Abstract:
:Recent three-dimensional structures of helical membrane proteins present new challenges for the prediction of structure from amino acid sequence. Membrane proteins reside stably in a thermodynamic free energy minimum after release into the membrane's bilayer fabric from the translocon complex. This means that structure prediction is primarily a problem of physical chemistry. But the folding processes within the translocon must also be considered. A distilled overview of the physical principles of membrane protein stability is presented, and extended to encompass translocon-assisted folding.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
White SHdoi
10.1016/s0014-5793(03)01153-0keywords:
subject
Has Abstractpub_date
2003-11-27 00:00:00pages
116-21issue
1eissn
0014-5793issn
1873-3468pii
S0014579303011530journal_volume
555pub_type
杂志文章,评审相关文献
FEBS LETTERS文献大全abstract::Recent studies show LDL receptor-related protein 1B, LRP1B as a transducer of extracellular signals. Here, we identify six interacting partners of the LRP1B cytoplasmic region by yeast two-hybrid screen and confirmed their in vivo binding by immunoprecipitation. One of the partners, PICK1 recognizes the C-terminus of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.11.045
更新日期:2009-01-05 00:00:00
abstract::Incubation of rhodanese with hche aperonins GroEL and GroES (1:2 GroEL14:GroES7 molar ratio) under functional and steady state conditions for ATP leads to the formation of a high proportion of rhodanese-bound symmetric complexes (GroEL14(GroES7)2), as revealed by native electrophoresis. Aliquots of such samples were o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00186-5
更新日期:1997-03-24 00:00:00
abstract::We have studied whether the cooperative insecticidal effect of certain scorpion toxin pairs, namely either a combination of excitatory and depressant, or alpha and depressant scorpion toxins, would improve the efficacy of Autographa californica nucleopolyhedrovirus (AcMNPV) over a virus expressing only a single toxin,...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00104-2
更新日期:2003-02-27 00:00:00
abstract::An antibody recognising acetylated core histones was used to immunoprecipitate chromatin fragments from proliferating human K562 cells and from cells induced to differentiate with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). The DNA of the acetylated chromatin was probed with sequences of platelet der...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81601-u
更新日期:1993-12-20 00:00:00
abstract::Hemin stimulates cAMP production in human peripheral blood mononuclear cells (PBMC). The kinetics are similar to that of hormone-induced cAMP generation, namely a rapid effect followed by a desensitization phase. Several experimental findings suggest that prostaglandins do not mediate this effect. First, macrophage de...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80529-p
更新日期:1992-06-01 00:00:00
abstract::Mitochondrial uncoupling protein 2 (UCP2) is abundant in developing monocyte/macrophage cells and may affect hematopoiesis by reducing formation of reactive oxygen species. The aims of this study were to further characterize the involvement of UCP2 in hematopoiesis. In situ hybridization in mouse embryos identified UC...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.02.012
更新日期:2007-03-20 00:00:00
abstract::Cytochrome oxidase exhibits phosphorescence from tryptophan in aqueous solution in the absence of oxygen. The lifetime for the resting reduced enzyme suspended in Tween-20 is around 30 ms at pH 8. The lifetime is longest between pH 7 and 8 and decreases with lowering of pH. Oxygen quenches the phosphorescence with a S...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80566-l
更新日期:1991-05-20 00:00:00
abstract::The diffusion of fluorescein isothiocyanate-labelled dextran molecules in suspensions of centrifugally, tightly packed, erythrocyte ghosts was measured by fluorescence recovery after photobleaching. In comparison with diffusion in aqueous solution, the diffusion coefficients for probe molecules of varying size were ab...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81000-7
更新日期:1988-12-19 00:00:00
abstract::The delta psi of R. sphaeroides, grown under high light to reduce the levels of light-harvesting bacteriochlorophyll, was naturally manipulated using light intensity. The relationship between delta psi and the swimming speed of free swimming populations of cells was investigated. After de-energisation by incubation in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00572-9
更新日期:1994-07-04 00:00:00
abstract::Rat brain microtubules were prepared at the adult stage and from immature (i.e., 4-day-old) animals. At an early stage of development, the composition of microtubule-associated proteins is qualitatively different from that found at the adult stage [(1982) Eur. J. Biochem. 129, 465-471]. The influence of calmodulin on ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81148-5
更新日期:1984-07-09 00:00:00
abstract::A human insulinoma cDNA (PC2) that encodes a protein homologous to the Kex2/subtilisin-like proteinases has recently been described [1990, J. Biol. Chem. 265, 2997-3000]. In order to characterise the associated proteinase activity, mRNA encoding PC2 was synthesised in vitro and microinjected into Xenopus oocytes. The ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80703-6
更新日期:1991-06-24 00:00:00
abstract::A rapid and efficient method for the purification of malate synthase, an enzyme uniquely confined to glyoxysomes, from cotyledons of Brassica napus L. has been developed. The two step purification procedure is based on the consequent utilization of the tendency of malate synthase to form high molecular weight aggregat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01114-t
更新日期:1995-10-30 00:00:00
abstract::The Surfeit locus is an unusual cluster of at least 6 housekeeping genes whose organisation is conserved between birds and mammals. We have previously shown that the divergently transcribed Surf-1 and Surf-2 genes are separated by a bi-directional, TATA-less promoter. In mouse, the Surf-1/Surf-2 promoter contains thre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00567-2
更新日期:1994-06-27 00:00:00
abstract::Phosphorylations catalyzed by cAMP-dependent protein kinase are essential for sperm motility, and type II cAMP-dependent protein kinase in mature sperm has been shown to be firmly bound to the flagellum via the regulatory subunit, RII. The present study documents high-levelled expression of a human, testis-specific RI...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80253-4
更新日期:1989-03-27 00:00:00
abstract::Mutations in the nuclear matrix protein Matrin 3 (MATR3) have been identified in amyotrophic lateral sclerosis and myopathy. To investigate the mechanisms underlying MATR3 mutations in neuromuscular diseases and efficiently screen for modifiers of MATR3 toxicity, we generated transgenic MATR3 flies. Our findings indic...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13858
更新日期:2020-09-01 00:00:00
abstract::It has long been known from fluorescence recovery after photobleaching experiments that the mobility of most cell surface receptors is much smaller than expected for free diffusion of proteins in a fluid lipid bilayer. Single-particle tracking experiments are currently revealing the complexity of the constraints to fr...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(98)00595-x
更新日期:1998-06-23 00:00:00
abstract::The GTPase RhoA is essential for the development of pre-T cells in the thymus. To investigate the mechanisms used by RhoA to control thymocyte development we have used Affymetrix gene profiling to identify RhoA regulated genes in T cell progenitors. The data show that RhoA plays a specific and essential role in pre-T ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.07.077
更新日期:2007-09-04 00:00:00
abstract::Trypsin treatment of HeLa cells results in a limited proteolysis of the coxsackievirus and adenovirus receptor (CAR) after which the cleaved CAR remains cell-associated and tryptic peptides remain associated through disulfide bonds. Trypsin-treated HeLa cells remain susceptible to infection with coxsackievirus B and p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02144-x
更新日期:2000-11-03 00:00:00
abstract::We previously reported that daily intraperitoneal injections of oleamide weakly inhibits the spontaneous metastasis of BL6 cells by blocking the gap junction-mediated intercellular communications (GJIC) of connexin 26 (Cx26). In the present study, we tested camellia oil, olive oil and cottonseed oil which are rich in ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.04.080
更新日期:2007-05-29 00:00:00
abstract::A-to-I RNA editing can lead to recoding of pre-mRNAs with profound functional consequences for the ensuing proteins. Here we show that complement component 1, q subcomponent-like 1 (C1QL1) undergoes RNA editing in vivo causing non-synonymous amino acid substitutions in human, mouse as well as zebrafish. The major edit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.02.044
更新日期:2009-04-02 00:00:00
abstract::Adrenomedullin, a potently hypotensive peptide isolated from human pheochromocytoma, is known to elicit a rise in cAMP levels within mammalian endothelial and smooth muscle cells. Until now, however, little has been known about the adrenomedullin receptor. Recently, a group called receptor activity-modifying proteins ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00358-0
更新日期:1999-04-01 00:00:00
abstract::A number of human diseases can be linked to aberrations in protein folding which cause an imbalance in protein homeostasis. Molecular chaperones, including heat shock proteins, act to assist protein folding, stability and activity in the cell. Attention has begun to focus on modulating the expression and/or activity o...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2007.05.040
更新日期:2007-07-31 00:00:00
abstract::Stimulated production of reactive oxygen species (ROS) by plasma membrane-associated nicotinamide adenine dinucleotide phosphate oxidases (Nox) in non-phagocytic cells regulates a number of biological processes including growth, vessel tone, and oxygen sensing. The purpose of this study was to investigate H(2)O(2)-sti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.03.057
更新日期:2005-04-25 00:00:00
abstract::Fibroblast growth factors (FGFs) and their receptors are expressed in a variety of mammalian tissues, playing a role in development and cell proliferation. While analyzing human sperm motility, we found that sperm treated with endo-β-galactosidase (EBG), which specifically hydrolyzes poly-N-acetyllactosamine type glyc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.07.056
更新日期:2013-10-01 00:00:00
abstract::We have used a panel of monoclonal antibodies in a study of the expression of multiple tubulins in Physarum polycephalum. Three anti-beta-tubulin monoclonal antibodies, DM1B, DM3B3 and KMX-1 all reacted with the beta 1-tubulin isotypes expressed in both myxamoebae and plasmodia. However, these antibodies showed a spec...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81244-8
更新日期:1985-08-05 00:00:00
abstract::On incubation of 7-day-old wheat seedlings in the presence of [3H]thymidine, the radioactivity incorporated into coleoptile DNA is found to be localized mainly (greater than 95%) in the fraction of heavy mitochondrial DNA (H-mt DNA; rho = 1.716 gm/cm3). Upon long (48-72 h) incubation of cut-off seedlings in water, the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80033-d
更新日期:1992-02-24 00:00:00
abstract::Epigallocatechin-3-gallate (EGCG) is a major component of green tea polyphenols which displays potential properties of anticancer and neuroprotection. Here we show that protection of motor neuron by EGCG is associated with regulating glutamate level in organotypic culture of rat spinal cord. In this model, EGCG blocke...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.05.011
更新日期:2010-07-02 00:00:00
abstract::Syndecans are transmembrane heparan sulfate proteoglycans, with roles in development, tumorigenesis and inflammation, and growing evidence for involvement in tissue regeneration. This is a fast developing field with the prospect of utilizing tissue engineering and biomaterials in novel therapies. Syndecan receptors ar...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12280
更新日期:2016-08-01 00:00:00
abstract::We examined the effects of histidine residues that were artificially introduced into complementarity-determining regions of antibodies on antigen-antibody interactions at different pH values. Using a monoclonal antibody specific for hen egg-white lysozyme and three mutant antibodies that contained a histidine residue,...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80745-3
更新日期:1992-08-31 00:00:00
abstract::In our study, transcriptome microarrays are used to identify differentially expressed miRNAs and mRNAs in cervical cancer specimens. We find that microRNA-145 (miR-145) expression is significantly decreased in cervical cancer tissues and cell lines, and is associated with advanced cancer stages, large tumor size and m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.01.037
更新日期:2015-03-12 00:00:00