Generation of VV-hemorphin-7 from globin by peritoneal macrophages.


:Bovine globin has been incubated with mice peritoneal macrophages in order to study its hydrolysis by lysosomal enzymes, among which chiefly cathepsin D. Analysis of resulting peptides, by reversed-phase high-performance liquid chromatography (RP-HPLC), showed the release of a bioactive peptide, VV-hemorphin-7. When a carboxyl proteinase inhibitor such as pepstatin A was added, no hemorphin was generated. Our results clearly demonstrated that VV-hemorphin-7 generation was principally due to cathepsin D. This study allowed us to hypothesize a possible pathway for in vivo hemorphins appearance from globin catabolism by macrophages.




FEBS letters


Dagouassat N,Garreau I,Sannier F,Zhao Q,Piot JM




Has Abstract


1996-03-11 00:00:00












  • Quantitative analysis of the effect of cell type and cellular differentiation on protective antigen binding to human target cells.

    abstract::We quantitatively measured protective antigen (PA) binding to human cells targeted by anthrax lethal toxin (LT). Affinities were less than 50 nM for all cells, but differentiated cells (macrophages and neutrophils) had significantly increased PA binding and endothelial cells demonstrated the most binding. Combined wit...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Deshpande A,Hammon RJ,Sanders CK,Graves SW

    更新日期:2006-07-24 00:00:00

  • Human interferon-gamma lacking 23 COOH-terminal amino acids is biologically active.

    abstract::We constructed five mutated cDNAs encoding human interferon-gamma (IFN-gamma) derivatives lacking 19-23 COOH-terminal residues and expressed them in Escherichia coli. All the derivatives were purified to homogeneity. They showed substantially the same order of antiviral activity in vitro as the intact molecule, and be...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sakaguchi M,Honda S,Ozawa M,Nishimura O

    更新日期:1988-03-28 00:00:00

  • Functional identification of ATP-driven Ca2+ pump in the peribacteroid membrane of broad bean root nodules.

    abstract::A Ca2+ indicator arsenazo III was used to demonstrate calcium uptake activity of symbiosomes and the peribacteroid membrane (PBM) vesicles isolated from broad bean root nodules and placed in the medium containing ATP and Mg2+ ions. This process was shown to be rapidly stopped by vanadate, completely reversed in the pr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Andreev IM,Dubrovo PN,Krylova VV,Izmailov SF

    更新日期:1999-03-19 00:00:00

  • Pore mutations affecting tetrameric assembly and functioning of the potassium channel KcsA from Streptomyces lividans.

    abstract::Designed mutations within the Streptomyces lividans kcsA gene resulted in a set of mutant proteins, which were characterized in respect to their assembly and channel activities. (i) The amino acid residue leucine 81 located at the external side of KcsA was found to be exchangeable by a cysteine residue without affecti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Splitt H,Meuser D,Borovok I,Betzler M,Schrempf H

    更新日期:2000-04-21 00:00:00

  • Epidermal growth factor stimulates hydrolysis of phosphatidylinositol 4,5-bisphosphate, generation of diacylglycerol and exocytosis in mouse spermatozoa.

    abstract::Mouse spermatozoa stimulated with epidermal growth factor (EGF) or zona pellucida (ZP) experienced phosphatidylinositol 4,5-bisphosphate hydrolysis, diacylglycerol (DAG) generation and acrosomal exocytosis. The agonists showed additive effects but the action of EGF is likely to be mediated by a distinct receptor becau...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Murase T,Roldan ER

    更新日期:1995-03-06 00:00:00

  • The cosubstrate NADP(H) protects lysine 601 in the porcine NADPH-cytochrome P-450 reductase against pyridoxylation.

    abstract::Lys601 in NADPH-cytochrome P-450 reductase is modified by reductive alkylation with pyridoxal 5'-phosphate (pyridoxylation). Lys601 is protected against modification by the cosubstrate NADP(H). ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Müller K,Linder D,Lumper L

    更新日期:1990-01-29 00:00:00

  • Studies on the primary sequence requirements for PKC-alpha, -beta 1 and -gamma peptide substrates.

    abstract::The substrate specificity of purified PKC-alpha, -beta and -gamma has been investigated. A series of synthetic peptides based upon the sequence surrounding serine-7 in glycogen synthase were generated and used to determine the basic residue requirements of these PKC isotypes. While PKC-alpha and -beta are indistinguis...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Marais RM,Nguyen O,Woodgett JR,Parker PJ

    更新日期:1990-12-17 00:00:00

  • Regulation of tau exon 10 splicing by a double stem-loop structure in mouse intron 10.

    abstract::Tau exon 10 (E10) splicing is a crucial event in its developmental change of tau isoform and tauopathy. To investigate the splicing mechanism, we isolated and compared mouse tau genomic sequence with human sequence. We identified a new element in mouse intron 10 (I10) to suppress E10 splicing, which was located just a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yamashita T,Tomiyama T,Li Q,Numata H,Mori H

    更新日期:2005-01-03 00:00:00

  • Two different B-type creatine kinase subunits dimerize in a tissue-specific manner.

    abstract::Brain-type creatine kinase B-CK (EC was purified from several chicken tissues, e.g. cardiac muscle, brain, gizzard and retina. Two major monomeric chicken B-CK subunits, designated Bb (basic) and Ba (acidic), which differ in isoelectric point, were separated by chromatofocusing in the presence of 8 M urea on ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Quest AF,Eppenberger HM,Wallimann T

    更新日期:1990-03-26 00:00:00

  • Adiponectin deficiency suppresses ABCA1 expression and ApoA-I synthesis in the liver.

    abstract::Plasma high density lipoprotein (HDL)-cholesterol levels are inversely correlated with the incidence of cardiovascular diseases. HDL is mainly assembled in the liver through the ATP-binding cassette transporter (ABCA1) pathway. In humans, plasma HDL-cholesterol levels are positively correlated with plasma adiponectin ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Oku H,Matsuura F,Koseki M,Sandoval JC,Yuasa-Kawase M,Tsubakio-Yamamoto K,Masuda D,Maeda N,Ohama T,Ishigami M,Nishida M,Hirano K,Kihara S,Hori M,Shimomura I,Yamashita S

    更新日期:2007-10-30 00:00:00

  • Identification and expression-profiling of Xenopus tropicalis miRNAs including plant miRNA-like RNAs at metamorphosis.

    abstract::Small RNAs (miR159-like RNAs) identical to some plant miR159s were found in Xenopus tropicalis miRNA cDNA libraries (30 miRNA families consisting of 75 unique sequences). Preferential expression of this RNA species was found in neural tissues during development. A sequence matching to this RNA species was not found in...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hikosaka A,Takaya K,Jinno M,Kawahara A

    更新日期:2007-06-26 00:00:00

  • Greater diversity than previously thought of chromaffin cell Ca2+ channels, derived from mRNA identification studies.

    abstract::Using reverse transcription followed by PCR amplification (RT-PCR), we have identified multiple messenger RNAs encoding for the neuronal pore-forming Ca(2+) channel subunits alpha(1A) (P/Q channel), alpha(1B) (N channel), alpha(1D) (neuronal/endocrine L channel), alpha(1E) (R channel), alpha(1G-H) (T channel) and alph...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: García-Palomero E,Cuchillo-Ibáñez I,García AG,Renart J,Albillos A,Montiel C

    更新日期:2000-09-22 00:00:00

  • Site-specific phosphorylation of villin remodels the actin cytoskeleton to regulate Sendai viral glycoprotein-mediated membrane fusion.

    abstract::Connivance of cellular factors during virus-host cell membrane fusion is poorly understood. We have recently shown that cellular villin plays an important role during membrane fusion of reconstituted Sendai virosomes with hepatocytes. Here, we employed villin-null Chinese Hamster Ovary (CHO) cells, where villin expres...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chandra S,Kumar M,Sharma NR,Sarkar DP

    更新日期:2019-08-01 00:00:00

  • Antiferritin single-chain antibody: a functional protein with incomplete folding?

    abstract::The pET(scF11) plasmid was constructed comprising the gene of a single-chain antibody against human ferritin. This plasmid encodes the leader peptide pelB followed by the heavy chain variable V(H) domain, (Gly4Ser)3 linker peptide, and light chain variable V(L) domain. The correctly processed scF11 antibody was expres...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Martsev SP,Kravchuk ZI,Chumanevich AA,Vlasov AP,Dubnovitsky AP,Bespalov IA,Arosio P,Deyev SM

    更新日期:1998-12-28 00:00:00

  • Determination of the vitronectin binding site on plasminogen activator inhibitor 1 (PAI-1).

    abstract::Vitronectin is the carrier protein of plasminogen activator inhibitor 1 (PAI-1). We used a well-characterized panel of anti-human PAI-1 monoclonal antibodies (MoAbs) to localize the vitronectin-binding site on PAI-1. By employing a direct vitronectin/PAI-1 binding assay and two vitronectin-dependent inhibition assays,...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: van Meijer M,Gebbink RK,Preissner KT,Pannekoek H

    更新日期:1994-10-03 00:00:00

  • Extended analyses of the Wnt/beta-catenin pathway: robustness and oscillatory behaviour.

    abstract::The Wnt/beta-catenin signalling pathway is evolutionarily conserved across many species and plays important roles during embryogenesis. The Lee-Heinrich model (to recognize the contributions of R. Heinrich we will refer to the work proposed by Lee et al. [Lee, E., Salic, A., Krüger, R., Heinrich, R., Kirschner, M.W. (...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wawra C,Kühl M,Kestler HA

    更新日期:2007-08-21 00:00:00

  • Oxo-phytodienoic acid (OPDA) is formed on fatty acids esterified to galactolipids after tissue disruption in Arabidopsis thaliana.

    abstract::Biotic and abiotic stress induces the formation of galactolipids esterified with the phytohormones 12-oxo-phytodienoic acid (OPDA) and dinor-oxo-phytodienoic acid (dnOPDA) in Arabidopsis thaliana. The biosynthetic pathways of free (dn)OPDA is well described, but it is unclear how they are incorporated into galactolipi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nilsson AK,Fahlberg P,Ellerström M,Andersson MX

    更新日期:2012-07-30 00:00:00

  • Skp2 induction and phosphorylation is associated with the late G1 phase of proliferating rat hepatocytes.

    abstract::The changes in phosphoproteins purified with the affinity peptide p9CKShs1 were analyzed from extracts of regenerating rat livers in order to define some G1 and G1/S regulations characteristic of mature hepatocytes stimulated to proliferate. We observed a 47 kDa phosphoprotein that occurred first at the end of G1 befo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bilodeau M,Talarmin H,Ilyin G,Rescan C,Glaise D,Cariou S,Loyer P,Guguen-Guillouzo C,Baffet G

    更新日期:1999-06-11 00:00:00

  • Co-localization of CD3 and prion protein in Jurkat lymphocytes after hypothermal stimulation.

    abstract::While long-term effects of temperature treatment in respect of, e.g., gene-expression and cellular function have already been studied in some detail, nothing is known on the physiological responses of lymphocytes during short-term hypothermal shifts. In this report, we characterized the effects of such a stimulation u...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wurm S,Paar C,Sonnleitner A,Sonnleitner M,Höglinger O,Romanin C,Wechselberger C

    更新日期:2004-05-21 00:00:00

  • Determination and cellular localization of adenylyl cyclase isozymes expressed in embryonic chick heart.

    abstract::Mammalian heart has been reported to express AC isozymes (types V and VI) that are inhibited by < microM [Ca2+]; avian heart has been reported to express adenylyl cyclase activity that is inhibited by < microM [Ca2]. We have used reverse transcription polymerase chain reaction (RT-PCR) to determine that type V and VI ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yu HJ,Unnerstall JR,Green RD

    更新日期:1995-10-23 00:00:00

  • A C-terminal proline is required for bioluminescence of the Ca(2+)-binding photoprotein, aequorin.

    abstract::The requirement for a proline residue at the C-terminus of the Ca(2+)-binding photoprotein, aequorin, was investigated by measuring luminescence activities of a series of C-terminal deletion mutants, substitution mutants and an addition mutant. CD spectral measurements of apoaequorin with the C-terminal proline delete...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nomura M,Inouye S,Ohmiya Y,Tsuji FI

    更新日期:1991-12-16 00:00:00

  • The nuclear-encoded 18 kDa (IP) AQDQ subunit of bovine heart complex I is phosphorylated by the mitochondrial cAMP-dependent protein kinase.

    abstract::In bovine heart mitochondria a protein of M(r) 18 kDa, phosphorylated by mtPKA, is associated to the NADH-ubiquinone oxidoreductase in the inner membrane and is present in purified preparation of this complex. The 18 kDa phosphoprotein has now been isolated and sequenced. It is identified as the 18 kDa (IP) AQDQ subun...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Papa S,Sardanelli AM,Cocco T,Speranza F,Scacco SC,Technikova-Dobrova Z

    更新日期:1996-02-05 00:00:00

  • An Escherichia coli cyoE gene homologue in thermophilic Bacillus PS3 encodes a thermotolerant heme O synthase.

    abstract::The cyoE gene of the Escherichia coli bo-type quinol oxidase operon (cyoABCDE) has been previously shown to encode heme O synthase. To demonstrate a catalytic role of a cyoE homologue (the caaE gene) in the gene cluster for caa3-type cytochrome c oxidase of thermophilic Bacillus PS3, we have carried out genetic comple...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Saiki K,Mogi T,Ishizuka M,Anraku Y

    更新日期:1994-09-12 00:00:00

  • Inhibition of ADP-ribosylation suppresses aberrant accumulation of lipidated apolipoprotein B in the endoplasmic reticulum.

    abstract::ApoB-crescent, an endoplasmic reticulum (ER)-lipid droplet amalgamation structure, is a useful marker to indicate aberrant lipidated apolipoprotein B accumulation in the hepatocyte ER. Blockade of the ER-to-Golgi transport by either vesicle transport inhibitors or dominant-negative Arf1 caused a significant increase i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ohsaki Y,Cheng J,Yamairi K,Pan X,Hussain MM,Fujimoto T

    更新日期:2013-11-15 00:00:00

  • Testing the eukaryotic promoters for efficient expression of exogenous genes in chondrocytes and synoviocytes.

    abstract::To identify suitable promoters for expressing exogenous genes in arthritic joints, the constitutive, simian virus 40 (SV 40) and IL-1 or metal inducible, human stromelysin and metallothionein (MT) gene promoters were tested for their activity in chondrocytes and synovial fibroblasts. Transient transfection with plasmi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Su S,Gedamu L,Dehnade F,Zafarullah M

    更新日期:1996-10-21 00:00:00

  • Sulphur islands in the Escherichia coli genome: markers of the cell's architecture?

    abstract::Two highly contrasted images depict genomes: at first sight, genes appear to be distributed randomly along the chromosome. In contrast, their organisation into operons (or pathogenicity islands) suggests that, at least locally, related functions are in physical proximity. Analysis of the codon usage bias in orthologou...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Rocha EP,Sekowska A,Danchin A

    更新日期:2000-06-30 00:00:00

  • The N-oligosaccharyltransferase complex from yeast.

    abstract::N-Oligosaccharyltransferase catalyzes the N-glycosylation of asparagine residues of nascent polypeptide chains in the endoplasmic reticulum, a pathway highly conserved in all eukaryotes. An enzymatically active complex was isolated from microsomal membranes from Saccharomyces cerevisiae, which is composed of four prot...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Knauer R,Lehle L

    更新日期:1994-05-09 00:00:00

  • Conformational change in thrombospondin induced by removal of bound Ca2+. A spin label approach.

    abstract::The effect of removal of Ca2+ bound to thrombospondin (TSP) on the protein structure in solution has been investigated using ESR spin-label techniques. A maleimide spin label was selectively attached to the free thiol group presumably near the carboxyl-terminal domain in which Ca2+-binding sites are situated. The ESR ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Slane JM,Mosher DF,Lai CS

    更新日期:1988-03-14 00:00:00

  • The primary structure of BSP-30K, a major lipid-, gelatin-, and heparin-binding glycoprotein of bovine seminal plasma.

    abstract::BSP-30K is a major acidic glycoprotein of bovine seminal plasma. It displays heparin-, gelatin-, and phospholipid-binding activities. BSP-30K binds to spermatozoa upon ejaculation and is thought to play a role in sperm capacitation. We have determined its amino acid sequence, disulfide bonds, and 0-glycosylation sites...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Calvete JJ,Mann K,Sanz L,Raida M,Töpfer-Petersen E

    更新日期:1996-12-09 00:00:00

  • The complete primary structure of GTP:AMP phosphotransferase from beef heart mitochondria.

    abstract::To complete the amino acid sequence of GTP:AMP phosphotransferase (MgGTP + AMP in equilibrium with MgGDP + ADP) from beef heart mitochondria it was necessary to sequence an intermediate region of about 33 residues after position 102 [(1984) Eur. J. Biochem. 143, 331-339] and find a suitable overlap with the rest of th...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tomasselli AG,Frank R,Schiltz E

    更新日期:1986-07-07 00:00:00