Molecular cloning of a novel C or gamma type chemokine, SCM-1.

abstract：:Differences in the metabolism of cancer cells or cancer stem cells (CSCs) as compared to normal cells have provided avenues to safely target cancers. To discover metabolic inhibitors of CSCs, we performed alkaline phosphatase- and tumoursphere-based drug screening using induced cancer stem cell-like cells. From the sc...

journal_title：FEBS letters

authors： Subedi A,Muroi M,Futamura Y,Kawamura T,Aono H,Nishi M,Ryo A,Watanabe N,Osada H

更新日期：2019-04-01 00:00:00

abstract：:A cell culture model of blood-brain barrier (BBB, coculture of rat brain endothelial cells with rat astrocytes) was used to investigate the effect of nitric oxide (.NO) on the damage of the BBB induced by hypoxia/reoxygenation (H/R). Permeability coefficient of fluorescein across the endothelium was used as a marker o...

journal_title：FEBS letters

authors： Utepbergenov DI,Mertsch K,Sporbert A,Tenz K,Paul M,Haseloff RF,Blasig IE

更新日期：1998-03-13 00:00:00

abstract：:We have used ultraviolet laser crosslinking to characterize the DNA-binding properties of highly purified yeast topoisomerase II in the absence of ATP. A single 5 ns, 20 mJ pulse of 266 nm light produced optimal crosslinking to a short DNA duplex, with an efficiency of 0.25%. An equilibrium binding constant (Keq) of 1...

journal_title：FEBS letters

authors： Hung F,Luo D,Sauvé DM,Muller MT,Roberge M

更新日期：1996-02-12 00:00:00

abstract：:Ku, a heterodimer of 70- and 80-kDa subunits, plays a general role in the metabolism of DNA ends in eukaryotic cells, including double-strand DNA break repair, V(D)J recombination, and maintenance of telomeres. We have utilized the yeast two-hybrid system to identify Ku70-interacting proteins other than Ku80. Two reac...

journal_title：FEBS letters

authors： Song K,Jung D,Jung Y,Lee SG,Lee I

更新日期：2000-09-08 00:00:00

abstract：:Endothelial monocyte-activating polypeptide II (EMAP II) is a proinflammatory cytokine and a chemoattractant for leukocytes. The mature cytokine is formed in apoptotic cells by cleavage of the precursor proEMAP II. Here we show that caspase-7 is capable of cleaving proEMAP II in vitro. A proEMAP II mutant, in which th...

journal_title：FEBS letters

authors： Behrensdorf HA,van de Craen M,Knies UE,Vandenabeele P,Clauss M

更新日期：2000-01-21 00:00:00

abstract：:Winter flounder contains two distinct anti-freeze protein isoforms, which are the liver-type extracellular anti-freeze proteins and the skin-type intracellular anti-freeze protein. The skin-type anti-freeze proteins exhibit lower anti-freeze activities than the liver-type isoforms and this might be due to their lackin...

journal_title：FEBS letters

authors： Lin Q,Ewart KV,Yang DS,Hew CL

更新日期：1999-06-25 00:00:00

abstract：:Insulin causes a translocation of glucose transporters from intracellular microsomes to the plasma membrane in adipocytes. To determine whether insulin has a similar effect in rat hindlimb muscles, we used glucose-inhibitable cytochalasin B binding to estimate the number of glucose transporters in membrane fractions f...

journal_title：FEBS letters

authors： Klip A,Ramlal T,Young DA,Holloszy JO

更新日期：1987-11-16 00:00:00

abstract：:Plasminogen activation is regulated by the interaction between urokinase-type plasminogen activator (uPA) and its specific glycolipid-anchored cell surface receptor (uPAR). uPAR is composed of three homologous domains and is the only multi-domain member of the Ly-6 family of glycolipid-anchored membrane proteins. Rece...

journal_title：FEBS letters

authors： Ploug M,Ellis V

更新日期：1994-08-01 00:00:00

abstract：:The hepatitis B viral X protein (HBx) is a multifunctional protein that plays a role in the activation of the NF-kappaB signaling pathway. In this study, we demonstrate that HBx up-regulates expression of TANK-binding kinase-1 (TBK1) at the transcriptional level. Increased levels of TBK1 protein then induce the phosph...

journal_title：FEBS letters

authors： Kim HR,Lee SH,Jung G

更新日期：2010-02-05 00:00:00

abstract：:We focused on the functional involvement of transforming growth factor-beta-activated kinase 1 (TAK1) in transcriptional regulation of interleukin-2 (IL-2) in T cells. Costimulation of Jurkat cells with 12-O-tetradecanoylphorbol-13-acetate and A23187 leads to a rapid phosphorylation of TAK1 and TAK1-binding protein 1 ...

journal_title：FEBS letters

authors： Sakurai H,Singhirunnusorn P,Shimotabira E,Chino A,Suzuki S,Koizumi K,Saiki I

更新日期：2005-12-05 00:00:00

abstract：:Currently described substrates of the bacterial Tat protein transport system are directed for export by signal peptides containing a pair of invariant arginine residues. The signal peptide of the TtrB subunit of Salmonella enterica tetrathionate reductase contains a single arginine residue but is nevertheless able to ...

journal_title：FEBS letters

authors： Hinsley AP,Stanley NR,Palmer T,Berks BC

更新日期：2001-05-18 00:00:00

abstract：:Peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1alpha), a highly inducible transcriptional coactivator regulating energy homeostasis, is down-regulated in hepatoma tissues. To dissect its role in hepato-tumorigenesis, Ingenuity Pathway Analysis was applied to construct pathways affected by P...

journal_title：FEBS letters

authors： Lee HJ,Su Y,Lui WY,Chau GY,Yin PH,Lee HC,Chi CW

更新日期：2008-03-05 00:00:00

abstract：:Large amounts of refined sequence material in the form of predicted, curated and annotated genes and expressed sequences tags (ESTs) have recently been added to the NCBI databases. We matched the transcript-sequences of RefSeq, Ensembl and dbEST in an attempt to provide an updated overview of how many unique human gen...

journal_title：FEBS letters

authors： Larsson TP,Murray CG,Hill T,Fredriksson R,Schiöth HB

更新日期：2005-01-31 00:00:00

abstract：:The extents to which different rate constants limit the steady-state rate of an isolated enzyme can be quantified as the control coefficients of those constants and elemental steps. We have found that the sum of the control coefficients of rate constants characterising unidirectional rates depleting a particular enzym...

journal_title：FEBS letters

authors： Kholodenko BN,Westerhoff HV,Brown GC

更新日期：1994-07-25 00:00:00

abstract：:Extracellular laccase (benzenediol: oxygen oxidoreductase EC 1.10.3.2) from the lignin-degrading fungus, Phlebia radiata, was shown to contain a novel combination of electron carriers as its prosthetic groups. In addition to two copper atoms per enzyme molecule, one molecule of PQQ was included as a cofactor. The EPR ...

journal_title：FEBS letters

authors： Karhunen E,Niku-Paavola ML,Viikari L,Haltia T,van der Meer RA,Duine JA

更新日期：1990-07-02 00:00:00

abstract：:Gene conservation, duplication and constitutive expression are intricately linked and strong predictors of essentiality. Here, we introduce metrics based on diversity indices to measure gene conservation, duplication and constitutive expression and validate them by measuring their performance in prediction of essentia...

journal_title：FEBS letters

authors： Arun PV,Miryala SK,Chattopadhyay S,Thiyyagura K,Bawa P,Bhattacharjee M,Yellaboina S

更新日期：2016-05-01 00:00:00

abstract：:Benign familial neonatal convulsions, an autosomal dominant epilepsy of newborns, are linked to mutations affecting two six-transmembrane potassium channels, KCNQ2 and KCNQ3. We isolated four splice variants of KCNQ2 in human brain. Two forms generate, after transient expression in COS cells, a potassium-selective cur...

journal_title：FEBS letters

authors： Tinel N,Lauritzen I,Chouabe C,Lazdunski M,Borsotto M

更新日期：1998-11-06 00:00:00

abstract：:Meprin α and β, members of the astacin family of zinc metalloproteinases, are unique plasma membrane and secreted proteases known to cleave a wide range of biological substrates involved in inflammation, cancer and fibrosis. In this study, we identified proMMP-9 as a novel substrate and show that aminoterminal meprin-...

journal_title：FEBS letters

authors： Geurts N,Becker-Pauly C,Martens E,Proost P,Van den Steen PE,Stöcker W,Opdenakker G

更新日期：2012-12-14 00:00:00

abstract：:By sequence analysis we show that the U104 domain found in the UNC104 subfamily of kinesins is a forkhead homology-associated domain (FHA). A combination of limited proteolysis, mass spectroscopy, and physicochemical analysis define this domain as a genuine autonomously folding domain. Our data show that the FHA domai...

journal_title：FEBS letters

authors： Westerholm-Parvinen A,Vernos I,Serrano L

更新日期：2000-12-15 00:00:00

abstract：:A novel gene encoding a new H3 histone varian (H3L) has been identified in P. lividus sea urchin embryo. It encodes a H3 histone protein showing the S.//.A.IG amino acid motif typical of the replication independent H3.3 variants but in a mRNA showing the 3' terminal stem-loop nucleotide sequence that is typical of the...

journal_title：FEBS letters

authors： Fucci L,Forte A,Mancini P,Affaitati A,Branno M,Aniello F,Geraci G

更新日期：1997-04-21 00:00:00

abstract：:The structural characteristics of the three nuclear phosphoproteins of the high mobility group A family are outlined and related to their participation in chromatin structure alteration in many biological processes such as gene expression, neoplastic transformation, differentiation, and apoptosis. The elevated express...

journal_title：FEBS letters

authors： Sgarra R,Rustighi A,Tessari MA,Di Bernardo J,Altamura S,Fusco A,Manfioletti G,Giancotti V

更新日期：2004-09-10 00:00:00

abstract：:Parkinson's disease is the second most common neurodegenerative disease, and results from loss of dopaminergic neurons in the substantia nigra. The aggregation and fibrillation of alpha-synuclein in the form of intracellular proteinaceous aggregates (Lewy bodies and Lewy neurites) have been implicated as a causative f...

journal_title：FEBS letters

authors： Uversky VN,Fink AL

更新日期：2002-07-03 00:00:00

abstract：:We report phosphorylated and ubiquitinated aggregates of TAR DNA binding protein of 43 kDa (TDP-43) in SH-SY5Y cells similar to those in brains of amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration with ubiquitinated inclusions (FTLD-U). Two candidate sequences for the nuclear localization signa...

journal_title：FEBS letters

authors： Nonaka T,Arai T,Buratti E,Baralle FE,Akiyama H,Hasegawa M

更新日期：2009-01-22 00:00:00

abstract：:Novel catechin derivatives obtained from grape procyanidins and l-cysteine scavenge free radicals by hydrogen atom donation, rather than electron transfer, and reduce cell viability in A375 and M21 melanoma cells. In particular, 4beta-(S-cysteinyl)epicatechin 3-O-gallate has a free radical scavenging capacity as stron...

journal_title：FEBS letters

authors： Lozano C,Torres JL,Julià L,Jimenez A,Centelles JJ,Cascante M

更新日期：2005-08-15 00:00:00

abstract：:Chloride intracellular channel proteins (CLICs) are distinct from most ion channels in that they have both soluble and integral membrane forms. CLICs are highly conserved in chordates, with six vertebrate paralogues. CLIC-like proteins are found in other metazoans. CLICs form channels in artificial bilayers in a proce...

journal_title：FEBS letters

authors： Littler DR,Harrop SJ,Goodchild SC,Phang JM,Mynott AV,Jiang L,Valenzuela SM,Mazzanti M,Brown LJ,Breit SN,Curmi PM

更新日期：2010-05-17 00:00:00

abstract：:The integrin alpha IIb beta 3-mediated redistribution of the tyrosine kinases pp125FAK and pp60Src and the small GTP-binding proteins CDC42Hs and Rap1B from the membrane skeleton to the cytoskeleton was found to be reversible: upon prolonged platelet aggregation (up to 15 min) induced by the thrombin-receptor activati...

journal_title：FEBS letters

authors： Dash D,Aepfelbacher M,Siess W

更新日期：1995-04-24 00:00:00

abstract：:Oxygen is an essential element in the survival of complex organisms, however the level of oxygen, low or high, can be a source of stress depending on the biological context. Low levels of oxygen in tissues (hypoxia) can be the consequence of a number of pathophysiological conditions including ischemic disorders and ca...

journal_title：FEBS letters

authors： Brahimi-Horn MC,Pouysségur J

更新日期：2007-07-31 00:00:00

abstract：:Mutation of Thr90 to Ala has a profound effect on bacteriorhodopsin properties. T90A shows about 20% of the proton pumping efficiency of wild type, once reconstituted into liposomes. Mutation of Thr90 influences greatly the Schiff base/Asp85 environment, as demonstrated by altered lambda(max) of 555 nm and pK(a) of As...

journal_title：FEBS letters

authors： Perálvarez A,Barnadas R,Sabés M,Querol E,Padrós E

更新日期：2001-11-23 00:00:00

abstract：:It is demonstrated by filter-binding assay that casein kinase 2 from Rana temporaria oocytes binds rRNA in vitro with high affinity. Ligand-blotting shows that rRNA-binding activity is inherent to alpha and alpha' subunits of the enzyme. Increase of pH from 6.5 to 7.5 has little effect on casein kinase but completely ...

journal_title：FEBS letters

authors： Kandror KV,Kapkov DV,Turapov OA,Stepanov AS

更新日期：1991-06-03 00:00:00

abstract：:The effect of all possible codons corresponding to the second and third amino acid (isoleucine and threonine) on the expression level of hirudin in E. coli has been analysed. These levels could not be correlated with changes in primary and secondary mRNA structure. A decrease in the rate of synthesis and of product ac...

journal_title：FEBS letters

authors： Degryse E

更新日期：1990-08-20 00:00:00