Abstract:
:In the present study, we have explored the impact of deleting a single allele of PDK1 in T cell progenitors on alpha/beta and gamma/delta T cell development. The data show that deleting a single allele of PDK1 allows differentiation of alpha/beta T cells but prevents their proliferative expansion in the thymus. Accordingly, mice with T cells that are haplo-insufficient for PDK1 have reduced numbers of thymocytes and alpha/beta peripheral T cells. T cell progenitors also give rise to gamma/delta T cells but in contrast to the loss of alpha/beta T cells in T-PDK1 null and haplo-insufficient mice, there were increased numbers of gamma/delta T cells. The production of alpha/beta T cells is dependent on the proliferative expansion of thymocytes and is determined by a balance between the frequency with which cells enter the proliferative phase of the cell cycle and rates of cell death. Herein, we show that PDK1 haplo-insufficient thymocytes have no defects in their ability to enter the cell cycle but show increased apoptosis. PDK1 thus plays a determining role in the development of alpha/beta T lymphocytes but does not limit gamma/delta T cell development.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kelly AP,Hinton HJ,Clarke RG,Cantrell DAdoi
10.1016/j.febslet.2006.03.022keywords:
subject
Has Abstractpub_date
2006-04-03 00:00:00pages
2135-40issue
8eissn
0014-5793issn
1873-3468pii
S0014-5793(06)00323-1journal_volume
580pub_type
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