Glycosylation of bacterial cellulases prevents proteolytic cleavage between functional domains.

abstract：:The interactions of DNA duplexes with 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), a potent mutacarcinogen isolated from tryptophan pyrolysate, have been studied using CD spectroscopy. The results are that (a) the spectral change of B-form DNA caused by the interaction with Trp-P-1 is biphasic, i.e. the enla...

journal_title：FEBS letters

authors： Inohara T,Tarui M,Doi M,Inoue M,Ishida T

更新日期：1993-06-21 00:00:00

abstract：:Alpha-toxins from scorpion venoms prolong the action potential of excitable cells by blocking sodium channel inactivation. We have purified bukatoxin, an alpha-toxin from scorpion (Buthus martensi Karsch) venom, to homogeneity. Bukatoxin produced marked relaxant responses in the carbachol-precontracted rat anococcygeu...

journal_title：FEBS letters

authors： Srinivasan KN,Nirthanan S,Sasaki T,Sato K,Cheng B,Gwee MC,Kini RM,Gopalakrishnakone P

更新日期：2001-04-13 00:00:00

abstract：:In the present work, a new endopin-like serpin designed mEndopin 1B was purified from bovine muscle. Biochemical characterizations (amino acid sequencing and Maldi-Tof mass spectrometry peptide mapping) demonstrated that the purified protein is different from the previously described Endopin 1, renamed mEndopin 1A. Th...

journal_title：FEBS letters

authors： Herrera-Mendez CH,Brémaud L,Coulis G,Pélissier P,Sentandreu MA,Aubry L,Delourme D,Chambon C,Maftah A,Leveziel H,Ouali A

更新日期：2006-06-12 00:00:00

abstract：:Human aminopeptidase N carries an apical sorting signal on its ectodomain necessary for its correct transport to the apical membrane in Madin-Darby canine kidney cells. To determine whether the apical sorting signal is localized in the serine/threonine rich stalk or in the catalytic head group, anchor/stalk-minus amin...

journal_title：FEBS letters

authors： Vogel LK,Norén O,Sjöström H

更新日期：1992-08-10 00:00:00

abstract：:Pleckstrin homology (PH) domains are protein modules found in proteins involved in many cellular processes. The majority of PH domain-containing proteins require membrane association for their function. It has been shown that most PH domains interact directly with the cell membrane by binding to phosphoinositides with...

journal_title：FEBS letters

authors： Maffucci T,Falasca M

更新日期：2001-10-12 00:00:00

abstract：:A Ca2+ indicator arsenazo III was used to demonstrate calcium uptake activity of symbiosomes and the peribacteroid membrane (PBM) vesicles isolated from broad bean root nodules and placed in the medium containing ATP and Mg2+ ions. This process was shown to be rapidly stopped by vanadate, completely reversed in the pr...

journal_title：FEBS letters

authors： Andreev IM,Dubrovo PN,Krylova VV,Izmailov SF

更新日期：1999-03-19 00:00:00

abstract：:Using radiolabeled specific cDNA glutamine synthetase mRNA could be detected by in situ hybridization exclusively within those few perivenous hepatocytes which stained immunocytochemically for glutamine synthetase. This localization of glutamine synthetase mRNA was recently reported by Moorman et al. [(1988) J. Histoc...

journal_title：FEBS letters

authors： Gebhardt R,Ebert A,Bauer G

更新日期：1988-12-05 00:00:00

abstract：:PDZ domains are thought to act as protein-binding modules mediating the clustering of membrane and membrane-associated proteins. The INAD protein has been shown to interact via a PDZ domain with the calcium channel TRP which contributes to capacitative calcium entry into Drosophila photoreceptor cells. We have cloned ...

journal_title：FEBS letters

authors： Philipp S,Flockerzi V

更新日期：1997-08-18 00:00:00

abstract：:The distribution of phosphatidylcholine (PC) and sphingomyelin (SM) between the solubilized (micellar) and non-solubilized (lamellar) fractions arising from bilayers composed of PC and SM, with or without cholesterol (Chol) has been measured under conditions of partial, incomplete solubilization by Triton X-100. Quant...

journal_title：FEBS letters

authors： Ahyayauch H,Collado MI,Goñi FM,Lichtenberg D

更新日期：2009-09-03 00:00:00

abstract：:The cytosolic NADP(+)-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase is ubiquitously expressed in all mouse tissues and cell lines examined. Northern analyses of the RNA indicated that there is an extensive variation in the levels of mRNA in ...

journal_title：FEBS letters

authors： Peri KG,MacKenzie RE

更新日期：1991-12-02 00:00:00

abstract：:The accumulation of amyloid-beta (Aβ) and tau aggregates is a pathological hallmark of Alzheimer's disease. Both polypeptides form fibrillar deposits, but several lines of evidence indicate that Aβ and tau form toxic oligomeric aggregation intermediates. Depleting such structures could thus be a powerful therapeutic s...

journal_title：FEBS letters

authors： Wobst HJ,Sharma A,Diamond MI,Wanker EE,Bieschke J

更新日期：2015-01-02 00:00:00

abstract：:Ferric reductase B (FerB) is a flavin mononucleotide (FMN)-containing NAD(P)H:acceptor oxidoreductase structurally close to the Gluconacetobacter hansenii chromate reductase (ChrR). The crystal structure of ChrR was previously determined with a chloride bound proximal to FMN in the vicinity of Arg101, and the authors ...

journal_title：FEBS letters

authors： Sedláček V,Kučera I

更新日期：2019-04-01 00:00:00

abstract：:The 39-kDa fragment of oat phytochrome phyA, obtained by tryptic digestion at the amino acids 65 and 425, was studied by resonance Raman spectroscopy. The parent state P(r) reveals far-reaching similarities with that of the native phytochrome implying that the structures of the tetrapyrrole chromophore and its immedia...

journal_title：FEBS letters

authors： Kneip C,Schlamann W,Braslavsky SE,Hildebrandt P,Schaffner K

更新日期：2000-10-06 00:00:00

abstract：:Targeting of preproteins to mitochondria and their translocation across the outer membrane are mediated by the mitochondrial receptor complex. This protein complex contains the import receptors MOM19 and MOM72 and the general insertion pore GIP. All seven components of the receptor complex are synthesized in the cytos...

journal_title：FEBS letters

authors： Keil P,Pfanner N

更新日期：1993-04-26 00:00:00

abstract：:The crystal structure of the free form of IF1 from Mycobacterium tuberculosis has been determined at 1.47 A resolution. The structure adopts the expected OB fold and matches the high structural conservation among IF1 orthologues. In order to further explore the function of Mtb-IF1, we built a model of its interaction ...

journal_title：FEBS letters

authors： Hatzopoulos GN,Mueller-Dieckmann J

更新日期：2010-03-05 00:00:00

abstract：:Several neurodegenerative disorders are characterized by the accumulation of proteinaceous inclusions in the central nervous system. These inclusions are frequently composed of a mixture of aggregation-prone proteins. Here, we used a bimolecular fluorescence complementation assay to study the initial steps of the co-a...

journal_title：FEBS letters

authors： Herrera F,Outeiro TF

更新日期：2012-01-02 00:00:00

abstract：:Glycine amide (GlyAd), a typically amidated amino acid, is a versatile additive that suppresses protein aggregation during refolding, heat treatment, and crystallization. In spite of its effectiveness, the exact mechanism by which GlyAd suppresses protein aggregation remains to be elucidated. Here, we show the crystal...

journal_title：FEBS letters

authors： Ito L,Shiraki K,Makino M,Hasegawa K,Kumasaka T

更新日期：2011-02-04 00:00:00

abstract：:γ-Glutamyl kinase (GK; the PRO1 gene product) is a key enzyme in the Saccharomyces cerevisiae proline biosynthesis pathway. Δpro1 cells are more sensitive to various stresses than wild-type cells, suggesting that GK has an alternative function independent of proline biosynthesis. We show that PRO1 genetically interact...

journal_title：FEBS letters

authors： Tatehashi Y,Watanabe D,Takagi H

更新日期：2016-09-01 00:00:00

abstract：:Protein kinases play a crucial role in the regulation of cellular processes. Most eukaryotes reserve about 2.5% of their genes for protein kinases. We analysed the genome of the single-celled ciliate Paramecium tetraurelia and identified 2606 kinases, about 6.6% of its genes, representing the largest kinome to date. A...

journal_title：FEBS letters

authors： Bemm F,Schwarz R,Förster F,Schultz J

更新日期：2009-11-19 00:00:00

abstract：:The Escherichia coli signal transduction protein PII, product of the glnB gene, was overproduced and purified. The predicted molecular weight of the protein based on the correct nucleotide sequence is 12,427 and is very close to the value 12,435 obtained by matrix-assisted laser desorption mass spectrometry. Hexagonal...

journal_title：FEBS letters

authors： Vasudevan SG,Gedye C,Dixon NE,Cheah E,Carr PD,Suffolk PM,Jeffrey PD,Ollis DL

更新日期：1994-01-17 00:00:00

abstract：:Cyclooxygenases (COXs) are key enzymes in the conversion of arachidonic acid into prostanoids which are involved in apoptosis and inflammation. Two distinct COXs have been identified: COX-1 which is constitutively expressed and COX-2 which is induced by different products such as tumor promoters or growth factors. Pre...

journal_title：FEBS letters

authors： Moalic S,Liagre B,Corbière C,Bianchi A,Dauça M,Bordji K,Beneytout JL

更新日期：2001-10-12 00:00:00

abstract：:Nuclear cap-binding protein (CBP) 80/20-dependent translation (CT) is one of the targets for miRNA-mediated gene silencing. Here, we provide evidence that human argonaute 2 (Ago2) competes with CBP80/20 for cap-association, inhibiting CT and thus nonsense-mediated mRNA decay (NMD), which is tightly coupled to CT. Teth...

journal_title：FEBS letters

authors： Choe J,Cho H,Chi SG,Kim YK

更新日期：2011-09-02 00:00:00

abstract：:The cDNAs of several snake venom zinc endopeptidases code for a putative propeptide, which includes the conserved cysteine-containing sequence PKMCGVT. It has been suggested that binding of the cysteine thiol function to the active-site zinc, resulting in inactivation of the catalytic domain, occurs in a mode similar ...

journal_title：FEBS letters

authors： Grams F,Huber R,Kress LF,Moroder L,Bode W

更新日期：1993-11-29 00:00:00

abstract：:Liver microsomes contain a vitamin K and O2-dependent carboxylase that converts peptide-bound glutamyl residues to gamma-carboxyglutamate residues. The peptide Boc-O-phospho-Ser-O-phospho-Ser-Leu-OMe has now been synthesized. This peptide inhibits the carboxylation of endogenous protein precursors by a detergent-solub...

journal_title：FEBS letters

authors： Rich DH,Kawai M,Goodman HL,Engelke J,Suttie JW

更新日期：1983-02-07 00:00:00

abstract：:The FMN-heme interdomain (intraprotein) electron transfer (IET) kinetics in full length and oxygenase/FMN (oxyFMN) construct of human iNOS were determined by laser flash photolysis over the temperature range from 283 to 304K. An appreciable increase in the rate constant value was observed with an increase in the tempe...

journal_title：FEBS letters

authors： Li W,Chen L,Fan W,Feng C

更新日期：2012-01-20 00:00:00

abstract：:In Alzheimer disease (AD) the microtubule associated protein (MAP) tau is hyperphosphorylated at several sites. In the present study, like AD tau, tau in the human neuroblastoma SH-SY5Y was found to be hyperphosphorylated, at Ser-199/202, Thr-231, Ser-396 and Ser-404. However, in contrast to AD, the tau in SY5Y cells ...

journal_title：FEBS letters

authors： Tanaka T,Iqbal K,Trenkner E,Liu DJ,Grundke-Iqbal I

更新日期：1995-02-20 00:00:00

abstract：:DNA complementary to the rat hypothalamic mRNA coding for the corticotropin-releasing factor precursor (prepro-CRF) has been cloned by screening a cDNA library with a human genomic DNA probe. Nucleotide sequence analysis of the cloned cDNA has revealed that rat prepro-CRF consists of 187 amino acid residues including ...

journal_title：FEBS letters

authors： Jingami H,Mizuno N,Takahashi H,Shibahara S,Furutani Y,Imura H,Numa S

更新日期：1985-10-21 00:00:00

abstract：:Mitochondrial dihydroorotate dehydrogenase (DHOdehase; EC 1.3.99.11) is a target of anti-proliferative, immunosuppressive and anti-parasitic agents. Here, redoxal, (2,2'-[3,3'-dimethoxy[1, 1'-biphenyl]-4,4'-diyl)diimino]bis-benzoic acid, was studied with isolated mitochondria and the purified recombinant human and rat...

journal_title：FEBS letters

authors： Knecht W,Löffler M

更新日期：2000-02-04 00:00:00

abstract：:To better understand the mechanism of intracellular Ca2+ mobilization, mouse oocytes were micro-injected with 'caged'-inositol-1,4,5 triphosphate caged-InsP3) together with the Ca2+ indicator Fluo-3 to directly induce and monitor Ca2+ redistribution. Photo-released InsP3 elicits [Ca2+]i changes exhibiting several kine...

journal_title：FEBS letters

authors： Peres A

更新日期：1990-11-26 00:00:00

abstract：:To date the only tRNAs containing nucleosides modified with a selenium (5-carboxymethylaminomethyl-2-selenouridine and 5-methylaminomethyl-2-selenouridine) have been found in bacteria. By using tRNA anticodon-stem-loop fragments containing S2U, Se2U, or geS2U, we found that in vitro tRNA 2-selenouridine synthase (SelU...

journal_title：FEBS letters

authors： Sierant M,Leszczynska G,Sadowska K,Komar P,Radzikowska-Cieciura E,Sochacka E,Nawrot B

更新日期：2018-07-01 00:00:00