Abstract:
:Here, we used a reductant, N-acetyl-L-cysteine (NAC), to investigate the redox-sensitive step(s) in the signalling pathway from the tumor necrosis factor (TNF) receptor to nuclear factor kappaB (NF-kappaB). We found that NAC suppressed NF-kappaB activation triggered by TNF or by overexpression of either the TNF receptor-associated death domain protein, TNF receptor-associated factor 2, NF-kappaB-inducing kinase (NIK), or IkappaB kinases (IKKalpha and IKKbeta). NAC also suppressed the TNF-induced activation of IKKalpha and IKKbeta, phosphorylation and degradation of IkappaB, and nuclear translocation of NF-kappaB. Furthermore, NAC suppressed the activation of IKKalpha and IKKbeta triggered by the overexpression of NIK. These results indicate that IKKalpha and IKKbeta are subject to redox regulation in the cells, and that NAC inhibits NF-kappaB activation through the suppression of these kinases.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Oka S,Kamata H,Kamata K,Yagisawa H,Hirata Hdoi
10.1016/s0014-5793(00)01464-2keywords:
subject
Has Abstractpub_date
2000-04-28 00:00:00pages
196-202issue
2-3eissn
0014-5793issn
1873-3468pii
S0014579300014642journal_volume
472pub_type
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