Generation of VV-hemorphin-7 from globin by peritoneal macrophages.


:Bovine globin has been incubated with mice peritoneal macrophages in order to study its hydrolysis by lysosomal enzymes, among which chiefly cathepsin D. Analysis of resulting peptides, by reversed-phase high-performance liquid chromatography (RP-HPLC), showed the release of a bioactive peptide, VV-hemorphin-7. When a carboxyl proteinase inhibitor such as pepstatin A was added, no hemorphin was generated. Our results clearly demonstrated that VV-hemorphin-7 generation was principally due to cathepsin D. This study allowed us to hypothesize a possible pathway for in vivo hemorphins appearance from globin catabolism by macrophages.




FEBS letters


Dagouassat N,Garreau I,Sannier F,Zhao Q,Piot JM




Has Abstract


1996-03-11 00:00:00












  • Fluorescence properties of the Ca2+,Mg2(+)-ATPase protein of sarcoplasmic reticulum labeled with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole.

    abstract::The fluorescence intensity of the Ca2+,Mg2(+)-ATPase protein of rabbit skeletal sarcoplasmic reticulum that incorporated about 2 mol of 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) was enhanced at high MgATP concentrations with or without 50 microM calcium. The observed enhancement indicates that the fluorophore, ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bailin G,Huang JR

    更新日期:1990-01-01 00:00:00

  • Sulphur islands in the Escherichia coli genome: markers of the cell's architecture?

    abstract::Two highly contrasted images depict genomes: at first sight, genes appear to be distributed randomly along the chromosome. In contrast, their organisation into operons (or pathogenicity islands) suggests that, at least locally, related functions are in physical proximity. Analysis of the codon usage bias in orthologou...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Rocha EP,Sekowska A,Danchin A

    更新日期:2000-06-30 00:00:00

  • Changes in the proportions of histone H1 subtypes in brain cortical neurons.

    abstract::Histone H1(0) is found in tissues with little or no cellular proliferation and has been shown to accumulate during cellular terminal differentiation. Two subtypes of H1(0), H1 a and H1 b, are present in any tissue where the protein has been detected. We report here the first evidence of an age-dependent change in the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Piña B,Suau P

    更新日期:1987-01-05 00:00:00

  • Copper in the silk formation process of Bombyx mori silkworm.

    abstract::Evidence is presented here that cupric ions play a part in the natural spinning of Bombyx mori silk. Proton induced X-ray emission studies revealed that the copper content increased from the posterior part to the anterior part of silk gland, and then further increased in the silk fiber. Spectrophotometric analysis dem...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zhou L,Chen X,Shao Z,Zhou P,Knight DP,Vollrath F

    更新日期:2003-11-20 00:00:00

  • Activation of the WT1 tumor suppressor gene promoter by Pea3.

    abstract::Gene array profiling of RNA from cells engineered to express a dominant-negative version of the ETS family member transcription factor Pea3 (polyomavirus enhancer activator 3) identified WT1 as a candidate downstream gene. Given the co-expression of WT1 and Pea3 in developing kidney and breast tissue undergoing mesenc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Discenza MT,Vaz D,Hassell JA,Pelletier J

    更新日期:2004-02-27 00:00:00

  • Rank products: a simple, yet powerful, new method to detect differentially regulated genes in replicated microarray experiments.

    abstract::One of the main objectives in the analysis of microarray experiments is the identification of genes that are differentially expressed under two experimental conditions. This task is complicated by the noisiness of the data and the large number of genes that are examined simultaneously. Here, we present a novel techniq...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Breitling R,Armengaud P,Amtmann A,Herzyk P

    更新日期:2004-08-27 00:00:00

  • Nuclear functions in space and time: gene expression in a dynamic, constrained environment.

    abstract::All eukaryotic cells enclose their genome within a dedicated, membrane-bound organelle termed the nucleus, which functions to partition gene transcription from sites of protein translation in the cytoplasm. Despite a great deal of research effort, basic questions about chromosome structure and gene expression mechanis...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Trinkle-Mulcahy L,Lamond AI

    更新日期:2008-06-18 00:00:00

  • The KDEL receptor: new functions for an old protein.

    abstract::The KDEL receptor is a seven-transmembrane-domain protein that was first described about 20 years ago. Its well-known function is to retrotransport chaperones from the Golgi complex to the endoplasmic reticulum. Recent studies, however, have suggested that the KDEL receptor has additional functions. Indeed, we have de...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Capitani M,Sallese M

    更新日期:2009-12-03 00:00:00

  • Sodium butyrate suppresses apoptosis in human Burkitt lymphomas and murine plasmacytomas bearing c-myc translocations.

    abstract::We report that sodium butyrate, a natural product of fiber degradation by colonic bacteria, markedly suppresses c-Myc-mediated apoptosis induced in murine plasmacytomas and human Burkitt lymphomas by growth factor deprivation, but not in cell lines devoid of c-myc translocations. Attenuation of cell death is associate...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Alexandrov I,Romanova L,Mushinski F,Nordan R

    更新日期:1998-08-28 00:00:00

  • MDR-1 gene expression is a minor factor in determining the multidrug resistance phenotype of MCF7/ADR and KB-V1 cells.

    abstract::The relevance of MDR-1 gene expression to the multidrug resistance phenotype was investigated. Drug-resistant cells, KB-V1 and MCF7/ADR, constantly expressed mRNA of the MDR-1 gene and were more resistant to vinblastine and adriamycin than drug-sensitive cells, KB-3-1 and MCF7. The drug efflux rate of KB-V1 was the sa...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kim HM,Oh GT,Hong DH,Kim MS,Kang JS,Park SM,Han SB

    更新日期:1997-07-21 00:00:00

  • HNF1 alpha activates the aminopeptidase N promoter in intestinal (Caco-2) cells.

    abstract::The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco-2 cell-line. Aminopeptidase N promoter activity in Caco-2 cells depends on the HNF1 element (positions -85 to -58) and co-transfection with an HNF1 alpha expression vector demonstrates a direct activation...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Olsen J,Laustsen L,Troelsen J

    更新日期:1994-04-11 00:00:00

  • FAK inhibitors induce cell multinucleation and dramatically increase pro-tumoral cytokine expression in RAW 264.7 macrophages.

    abstract::Macrophages are abundant in the tumor microenvironment. They are highly plastic and able to acquire pro-tumoral phenotypes in response to microenvironmental stimuli. When we treated RAW 264.7 macrophages with inhibitors of various oncogenic pathways, we found that the focal adhesion kinase (FAK) inhibitors PF573228 an...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: He X,Chen X,Li B,Ji J,Chen S

    更新日期:2017-12-01 00:00:00

  • Low-density lipoproteins interact with liposome-binding sites on the cell surface.

    abstract::Under physiological conditions significant amounts of low-density lipoprotein LDL particles ar taken up by cells independently of specific high-affinity LDL receptors (apo-B receptors). Previously it was established that some cells contain surface sites capable of binding liposomes. We proposed that liposome-binding s...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Galkina SI,Ivanov VV,Preobrazhensky SN,Margolis LB,Bergelson LD

    更新日期:1991-08-05 00:00:00

  • Conformational change of 50 S ribosomes on enzymatic binding of phenylalanyl-tRNA.

    abstract::Tight couple 70 S ribosomes are converted to loose couple ones on enzymatic binding of phenylalanyl-tRNA. Enzymatic binding at 0 degree C as well as nonenzymatic binding does not lead to any change. Further, no change takes place when the P site is occupied by N-acetylphenylalanyl-tRNA. Loose couple 70 S ribosomes are...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Srivastava S,Burma DP

    更新日期:1985-07-01 00:00:00

  • A key role of nuclear factor Y in the refeeding response of fatty acid synthase in adipocytes.

    abstract::Fatty acid synthase (Fasn) is a key component of energy metabolism that is dynamically induced by food intake. Although extensive studies have revealed a number of transcription factors involved in the fasting/refeeding transition of Fasn expression in hepatocytes, much less evidence is available for adipocytes. Using...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nishi-Tatsumi M,Yahagi N,Takeuchi Y,Toya N,Takarada A,Murayama Y,Aita Y,Sawada Y,Piao X,Oya Y,Shikama A,Masuda Y,Kubota M,Izumida Y,Matsuzaka T,Nakagawa Y,Sekiya M,Iizuka Y,Kawakami Y,Kadowaki T,Yamada N,Shimano

    更新日期:2017-04-01 00:00:00

  • Stimulation and inhibition of human platelet membrane high-affinity GTPase by neomycin.

    abstract::The effect of the inositol phospholipid-binding antibiotic neomycin was studied on high-affinity GTPase in human platelet membranes. At low concentrations (up to 1 mM), neomycin by itself stimulated a high-affinity GTPase. This GTPase stimulation was additive with that caused by the hormonal factors, prostaglandin E1 ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Herrmann E,Jakobs KH

    更新日期:1988-02-29 00:00:00

  • Role of phospholipid in the low affinity reactions between cytochrome c and cytochrome oxidase.

    abstract::The steady-state oxidation of ferrocytochrome c by cytochrome oxidase monitored spectrophotometrically showed that: (1) the kinetics were strictly biphasic with purified enzyme, while mitochondrial membrane-bound enzyme exhibited multiphasic kinetics with extended low affinity phases; (2) the TNmax for the highest aff...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Speck SH,Neu CA,Swanson MS,Margoliash E

    更新日期:1983-12-12 00:00:00

  • Dynamic re-distribution of protein kinase D (PKD) as revealed by a GFP-PKD fusion protein: dissociation from PKD activation.

    abstract::Protein kinase D (PKD)/protein kinase Cmicro (PKCmicro, a serine/threonine protein kinase with distinct structural and enzymological properties, is rapidly activated in intact cells via PKC. The amino-terminal region of PKD contains a cysteine-rich domain (CRD) that directly binds phorbol esters with a high affinity. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Matthews S,Iglesias T,Cantrell D,Rozengurt E

    更新日期:1999-09-03 00:00:00

  • Modulation of EWS/WT1 activity by the v-Src protein tyrosine kinase.

    abstract::Desmoplastic small round cell tumor (DSRCT) is a malignant human cancer that is associated with a specific t(11;22) chromosome translocation, where 265 amino acids from the EWS amino-terminus are fused to the DNA binding domain of the WT1 tumor suppressor gene. We have noticed the presence of several SH3 interacting d...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kim J,Lee JM,Branton PE,Pelletier J

    更新日期:2000-06-02 00:00:00

  • Selective inhibition of divergent seryl-tRNA synthetases by serine analogues.

    abstract::Seryl-tRNA synthetases (SerRSs) fall into two distinct evolutionary groups of enzymes, bacterial and methanogenic. These two types of SerRSs display only minimal sequence similarity, primarily within the class II conserved motifs, and possess distinct modes of tRNA(Ser) recognition. In order to determine whether the t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ahel D,Slade D,Mocibob M,Söll D,Weygand-Durasevic I

    更新日期:2005-08-15 00:00:00

  • NMR analysis of secondary structure and dynamics of a recombinant peptide from the N-terminal region of human erythroid alpha-spectrin.

    abstract::We have studied the nuclear magnetic resonance solution secondary structure of the N-terminal region in human erythroid alpha-spectrin using a recombinant model peptide of alpha-spectrin consisting of residues 1-156. Pulsed field gradient diffusion coefficient measurements show that the model peptide exists as a monom...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Park S,Johnson ME,Fung LW

    更新日期:2000-11-17 00:00:00

  • Temperature effects on the presteady-state and transport-associated currents of GABA cotransporter rGAT1.

    abstract::The effects of temperature on the gamma-aminobutyric acid (GABA) uptake and on the presteady-state and transport-associated currents of the GABA cotransporter, rat gamma-aminobutyric acid transporter 1 (rGAT1), have been studied using heterologous oocyte expression and voltage-clamp. Increasing temperature from 15 to ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Binda F,Bossi E,Giovannardi S,Forlani G,Peres A

    更新日期:2002-02-13 00:00:00

  • Characterization of a 41 kDa photoinhibition adduct in isolated photosystem II reaction centres.

    abstract::When isolated reaction centres of photosystem II are subjected to photoinhibitory illumination, a 41 kDa SDS-PAGE band is observed under all experimental conditions. The same band is also found, together with lower molecular weight fragments of the D1 protein, in whole thylakoids and in all PSII sub-particles investig...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Barbato R,Friso G,Rigoni F,Frizzo A,Giacometti GM

    更新日期:1992-09-07 00:00:00

  • Antibodies directed against guanylic acid and guanosine are nucleolus specific probes.

    abstract::Purified antibodies against guanylic acid and guanosine binding to RNA at guanosine residues were used to probe human lymphocyte preparations by indirect immunofluorescence. Neither antibody gave any banding pattern with metaphase chromosomes but both showed binding to specific sites in the interphase nuclei. Evidence...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Iswari S,Krishnamurthy DS,Jacob TM

    更新日期:1985-09-23 00:00:00

  • A sweet code for glycoprotein folding.

    abstract::Glycoprotein synthesis is initiated in the endoplasmic reticulum (ER) lumen upon transfer of a glycan (Glc3Man9GlcNAc2) from a lipid derivative to Asn residues (N-glycosylation). N-Glycan-dependent quality control of glycoprotein folding in the ER prevents exit to Golgi of folding intermediates, irreparably misfolded ...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Caramelo JJ,Parodi AJ

    更新日期:2015-11-14 00:00:00

  • Co-localization of CD3 and prion protein in Jurkat lymphocytes after hypothermal stimulation.

    abstract::While long-term effects of temperature treatment in respect of, e.g., gene-expression and cellular function have already been studied in some detail, nothing is known on the physiological responses of lymphocytes during short-term hypothermal shifts. In this report, we characterized the effects of such a stimulation u...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wurm S,Paar C,Sonnleitner A,Sonnleitner M,Höglinger O,Romanin C,Wechselberger C

    更新日期:2004-05-21 00:00:00

  • The evolution of BIR domain and its containing proteins.

    abstract::BIR domain and its containing proteins play critical roles in cell apoptosis and cell division. Here several lines of novelty were revealed based on a comprehensive evolutionary analysis of BIR domains in 11 representative organisms. First, the type II BIR domains in Survivin and Bruce showed more conservation compare...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cao L,Wang Z,Yang X,Xie L,Yu L

    更新日期:2008-11-12 00:00:00

  • Postnatal developmental profiles of filamentous actin and of 200 kDa neurofilament polypeptide in the visual cortex of light- and dark-reared rats and their relationship to critical period plasticity.

    abstract::The concept of critical period plasticity in rat visual cortex has been studied in terms of changes in the level of filamentous actin and of the 200 kDa neurofilament polypeptide. Our results suggest that the postnatal developmental profile of filamentous actin is affected by visual experience, as a consequence of eye...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nona SN,Trowell SC,Cronly-Dillon JR

    更新日期:1985-07-01 00:00:00

  • Copolymers of N-isopropylacrylamide can trigger pH sensitivity to stable liposomes.

    abstract::Stable liposomes were rendered pH-sensitive by complexation to a polymer that undergoes marked temperature- and pH-dependent water solubility changes. The N-isopropylacrylamide-methacrylic acid copolymer was prepared with or without octadecyl acrylate. At pH below the phase transition of the polymer, egg phosphatidylc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Meyer O,Papahadjopoulos D,Leroux JC

    更新日期:1998-01-02 00:00:00

  • Glycogen synthase from human and bovine polymorphonuclear leukocyte. Immunochemical characterization and comparison to glycogen synthase from rat and rabbit muscle and liver cells.

    abstract::Glycogen synthase from human and bovine polymorphonuclear leukocytes was purified to homogeneity. Rabbit antisera were raised against the two glycogen synthases and used for immunochemical analysis. Western blotting analysis showed that the subunit of glycogen synthase in crude homogenates of human and bovine leukocyt...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rasmussen LH,Juhl H,Esmann V,Petersen HU

    更新日期:1988-03-28 00:00:00