Interaction between calmodulin and microtubule-associated proteins prepared at different stages of brain development.

abstract：:Human nuclear cyclophilin 33 (hCyP33) was the first protein which was found to contain an RNA-binding motif and a PPIase domain. It was not known what cellular and physiological roles are played by the RNA-binding activity as well as the PPIase activity of hCyP33. In this paper, we investigated the binding specificity...

journal_title：FEBS letters

authors： Wang Y,Han R,Zhang W,Yuan Y,Zhang X,Long Y,Mi H

更新日期：2008-03-05 00:00:00

abstract：:Bovine heart cytochrome-c oxidase was reconstituted in liposomes and the kinetics of cytochrome c oxidation were measured by the polarographic and photometric method under uncoupled conditions in the presence of various polyvalent anions. In order to distinguish between specific and unspecific ionic effects of ATP, th...

journal_title：FEBS letters

authors： Hüther FJ,Kadenbach B

更新日期：1986-10-20 00:00:00

abstract：:The white-rot fungus Phanerochaete chrysosporium has two intracellular beta-glucosidases (BGL1A and BGL1B) belonging to glycoside hydrolase (GH) family 1. BGL1B effectively hydrolyzes cellobiose and cellobionolactone, but BGL1A does not. We have determined the crystal structure of BGL1A in substrate-free and gluconola...

journal_title：FEBS letters

authors： Nijikken Y,Tsukada T,Igarashi K,Samejima M,Wakagi T,Shoun H,Fushinobu S

更新日期：2007-04-03 00:00:00

abstract：:An efficient method is presented for the production of intact mammalian prion proteins and partial sequences thereof. As an illustration we describe the production of polypeptides comprising residues 23-231, 81-231, 90-231 and 121-231 of the human prion protein (hPrP). Polypeptides were expressed as histidine tail fus...

journal_title：FEBS letters

authors： Zahn R,von Schroetter C,Wüthrich K

更新日期：1997-11-17 00:00:00

abstract：:Side chain-hydroxylated derivatives of cholesterol (OH sterol) inhibiting lymphoblastic transformation bind with high affinity and specificity to a hydroxysterol binding protein (OHSBP) in the cytosol of human lymphocytes. These binding properties of OHSBP suggested some analogies with that of steroid hormone receptor...

journal_title：FEBS letters

authors： Defay R,Astruc M,Beseme F,Descomps B,Crastes de Paulet A

更新日期：1984-08-06 00:00:00

abstract：:14-3-3 proteins were the first signaling molecules to be identified as discrete phosphoserine/threonine binding modules. This family of proteins, which includes seven isotypes in human cells and up to 15 in plants, plays critical roles in cell signaling events that control progress through the cell cycle, transcriptio...

journal_title：FEBS letters

authors： Yaffe MB

更新日期：2002-02-20 00:00:00

abstract：:Combining mutation and chemical modification, we have introduced Asp, Gln, Cys, S-carboxymethylcysteine (Cax) and S-carbamoylmethylcysteine (Cam) into the positions of Glu190 and Glu201 of the beta subunit of F1-ATPase from the thermophilic Bacillus PS3. The steady-state ATPase activities of alpha 3 beta 3 gamma compl...

journal_title：FEBS letters

authors： Amano T,Tozawa K,Yoshida M,Murakami H

更新日期：1994-07-04 00:00:00

abstract：:To date the only tRNAs containing nucleosides modified with a selenium (5-carboxymethylaminomethyl-2-selenouridine and 5-methylaminomethyl-2-selenouridine) have been found in bacteria. By using tRNA anticodon-stem-loop fragments containing S2U, Se2U, or geS2U, we found that in vitro tRNA 2-selenouridine synthase (SelU...

journal_title：FEBS letters

authors： Sierant M,Leszczynska G,Sadowska K,Komar P,Radzikowska-Cieciura E,Sochacka E,Nawrot B

更新日期：2018-07-01 00:00:00

abstract：:Genetically obese (ob/ob) mice develop a marked insulin resistance in brown adipose tissue soon after weaning, and this is paralleled by a fall in the acute activation of the mitochondrial proton conductance pathway in the tissue on cold exposure. Treatment of ob/ob mice with ciglitazone, a new oral hypoglycaemic, led...

journal_title：FEBS letters

authors： Mercer SW,Trayhurn P

更新日期：1986-01-20 00:00:00

abstract：:The cDNA for the nearly full-length rabbit muscle glycogen phosphorylase mRNA has been isolated and sequenced. The cDNA is rich in G and C nucleotides. This feature is especially striking at the 3rd position of codons, where 86% of the 843 amino acid codons terminate with G or C. Methionine, presumably the initiation ...

journal_title：FEBS letters

authors： Nakano K,Hwang PK,Fletterick RJ

更新日期：1986-08-18 00:00:00

abstract：:Specific receptors for angiotensin II (AII) were expressed in albino Xenopus laevis oocytes co-injected with poly(A)+ mRNA isolated from rat adrenal cortex and the calcium-specific photoprotein, aequorin. In such oocytes, AII elicited rapid, dose-dependent rises in cytosolic free calcium with light emission responses ...

journal_title：FEBS letters

authors： Sandberg K,Markwick AJ,Trinh DP,Catt KJ

更新日期：1988-12-05 00:00:00

abstract：:Plasma membrane, sarco-endoplasmic reticulum and secretory pathway Ca2+-ATPases (designated PMCA, SERCA and SPCA) regulate intracellular Ca2+ in animal cells. The presence of PMCA, and the absence of SERCA, in sea urchin sperm is known. By using inhibitors of Ca2+-ATPases, we now show the presence of SPCA and Ca2+ sto...

journal_title：FEBS letters

authors： Gunaratne HJ,Vacquier VD

更新日期：2006-07-10 00:00:00

abstract：:To study the fluctuations of cGMP in living cells through changes of energy transfer of dissociable fluorescence labeled subunits, we constructed a cGMP-sensitive probe by combining the N-terminus of the type I regulatory subunit of cAMP-dependent protein kinase (PKA) with the cGMP binding sites of cGMP-dependent prot...

journal_title：FEBS letters

authors： Wild N,Herberg FW,Hofmann F,Dostmann WR

更新日期：1995-11-06 00:00:00

abstract：:Activation of p53 plays a central role in the cell's response to various stress signals. We investigated whether p53 is activated upon disruption of actin microfilaments, caused by cytochalasin D (CD). We show that treatment with CD leads to accumulation of p53 in the cells and activation of p53-dependent transcriptio...

journal_title：FEBS letters

authors： Rubtsova SN,Kondratov RV,Kopnin PB,Chumakov PM,Kopnin BP,Vasiliev JM

更新日期：1998-07-03 00:00:00

abstract：:Drug carriers such as liposomes are not readily transported across cellular barriers that constitute epithelia. However, certain epithelia (rabbit yolk sac endoderm and enterocytes of suckling rat gut proximal small intestine) are well known to transcytose maternal IgG by Fc receptor-mediated endocytic events. We have...

journal_title：FEBS letters

authors： Patel HM,Wild AE

更新日期：1988-07-18 00:00:00

abstract：:Two protein phosphatases of 103 and 29 kDa as determined by gel filtration, were purified from hen oviducts. The 103 -kDa phosphatase was purified 7300-fold to near homogeneity and dissociated into two polypeptides in the presence of SDS. Molecular masses of these polypeptides were estimated to be 60 and 38 kDa by SDS...

journal_title：FEBS letters

authors： Kanayama K,Wada K,Negami A,Yamamura H,Tanabe T

更新日期：1985-05-06 00:00:00

abstract：:Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system characterized by widespread inflammation, focal demyelination and a variable degree of axonal and neuronal loss. Ionic conductances regulate T cell activation as well as neuronal function and thus have been found to play a crucial ...

journal_title：FEBS letters

authors： Ehling P,Bittner S,Budde T,Wiendl H,Meuth SG

更新日期：2011-12-01 00:00:00

abstract：:In bovine adrenocortical cells, the fatty acyl chains of the phospholipids have been identified in the membranes of the different cell compartments: plasma membranes, Golgi complex and coated vesicle membranes. An increase in the total number of unsaturation in the fatty acid is demonstrated in the coated vesicle memb...

journal_title：FEBS letters

authors： de Paillerets C,Bomsel M,Weintraub H,Pépin D,Alfsen A

更新日期：1987-07-13 00:00:00

abstract：:It had been shown previously by heterologous expression in Schizosaccharomyces pombe, that the two monosaccharide/H+ symporters HUP1 and HUP2 of Chlorella kessleri differ significantly concerning their substrate specificity: HUP1 transports predominantly D-glucose while HUP2 prefers D-galactose. Several chimeric trans...

journal_title：FEBS letters

authors： Will A,Tanner W

更新日期：1996-02-26 00:00:00

abstract：:Isoenzymes of creatine kinase (CK, EC 2.7.3.2) in guinea-pig smooth, cardiac and skeletal muscles as well as in brain were analyzed by cellulose acetate electrophoresis and FPLC gel permeation chromatography. In crude tissue extracts of smooth muscles brain type BB-CK and the hybrid form MB-CK were detected, but in en...

journal_title：FEBS letters

authors： Ishida Y,Wyss M,Hemmer W,Wallimann T

更新日期：1991-05-20 00:00:00

abstract：:Major myelin proteolipid (MMPL, also called PLP) and DM-20 are the two major intrinsic membrane proteins of CNS myelin. A specific immunological probe was obtained for MMPL by raising antibodies against the synthetic tridecapeptide 117-129 of MMPL. Antibodies against this peptide reacted with the MMPL but did not cros...

journal_title：FEBS letters

authors： Trifilieff E,Luu B,Nussbaum JL,Roussel G,Espinosa de los Monteros A,Sabatier JM,Van Rietschoten J

更新日期：1986-03-31 00:00:00

abstract：:We have previously shown that Rap1 as well as its guanine nucleotide exchange factor Epac1 increases cell-cell junction formation. Here, we show that activation of Epac1 with the exchange protein directly activated by cAMP (Epac)-specific cAMP analog 8CPT-2'O-Me-cAMP (007) resulted in a tightening of the junctions and...

journal_title：FEBS letters

authors： Kooistra MR,Corada M,Dejana E,Bos JL

更新日期：2005-09-12 00:00:00

abstract：:Epiregulin (EPR), a novel member of epidermal growth factor (EGF) family, is a ligand for ErbB-1 and ErbB-4 receptors. The binding affinity of EPR for the receptors is lower than those of other EGF-family ligands. The solution structure of EPR was determined using two-dimensional nuclear magnetic resonance spectroscop...

journal_title：FEBS letters

authors： Sato K,Nakamura T,Mizuguchi M,Miura K,Tada M,Aizawa T,Gomi T,Miyamoto K,Kawano K

更新日期：2003-10-23 00:00:00

abstract：:The neurogenic gene Drosophila big brain (bib) has a high sequence homology to aquaporin-4. However, its cellular functions in Drosophila neurogenesis have remained elusive. Here we investigated cell adhesion, and the ion and water permeability of Bib. The adhesive function was examined by a cell aggregation assay usi...

journal_title：FEBS letters

authors： Tatsumi K,Tsuji S,Miwa H,Morisaku T,Nuriya M,Orihara M,Kaneko K,Okano H,Yasui M

更新日期：2009-06-18 00:00:00

abstract：:The range of delta-selectivity of linear and cyclic analogues of enkephalin in rat brain was found to be: [D-Pen2, L-Pen5] enkephalin (DPLPE) greater than [D-Pen2, D-Pen5] enkephalin (DPDPE) greater than [D-Thr2, Leu5] enkephalyl-Thr6 (DTLET) greater than [D-Ser2, Leu5] enkephalyl-Thr6 (DSLET). Saturation experiments ...

journal_title：FEBS letters

authors： Delay-Goyet P,Zajac JM,Rigaudy P,Foucaud B,Roques BP

更新日期：1985-04-22 00:00:00

abstract：:Histone acetyltransferase activity was measured in isolated nuclei during the synchronous cell cycle of the myxomycete Physarum polycephalum. Nuclei were incubated with [14C]acetyl-coenzyme A and an excess of exogenous calf thymus histones. The activity is periodic during the cell cycle; it rises during the S-phase to...

journal_title：FEBS letters

authors： Golderer G,Loidl P,Gröbner P

更新日期：1987-10-05 00:00:00

abstract：:All the known members of the mouse Notch receptor family were examined for their biochemical function by interaction with a DNA binding protein RBP-Jkappa. mNotch2, mNotch3 and int3 (= mNotch4) were shown to interact with RBP-Jkappa by the GST-fusion pull down assay and dominant negative competition with Epstein Barr ...

journal_title：FEBS letters

authors： Kato H,Sakai T,Tamura K,Minoguchi S,Shirayoshi Y,Hamada Y,Tsujimoto Y,Honjo T

更新日期：1996-10-21 00:00:00

abstract：:Cav3.2 T-type Ca(2+) channel is required for the activation of calcineurin/NFAT signaling in cardiac hypertrophy. We aimed to investigate how Cav3.2 and calcineurin interact. We found that Ca(2+) and calmodulin modulate the Cav3.2/calcineurin interaction. Calcineurin binding to Cav3.2 decreases the enzyme's phosphatas...

journal_title：FEBS letters

authors： Huang CH,Chen YC,Chen CC

更新日期：2013-06-19 00:00:00

abstract：:The inhibitory effect of actin on protein phosphorylation by three distinct protein kinases (CK-II, A-kinase and MAP-kinase) was examined in vitro. It was found that: (i) actin inhibits the activities of alpha-monomeric CK-II (CK-IIalpha) as well as oligomeric CK-II (alpha2beta2) in a dose-dependent manner, but has no...

journal_title：FEBS letters

authors： Karino A,Tanoue S,Fukuda M,Nakamura T,Ohtsuki K

更新日期：1996-12-02 00:00:00

abstract：:The WWE domain is often identified in proteins associated with ubiquitination or poly-ADP-ribosylation. Structural information about WWE domains has been obtained for the ubiquitination-related proteins, such as Deltex and RNF146, but not yet for the poly-ADP-ribose polymerases (PARPs). Here we determined the solution...

journal_title：FEBS letters

authors： He F,Tsuda K,Takahashi M,Kuwasako K,Terada T,Shirouzu M,Watanabe S,Kigawa T,Kobayashi N,Güntert P,Yokoyama S,Muto Y

更新日期：2012-11-02 00:00:00