Abstract:
:Perturbation of the aerobic steady-state in a chemostat culture of the ethanol-producing bacterium Zymomonas mobilis with a small pulse of ethanol causes a burst of ethanol oxidation, although the reactant ratio of the alcohol dehydrogenase (ADH) reaction ([NADH][acetaldehyde][H(+)])/([ethanol][NAD(+)]) remains above the K(eq) value. Simultaneous catalysis of ethanol synthesis and oxidation by the two ADH isoenzymes, residing in different redox microenvironments, has been proposed previously. In the present study, this hypothesis is verified by construction of an ADH-deficient strain and by demonstration that it lacks the oxidative burst in response to perturbation of its aerobic steady-state with ethanol.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kalnenieks U,Galinina N,Toma MM,Pickford JL,Rutkis R,Poole RKdoi
10.1016/j.febslet.2006.08.034subject
Has Abstractpub_date
2006-09-18 00:00:00pages
5084-8issue
21eissn
0014-5793issn
1873-3468pii
S0014-5793(06)01006-4journal_volume
580pub_type
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