Abstract:
:Bovine globin has been incubated with mice peritoneal macrophages in order to study its hydrolysis by lysosomal enzymes, among which chiefly cathepsin D. Analysis of resulting peptides, by reversed-phase high-performance liquid chromatography (RP-HPLC), showed the release of a bioactive peptide, VV-hemorphin-7. When a carboxyl proteinase inhibitor such as pepstatin A was added, no hemorphin was generated. Our results clearly demonstrated that VV-hemorphin-7 generation was principally due to cathepsin D. This study allowed us to hypothesize a possible pathway for in vivo hemorphins appearance from globin catabolism by macrophages.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Dagouassat N,Garreau I,Sannier F,Zhao Q,Piot JMdoi
10.1016/0014-5793(96)00144-5subject
Has Abstractpub_date
1996-03-11 00:00:00pages
37-42issue
1-2eissn
0014-5793issn
1873-3468journal_volume
382pub_type
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