Abstract:
:To better understand the mechanism of intracellular Ca2+ mobilization, mouse oocytes were micro-injected with 'caged'-inositol-1,4,5 triphosphate caged-InsP3) together with the Ca2+ indicator Fluo-3 to directly induce and monitor Ca2+ redistribution. Photo-released InsP3 elicits [Ca2+]i changes exhibiting several kinetic phases and threshold behaviour. Often Ca2+ oscillations were induced after a single InsP3 pulse. Autoregenerative Ca2+ transients could also be induced by injections of Ca2+ itself, demonstrating unequivocally the presence of a Ca2(+)-induced Ca2(+)-release mechanism in these cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Peres Adoi
10.1016/0014-5793(90)81474-3subject
Has Abstractpub_date
1990-11-26 00:00:00pages
213-6issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(90)81474-3journal_volume
275pub_type
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