antibodies were raised against the two main adult brain microtubule-associated proteins: MAP-2 (300 kDa) and TAU (60-70 kDa). Whatever the stage of development, anti-MAP-2 serum detected high molecular mass proteins and at immature stages a protein of 62 kDa. This protein which has previously been referred to as 'young TAU slow' is, therefore, immunologically related to MAP-2. The anti-TAU serum (but not the anti-MAP-2 serum) detected at immature stages of development a 48 kDa protein which also disappears at adulthood. This 48 kDa entity which has been referred to as 'young TAU fast' is progressively replaced by the closely spaced bands (60-70 kDa) of adult TAU proteins. The 62 and 48 kDa proteins appear therefore to be immunologically distinct and represent two microtubule-associated proteins specific to the immature brain.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Couchie D,Nunez Jdoi
10.1016/0014-5793(85)80397-5subject
Has Abstractpub_date
1985-09-02 00:00:00pages
331-5issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(85)80397-5journal_volume
188pub_type
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