The His-probe method: effects of histidine residues introduced into the complementarity-determining regions of antibodies on antigen-antibody interactions at different pH values.

abstract：:Lipid analyses were carried out on transplantable murine Friend leukemia cell tumors, 6 h after intratumoral administration of tumor necrosis factor-alpha (TNF). The levels of the major phospholipid classes were uniformly decreased to about 70% of control values; free fatty acids were increased to about 170%; diacylgl...

journal_title：FEBS letters

authors： Bravo E,Carpinelli G,Proietti E,Belardelli F,Cantafora A,Podo F

更新日期：1990-01-29 00:00:00

abstract：:A cDNA encoding a novel plant type III polyketide synthase (PKS) was cloned from rhubarb (Rheum palmatum). A recombinant enzyme expressed in Escherichia coli accepted acetyl-CoA as a starter, carried out six successive condensations with malonyl-CoA and subsequent cyclization to yield an aromatic heptaketide, aloesone...

journal_title：FEBS letters

authors： Abe I,Utsumi Y,Oguro S,Noguchi H

更新日期：2004-03-26 00:00:00

abstract：:Bacterial fructosyltransferase enzymes belonging to glycoside hydrolase family 68 (GH68) are not known to require a metal cofactor. Here, we show that Ca2+ ions play an important structural role in the Lactobacillus reuteri 121 levansucrase (Lev) and inulosucrase (Inu) enzymes. Analysis of the Bacillus subtilis Lev 3D...

journal_title：FEBS letters

authors： Ozimek LK,Euverink GJ,van der Maarel MJ,Dijkhuizen L

更新日期：2005-02-14 00:00:00

abstract：:Cell migration over or through the extracellular matrix (ECM) is an integral feature of both physiological and pathological processes. Regulation of the changing cell-ECM interactions involved can be effected by proteolysis and requires strict spatial and temporal targeting of proteinase activity. The versatile use of...

journal_title：FEBS letters

authors： Ellis V,Murphy G

更新日期：2001-09-28 00:00:00

abstract：:We searched for the previously defined uncoupling protein (UCP) signatures [Jezek, P. and Urbánková, E. (2000) IUBMB Life 49, 63-70] in genomes of Drosophila melanogaster, Caenorhabditis elegans, Dictyostelium discoideum, and Arabidopsis thaliana. We identified four UCPs in Drosophila and one in Caenorhabditis or Dict...

journal_title：FEBS letters

authors： Hanák P,Jezek P

更新日期：2001-04-27 00:00:00

abstract：:The inhibitory effects of various nitrogen-containing sugars on porcine kidney trehalase were studied. Validamycin A, validoxylamine A and MDL 25,637 were found to be potent, time-dependent inhibitors of the enzyme in vitro. The validoxylamine A-inhibited enzyme showed slow but reversible reactivation over time (t1/2 ...

journal_title：FEBS letters

authors： Salleh HM,Honek JF

更新日期：1990-03-26 00:00:00

abstract：:Recombinant adipogenesis inhibitory factor (AGIF) was purified to homogeneity from the conditioned medium of COS-1 cells transfected with human AGIF cDNA. The amino-terminal sequence analysis of the mature AGIF revealed that AGIF was produced as a precursor consisting of 199 amino acids and processed into a mature for...

journal_title：FEBS letters

authors： Ohsumi J,Miyadai K,Kawashima I,Ishikawa-Ohsumi H,Sakakibara S,Mita-Honjo K,Takiguchi Y

更新日期：1991-08-19 00:00:00

abstract：:Fluorescence techniques were used to study conformational changes that occur in inactive E. coli 30 S ribosomal subunits during activation by heating in 12 mM Mg2+. Activation is associated with movement of a fluorophore on the 3'-end of 16 S RNA into a less polar environment and towards a probe on the cysteine thiol ...

journal_title：FEBS letters

authors： Odom OW,Stöffler G,Hardesty B

更新日期：1984-07-23 00:00:00

abstract：:Rho-associated kinase 2 (Rock2) is known to promote tumorigenesis in hepatocellular carcinoma (HCC). CCAAT/enhancer-binding protein delta (CEBPD) functions as a tumor suppressor. In this study, we found that the expression of Rock2 and CEBPD are inversely correlated. Knockdown of Rock2 increased CEBPD expression and i...

journal_title：FEBS letters

authors： Li M,Zhou W,Yuan R,Chen L,Liu T,Huang D,Hao L,Xie Y,Shao J

更新日期：2015-04-13 00:00:00

abstract：:The sequence of seminalplasmin, a basic antimicrobial and transcription-inhibitory protein from bovine seminal plasma, has been determined using an automated sequenator. This sequence is slightly different from that reported earlier by Theil and Scheit [(1983) EMBO J. 2, 1159-1163] and identical with that of caltrin, ...

journal_title：FEBS letters

authors： Sitaram N,Kumari VK,Bhargava PM

更新日期：1986-06-09 00:00:00

abstract：:Evidence has previously suggested that cellobiose:quinone oxidoreductase (CBQ) in cellulolytic cultures of Phanerochaete chrysosporium might be produced from cellobiose oxidase (CBO) by proteolytic cleavage. This study demonstrates that the ratio of CBO activity to (CBO + CBQ) activity declines with decreasing culture...

journal_title：FEBS letters

authors： Habu N,Samejima M,Dean JF,Eriksson KE

更新日期：1993-07-26 00:00:00

abstract：:Gamma-aminobutyric acid type A (GABAA) receptor beta1 (gabrb1), a subunit of GABAA receptors involved in inhibitory effects on neurotransmission, was found to associate with the formation of protease-resistant prion protein in prion-infected neuroblastoma cells. Silencing of gabrb1 gene expression significantly decrea...

journal_title：FEBS letters

authors： Kimura T,Ishikawa K,Sakasegawa Y,Teruya K,Sata T,Schätzl H,Doh-ura K

更新日期：2010-03-19 00:00:00

abstract：:Bovine globin has been incubated with mice peritoneal macrophages in order to study its hydrolysis by lysosomal enzymes, among which chiefly cathepsin D. Analysis of resulting peptides, by reversed-phase high-performance liquid chromatography (RP-HPLC), showed the release of a bioactive peptide, VV-hemorphin-7. When a...

journal_title：FEBS letters

authors： Dagouassat N,Garreau I,Sannier F,Zhao Q,Piot JM

更新日期：1996-03-11 00:00:00

abstract：:In higher eukaryotes, nine aminoacyl-tRNA synthetases form a multienzyme complex also comprising the three auxiliary proteins p18, p38 and p43, of apparent molecular masses of 18, 38 and 43 kDa. The function of these proteins, invariably found associated to the synthetase components of the complex, is unknown. In orde...

journal_title：FEBS letters

authors： Quevillon S,Mirande M

更新日期：1996-10-14 00:00:00

abstract：:Homocysteine thiolactone is a product of an error-editing reaction, catalyzed by Escherichia coli and Saccharomyces cerevisiae methionyl-tRNA synthetases, which prevents incorporation of homocysteine into tRNA and protein both in vitro and in vivo. Here, homocysteine thiolactone is also shown to be synthesized by cult...

journal_title：FEBS letters

authors： Jakubowski H,Goldman E

更新日期：1993-02-15 00:00:00

abstract：:Over the past several years, the study of a hereditary tumor syndrome, tuberous sclerosis complex (TSC), has shed light on the regulation of cellular proliferation and growth. TSC is an autosomal dominant disorder that is due to inactivating mutations in TSC1 or TSC2 and characterized by benign tumors (hamartomas) inv...

journal_title：FEBS letters

authors： Han JM,Sahin M

更新日期：2011-04-06 00:00:00

abstract：:We have purified a form of protein phosphatase 1 (PP1) from HeLa cell nuclei, in which the phosphatase is complexed to a regulatory subunit termed p99. We report here the cloning and characterisation of the p99 component. p99 mRNA is widely expressed in human tissues and immunofluorescence analysis with anti-p99 antib...

journal_title：FEBS letters

authors： Kreivi JP,Trinkle-Mulcahy L,Lyon CE,Morrice NA,Cohen P,Lamond AI

更新日期：1997-12-22 00:00:00

abstract：:Treatment of human amniotic cells (UAC) with human interferon-alpha (Hu-IFN alpha) or phorbol myristate acetate (PMA) resulted in translocation of protein kinase C (PK-C) activity from the cytosol fraction to that of the membranes. Analysis of 32P incorporation into phospholipid fractions and studies of alterations in...

journal_title：FEBS letters

authors： Premecz G,Markovits A,Bagi G,Farkas T,Földes I

更新日期：1989-06-05 00:00:00

abstract：:Frame shift mutations of the polyglutamine binding protein-1 (PQBP1) gene lead to total or partial truncation of the C-terminal domain (CTD) and cause mental retardation in human patients. Interestingly, normal Drosophila homologue of PQBP-1 lacks CTD. As a model to analyze the molecular network of PQBP-1 affecting in...

journal_title：FEBS letters

authors： Yoshimura N,Horiuchi D,Shibata M,Saitoe M,Qi ML,Okazawa H

更新日期：2006-04-17 00:00:00

abstract：:Amiloride, a specific inhibitor for the Na(+)-driven flagellar motors of alkalophilic Bacillus, is known to inhibit secondarily the growth of alkalophiles. The motility of a marine Vibrio, V. alginolyticus, was almost completely inhibited by 2 mM amiloride either at pH 7.0 or 8.5. We found that this concentration of a...

journal_title：FEBS letters

authors： Atsumi T,Maekawa Y,Tokuda H,Imae Y

更新日期：1992-12-14 00:00:00

abstract：:In the central nervous system, synaptic signal transduction depends on the regulation of neurotransmitter receptors by interacting proteins. Here, we searched for proteins interacting with two metabotropic glutamate receptor type 8 isoforms (mGlu8a and mGlu8b) and identified RanBPM. RanBPM is expressed in several brai...

journal_title：FEBS letters

authors： Seebahn A,Rose M,Enz R

更新日期：2008-07-09 00:00:00

abstract：:Photon correlation spectroscopy demonstrated for the first time that co-purified meningococcal TbpA+B form a complex in solution. This structure bound hTf and the resultant species underwent partial dissociation after exposure to additional hTf or following prolonged incubation. Purified TbpA and TbpB had similar appa...

journal_title：FEBS letters

authors： Boulton IC,Gorringe AR,Carr RJ,Gorinsky B,Joannou CL,Evans RW

更新日期：1997-09-08 00:00:00

abstract：:We report for the first time the identification of 25 microRNAs from tissues originating from chicken embryo and adult chicken. Most of the cloned microRNAs are expressed in both adult chickens and chicken embryos. Fourteen were identified without any prior prediction. One microRNA, miR-757, is thought to be chicken-s...

journal_title：FEBS letters

authors： Xu H,Wang X,Du Z,Li N

更新日期：2006-06-26 00:00:00

abstract：:Choroidal melanoma (CM) remains the most prevalent form of intraocular malignancy, and the prognosis of affected patients is poor. While the E3 ubiquitin ligase TRAF-interacting protein (TRIP) is known to play key regulatory roles in multiple diseases, its relevance in CM remains uncertain. In the present study, we fo...

journal_title：FEBS letters

authors： Wei C,Zhao X,Wang L,Zhang H

更新日期：2020-10-01 00:00:00

abstract：:Molecular dynamics simulations have been performed on the intrinsically disordered 39-residue N-terminal transactivation domain of p53 (p53(1-39)). Simulations not only revealed that p53(1-39) is natively compact, but also possesses a folded structure. Furthermore, leucine-rich hydrophobic clusters were found to play ...

journal_title：FEBS letters

authors： Espinoza-Fonseca LM

更新日期：2009-02-04 00:00:00

abstract：:Five mouse and human leptin receptors (Ob-R) have recently been identified, a long isoform (Ob-Rb), preferentially expressed in hypothalamus, and 4 short isoforms, Ob-Ra, Ob-Rc, Ob-Rd, and Ob-Re. We have identified a new short isoform in the rat, r-OB-Rf, with 6 C-terminal amino acids and a 3' untranslated region with...

journal_title：FEBS letters

authors： Wang MY,Zhou YT,Newgard CB,Unger RH

更新日期：1996-08-26 00:00:00

abstract：:We examined the expression of peroxisome proliferator-activated receptors (PPARs) and the role of PPARs in cytokine production in mouse bone marrow-derived mast cells (mBMMCs). mBMMCs expressed PPARbeta strongly and gamma slightly, but not alpha. Activation of mBMMCs with antigen or calcium ionophore resulted in the i...

journal_title：FEBS letters

authors： Sugiyama H,Nonaka T,Kishimoto T,Komoriya K,Tsuji K,Nakahata T

更新日期：2000-02-11 00:00:00

abstract：:The involvement of reactive oxygen species in Ca(2+)-induced mitochondrial membrane permeabilization and cell viability was studied using yeast cells in which the thioredoxin peroxidase (TPx) gene was disrupted and/or catalase was inhibited by 3-amino-1,2, 4-triazole (ATZ) treatment. Wild-type Saccharomyces cerevisiae...

journal_title：FEBS letters

authors： Kowaltowski AJ,Vercesi AE,Rhee SG,Netto LE

更新日期：2000-05-12 00:00:00

abstract：:Differentiation of glia (astrocytes and oligodendrocytes) in Drosophila requires the gene glial cells missing (gcm), which controls lineage determination. In the absence of gcm, neuroglia progenitors exclusively differentiate into neurons, instead of into both neurons and glia. In contrast, ectopic overexpression of g...

journal_title：FEBS letters

authors： Altshuller Y,Copeland NG,Gilbert DJ,Jenkins NA,Frohman MA

更新日期：1996-09-16 00:00:00

abstract：:Activation of T lymphocytes results in an increase in intracellular Ca2+ due in large part to influx of extracellular Ca2+. Using the patch clamp technique, an inward current in Jurkat T lymphocytes was observed upon depolarization from a holding potential of -90 mV but not from -60 mV. This whole-cell current was ins...

journal_title：FEBS letters

authors： Densmore JJ,Szabo G,Gray LS

更新日期：1992-11-09 00:00:00