Abstract:
:To identify suitable promoters for expressing exogenous genes in arthritic joints, the constitutive, simian virus 40 (SV 40) and IL-1 or metal inducible, human stromelysin and metallothionein (MT) gene promoters were tested for their activity in chondrocytes and synovial fibroblasts. Transient transfection with plasmids containing the reporter chloramphenicol acetyltransferase (CAT) gene attached to these promoters showed that SV40, stromelysin and MT promoters drove CAT expression with different strengths in primary bovine chondrocytes. The MTI-F and MT-IG gene promoters were also functional in human chondrocytes. The SV40, IL-1 inducible stromelysin-1 and MT-IG driven CAT activity was also detectable in human synoviocytes. Therefore, chondrocytes and synoviocytes have the trans-acting factors necessary for transcription from the respective promoters which may be conserved in bovine and human cells. These promoters could be useful for expressing potentially therapeutic anti-inflammatory and anti-erosive genes in arthritic joints.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Su S,Gedamu L,Dehnade F,Zafarullah Mdoi
10.1016/0014-5793(96)01037-xsubject
Has Abstractpub_date
1996-10-21 00:00:00pages
179-82issue
2-3eissn
0014-5793issn
1873-3468pii
0014-5793(96)01037-Xjournal_volume
395pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::In guinea pig caudate slices preloaded with [3H]choline, superfusion with medium containing Ca2+ and the phorbol ester TPA (12-O-tetradecanoyl phorbol 13-acetate) produced an increase in the Ca2+-dependent, depolarization-evoked release of acetylcholine (ACh), with no significant effect on the evoked Ca2+ influx. The ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80146-6
更新日期:1986-01-20 00:00:00
abstract::Rabbit red blood cells of various ages were separated on Percoll gradients and the activities of two large cytosolic proteases were measured. Both the multicatalytic protease (MCP), assayed by hydrolysis of fluorigenic peptides, and the 26 S ubiquitin/ATP-stimulated protease, assayed by degradation of ubiquitin-lysozy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80222-o
更新日期:1991-03-11 00:00:00
abstract::Glycosylated cellulases from Cellulomonas fimi were compared with their non-glycosylated counterparts synthesized in Escherichia coli from recombinant DNA. Glycosylation of the enzymes does not significantly affect their kinetic properties, or their stabilities towards heat and pH. However, the glycosylated enzymes ar...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81150-x
更新日期:1987-12-10 00:00:00
abstract::A peptide reproducing the G-H loop amino acid sequence of foot-and-mouth disease virus VP1 protein was fused to the solvent-exposed C-terminus of the bacteriophage P22 tailspike protein [Carbonell and Villaverde (1996) Gene, in press], a homotrimeric polypeptide with a strong beta-helical structure. This fusion does n...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01169-6
更新日期:1996-11-18 00:00:00
abstract::MicroRNAs (miRNA) play an important role in tumorigenesis, proliferation, and differentiation. Altered miRNA expression in cancer indicates that miRNAs can function as tumor suppressors or oncogenes. MiR-449c downregulation in non-small cell lung cancer (NSCLC) compared with normal lung tissues was investigated in thi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.03.006
更新日期:2013-05-02 00:00:00
abstract::The contributions of membrane type-1 matrix metalloproteinase (MT1-MMP) and of the glucose-6-phosphate transporter (G6PT) in sphingosine-1-phosphate (S1P)-mediated Ca(2+) mobilization were assessed in glioblastoma cells. We show that gene silencing of MT1-MMP or G6PT decreased the extent of S1P-induced Ca(2+) mobiliza...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.01.061
更新日期:2008-03-05 00:00:00
abstract::BSP-30K is a major acidic glycoprotein of bovine seminal plasma. It displays heparin-, gelatin-, and phospholipid-binding activities. BSP-30K binds to spermatozoa upon ejaculation and is thought to play a role in sperm capacitation. We have determined its amino acid sequence, disulfide bonds, and 0-glycosylation sites...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01310-5
更新日期:1996-12-09 00:00:00
abstract::We have studied the phosphorylation/dephosphorylation of several nuclear proteins in isolated nuclei from etiolated Avena seedlings as a function of red/far-red light. The effect of stimulatory (ADP-ribosylation by cholera toxin) or inhibitory (GDP beta S) conditions for GTP-binding proteins was also studied. Red or f...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80510-a
更新日期:1991-05-06 00:00:00
abstract::Physicochemical properties of proteins are controlled mainly by post-translational modifications such as amino acid phosphorylation. Although molecular dynamics simulations have been shown to be a valuable tool for studying the effects of phosphorylation on protein structure and dynamics, most of the previous studies ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13674
更新日期:2020-03-01 00:00:00
abstract::We have studied the interaction of the yeast RAS2 protein with guanine nucleotides using energetic parameters for the dissociation of RAS.nucleotide complexes. The results indicated that a Gly-->Ser substitution at position 82 led to an altered interaction with GppNHp and, to a lesser extent, also with GDP. It was als...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00521-4
更新日期:1994-06-27 00:00:00
abstract::Erythroid spectrin is the main component of the red cell membrane skeleton, which is very important in determining the shape, resistance to mechanical stresses and deformability of red cells. Previously we demonstrated that human erythroid alpha-spectrin is ubiquitinated in vitro and in vivo, and using recombinant pep...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02333-4
更新日期:2001-02-02 00:00:00
abstract::Chronic myelogenous leukemia (CML) is associated with the presence of the Philadelphia chromosome, which is generated by the reciprocal translocation of chromosomes 9 and 22. In the case of L6 (b2a2) mRNA, it is difficult to cleave the abnormal mRNA specifically because the mRNA includes no sequences that can be cleav...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01367-8
更新日期:1999-11-12 00:00:00
abstract::It has previously been shown that jasmonic acid affects the ethylene signaling pathway. EIN2 is a central component of ethylene signaling that is downstream of the receptors. EIN2 has previously been shown to be required for ethylene responses. We found that reducing jasmonic acid levels, either mutationally or chemic...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.11.030
更新日期:2013-01-16 00:00:00
abstract::The transdifferentiation of hepatic stellate cells into myofibroblast-like cells and the proliferation of the transdifferentiated cells are controlled by TGF-beta1. Little is known about the intracellular signal transducers of TGF-beta1. In this paper we show that in cultured hepatic stellate cells TGF-beta1 induces a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00024-0
更新日期:1997-02-10 00:00:00
abstract::3-(4'-Methyl-1'-naphthyl)-propionic acid, 1',4'-endoperoxide (NEPO) provides singlet state of oxygen (1O2, 1delta g) at 37 degrees C in sodium phosphate buffer (pH 7.2), acetate buffer (pH 4.5), methanol or chloroform, through the retro-Diels-Alder reaction. The total amount of 1O2 generated by NEPO was calculated usi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00822-9
更新日期:1998-07-31 00:00:00
abstract::The biochemical properties of the p51 subunit of HIV-1 reverse transcriptase (RT) were studied in order to understand its role in the heterodimeric form p66/p51 found in virions. A recombinant form of RT, p51/p51, expressed in yeast, was purified and characterized. The enzyme was affinity labeled using a 5' modified o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80202-r
更新日期:1992-04-13 00:00:00
abstract::Latent transforming growth factor-beta 1 binding protein (LTBP) is a constituent of the latent high molecular weight complex of TGF-beta 1 in human platelets. In the present communication, we show that LTBP binds Ca2+ in its free form as well as in the latent TGF-beta 1 complex. The binding of Ca2+ induces a structura...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80079-a
更新日期:1993-04-05 00:00:00
abstract::The nucleotide sequence of the dnaK operon cloned from Porphyromonas gingivalis revealed that the operon does not contain homologues of either dnaJ or grpE. However, there were two genes which encode small heat shock proteins immediately downstream from the dnaK and they were transcribed together with dnaK as one unit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00237-9
更新日期:1999-03-12 00:00:00
abstract::A human liver cDNA clone which encodes the UDP-glucose pyrophosphorylase was isolated by complementation of a bacterial galU mutant. The deduced amino acid sequence of the human enzyme comprised 508 amino acids with a calculated molecular mass of 56,950. The human enzyme significantly resembles those of potato tuber a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80213-e
更新日期:1993-08-23 00:00:00
abstract::We tested the effect of melatonin on membrane fluidity in microsomes of a rat liver model in which lipid peroxidation was induced by the addition of FeCl3, ADP and NADPH. Membrane fluidity was monitored using fluorescence spectroscopy and lipid peroxidation was estimated by quantifying malonaldehyde (MDA)+4-hydroxyalk...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00447-x
更新日期:1997-05-26 00:00:00
abstract::We have identified and analyzed a 27-nucleotide sequence (U5 repressive element, designated as U5RE) at the U5 region of the human T-cell leukemia virus type I (HTLV-I) long terminal repeat (LTR) which is required for HTLV-I basal transcriptional repression. The basal promoter strength of constructs that contained del...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01243-1
更新日期:1994-12-12 00:00:00
abstract::Oogenesis of amphibians is an atypical situation in which histone mRNA is polyadenylated. The poly(A) tract on H4 mRNA has been examined by S1 nuclease analysis. Throughout oogenesis the poly(A) tract is very short, and nonexistent on some mRNA molecules. The poly(A) tract is completely removed during maturation of th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81075-9
更新日期:1985-01-28 00:00:00
abstract::To identify genes whose expression is modulated by heregulin-beta1 (HRG), a regulatory polypeptide for mammary epithelial cells, we performed differential display screening of MCF-7 cell mRNA. One cDNA clone upregulated by HRG was identical to human ADP-ribosylation factor 3 (ARF3), a guanine nucleotide-binding protei...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02994-0
更新日期:2002-07-31 00:00:00
abstract::It is demonstrated by filter-binding assay that casein kinase 2 from Rana temporaria oocytes binds rRNA in vitro with high affinity. Ligand-blotting shows that rRNA-binding activity is inherent to alpha and alpha' subunits of the enzyme. Increase of pH from 6.5 to 7.5 has little effect on casein kinase but completely ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80593-r
更新日期:1991-06-03 00:00:00
abstract::Proteorhodopsin (PR), a retinal-containing seven transmembrane helix protein, functions as a light-driven proton pump. Using PCR, we isolated 18 PR variants originating from the surface of the Arctic Ocean. Their absorption maxima were between 517 and 546 nm at pH 7. One of the isolates turned out to be identical to G...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.04.025
更新日期:2008-05-28 00:00:00
abstract::Malignant mesothelioma (MM) is a fatal type of cancer. We studied the role of the redox-active protein thioredoxin-1 (Trx-1) in apoptosis induced in MM cells and their non-malignant counterparts (Met-5A) by alpha-tocopheryl succinate (alpha-TOS) and TNF-related apoptosis-inducing ligand (TRAIL). MM cells were suscepti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.04.019
更新日期:2006-05-15 00:00:00
abstract::In bacterial polypeptide synthesis aminoacyl-tRNA (aa-tRNA) bound to elongation factor Tu (EF-Tu) and GTP is part of a crucial intermediate ribonucleoprotein complex involved in the decoding of messenger RNA. The conformation and topology as well as the affinity of the macromolecules in this ternary aa-tRNA X EF-Tu X ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(87)80664-6
更新日期:1987-06-15 00:00:00
abstract::Prokaryotic aminoacylated-transfer RNAs often need to be efficiently segregated between translation and other cellular biosynthetic pathways. Many clinically relevant bacteria, including Streptococcus pneumoniae, Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa direct some aminoacylated-tRNA spe...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2013.07.036
更新日期:2013-09-17 00:00:00
abstract::The folding of HIV gp41 into a 6-helix bundle drives virus-cell membrane fusion. To examine the structural relationship between the 6-helix bundle core domain and other regions of gp41, we expressed in Escherichia coli, the entire ectodomain of HIV-2(ST) gp41 as a soluble, trimeric maltose-binding protein (MBP)/gp41 c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.04.054
更新日期:2004-06-04 00:00:00
abstract::Zinc metallopeptidases that contain the His-Glu-Xaa-Xaa-His (HEXXH) motif generally have a third ligand of the metal ion that may be either a Glu residue (in clan MA) or a His residue (in clan MB) (Rawlings and Barrett (1995) Methods Enzymol. 248, 183-228). Thimet oligopeptidase has not yet been assigned to either cla...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01032-1
更新日期:1998-09-11 00:00:00