Abstract:
:We have expressed the cloned rat kappa-opioid receptor in human embryonic kidney 293 cells and studied the ability of kappa-selective ligands to inhibit adenylyl cyclase. In transfected 293 cells, activation of the kappa-opioid receptor by U50,488 and the dynorphins resulted in the inhibition of cAMP accumulation. The inhibitory response was sensitive to pertussis toxin and highly selective for kappa-agonists; neither mu- nor delta-opioids were able to activate the kappa-opioid receptor. Upon co-transfection with the alpha subunit of Gz, inhibition of cAMP accumulation by kappa-agonist became refractory to pertussis toxin, indicating that the kappa-opioid receptor can couple to both G(i) and Gz proteins.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Lai HW,Minami M,Satoh M,Wong YHdoi
10.1016/0014-5793(95)00088-qsubject
Has Abstractpub_date
1995-02-20 00:00:00pages
97-9issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(95)00088-Qjournal_volume
360pub_type
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