Abstract:
:The cDNA for the nearly full-length rabbit muscle glycogen phosphorylase mRNA has been isolated and sequenced. The cDNA is rich in G and C nucleotides. This feature is especially striking at the 3rd position of codons, where 86% of the 843 amino acid codons terminate with G or C. Methionine, presumably the initiation residue, is found at position-1, suggesting that the removal of only a single methionine residue precedes the amino-terminal acetylation at serine. Eight differences between the deduced amino acid sequence and the previously determined protein sequence are discussed.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Nakano K,Hwang PK,Fletterick RJdoi
10.1016/0014-5793(86)80829-8subject
Has Abstractpub_date
1986-08-18 00:00:00pages
283-7issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(86)80829-8journal_volume
204pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The binding of protein HU from Escherichia coli to nucleic acids was investigated by affinity chromatography under various conditions, by a nitrocellulose retention assay and by isopycnic centrifugations in metrizamide gradients. The results indicate that HU has a preference for binding to RNA and single-stranded DNA ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80753-5
更新日期:1985-06-03 00:00:00
abstract::Understanding specific cargo distribution in differentiated cells is a major challenge. Trafficking kinesin proteins (TRAKs) are kinesin adaptors. They bind the cargo binding domain of kinesin-1 motor proteins forming a link between the motor and their cargoes. To refine the TRAK1/2 binding sites within the kinesin-1 ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.09.049
更新日期:2013-11-29 00:00:00
abstract::Antibodies to a synthetic peptide from the 'amphipathic helix' of the alpha-chain of the nicotinic acetylcholine receptor (nAChR) bound both to detergent-solubilised and membrane-bound nAChR, indicating that this region, suggested as a component of the transmembrane ion channel in one model, is not buried in the membr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80374-5
更新日期:1987-08-31 00:00:00
abstract::Synaptotagmin I, the calcium sensor for neurotransmission, is palmitoylated. We have identified the palmitoylation sites as five cysteine residues located between the transmembrane and cytoplasmic regions. In contrast to wild-type synaptotagmin, the non-acylated mutant is not converted to the endoglycosidase-H-resista...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00449-6
更新日期:2003-06-05 00:00:00
abstract::A cytochrome c (cyt. c) was solubilized with Triton-X-100 and co-purified with cytochrome c oxidase from membranes of chemotrophically grown cells of Rhodopseudomonas capsulata. Cyt. c and cytochrome oxidase were separated on Sephadex G-50 columns. Antibodies against cytochrome c2 from the same bacterium did not cross...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80390-1
更新日期:1983-02-21 00:00:00
abstract::The accumulation of pre-PhoS under conditions of PhoS overproduction has been previously described. It is now demonstrated that during the induction of PhoS, a delay in the completion of polypeptide chain elongation can be detected. This delay is related to the extent of jamming of export sites by pre-PhoS or by other...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80204-6
更新日期:1986-02-03 00:00:00
abstract::The cDNAs of several snake venom zinc endopeptidases code for a putative propeptide, which includes the conserved cysteine-containing sequence PKMCGVT. It has been suggested that binding of the cysteine thiol function to the active-site zinc, resulting in inactivation of the catalytic domain, occurs in a mode similar ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80443-x
更新日期:1993-11-29 00:00:00
abstract::Several protein kinase C (PKC) isozymes were analyzed by immunoblot and immunocytochemistry in cultures of hippocampal neurones at several stages of differentiation. Our findings reveal the existence of two distinct patterns of expression. Firstly, conventional PKC isozymes alpha, beta and gamma, that are expressed at...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00303-q
更新日期:1995-04-24 00:00:00
abstract::The secretion mechanisms of cathepsin L from osteoclasts in the process of bone resorption were investigated. The increases in bone pit numbers formed take place by PTH addition in parallel with the increases of cathepsin L and/or L-like proteinase activities in the culture medium of bone cells, and these were suppres...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80522-9
更新日期:1994-04-11 00:00:00
abstract::Mitochondria consume nitric oxide (NO) mainly through reaction with superoxide anion (O(2)(-)). Here, we analyzed the O(2)(-) sources for NO degradation by isolated rat liver mitochondria. Electron leakage from complex III and reverse electron transport to complex I accounted for O(2)(-)-dependent NO degradation by mi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.06.012
更新日期:2009-07-07 00:00:00
abstract::The plant-specific GAI-RGA-and-SCR (GRAS) family of proteins function as transcriptional regulators and play critical roles in development and signalling. Recent structural studies have shed light on the molecular functions at the structural level. The conserved GRAS domain comprises an α-helical cap and α/β core subd...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12987
更新日期:2018-02-01 00:00:00
abstract::In the central nervous system, synaptic signal transduction depends on the regulation of neurotransmitter receptors by interacting proteins. Here, we searched for proteins interacting with two metabotropic glutamate receptor type 8 isoforms (mGlu8a and mGlu8b) and identified RanBPM. RanBPM is expressed in several brai...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.06.010
更新日期:2008-07-09 00:00:00
abstract::Three major UDP-glucuronyltransferase isoenzymes (50-54 kDa) have been identified by immunoblot analysis. Bilirubin UDP-glucuronyltransferase (54 kDa) was specially induced by treatment of the rats with clofibrate. This isoenzyme was not detectable in liver microsomal extracts from congenitally jaundiced Gunn rats and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80949-2
更新日期:1985-04-08 00:00:00
abstract::A monoclonal antibody, MANDYS141, binds to both dystrophin and actinin on Western blots (SDS-denatured), but only to actinin in frozen sections of human muscle (native conformation). It differs from a polyclonal cross-reacting antiserum in that it binds to several muscle isoforms of actinin (smooth, fast and slow) fro...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80460-z
更新日期:1990-10-15 00:00:00
abstract::The two cytoskeletal proteins VASP and WASP and the protein Homer share a conserved domain, currently designated the WHI domain (WASP homology domain 1) or EVH1 domain (ENA/VASP homology domain 1), which could play an important role in various cellular events such as transport, folding of proteins, and signal transduc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01541-5
更新日期:1998-12-18 00:00:00
abstract::Using the yeast two hybrid system and overlay assays we identified a putative rholrac effector, citron, which interacts with the GTP-bound forms of rho and rac1, but not with cdc42. Extensive homologies to known proteins were not observed. This 183 kDa protein contains a C6H2 zinc finger, a PH domain, and a long coile...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01351-2
更新日期:1995-12-18 00:00:00
abstract::Limb girdle muscular dystrophy type 2A is linked to a skeletal muscle-specific calpain isoform known as p94. Isolation of the intact 94-kDa enzyme has been difficult to achieve due to its rapid autolysis, and uncertainty has arisen over its Ca2+-dependence for activity. We have expressed a C-terminally truncated form ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03722-5
更新日期:2002-12-18 00:00:00
abstract::We show here that phosphorylation of the insulin receptor and insulin receptor substrate-1 is increased when suspended cells are replated on fibronectin. This is not due to decreased numbers of cell surface receptors, alteration of insulin binding, or stimulation of a phosphatase activity in non-adherent cells. Expres...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02981-7
更新日期:2001-11-02 00:00:00
abstract::Vascular endothelial cells and neutrophils synthesize and release potent vasodilatatory factors, i.e. endothelium-derived relaxing factors (EDRF) and neutrophil-derived relaxing factors (NDRF). One EDRF has been identified as nitric oxide (NO) derived from arginine. We studied the synthesis and release of NO from huma...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80562-9
更新日期:1989-02-27 00:00:00
abstract::PDZ domains are thought to act as protein-binding modules mediating the clustering of membrane and membrane-associated proteins. The INAD protein has been shown to interact via a PDZ domain with the calcium channel TRP which contributes to capacitative calcium entry into Drosophila photoreceptor cells. We have cloned ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00877-6
更新日期:1997-08-18 00:00:00
abstract::Caloric restriction (CR) is known to promote longevity in various species. Sirtuin-mediated deacetylation has been shown to be related to the promotion of longevity in some species. Here, we show that CR of rats led to an increase in the level of Werner syndrome protein (WRN), a recognized DNA repair protein. In addit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.06.031
更新日期:2008-07-23 00:00:00
abstract::Pore-forming toxins, such as colicin A, are water-soluble proteins that insert into lipid bilayers. The water-soluble structure of Colicin A is known at a high resolution and this review describes the kinetic and structural steps involved in its soluble-to-membrane bound transformation. ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(92)80895-n
更新日期:1992-07-27 00:00:00
abstract::The oxidoreductase ERp57 is a component of the major histocompatibility complex (MHC) class I peptide-loading complex. ERp57 can interact directly with MHC class I molecules, however, little is known about which of the cysteine residues within the MHC class I molecule are relevant to this interaction. MHC class I mole...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.04.034
更新日期:2007-05-15 00:00:00
abstract::Two major embryonic myosin heavy chains are expressed in embryonic chick breast muscle until the first week after hatching. Of these, one is already detected in the 8-day-old embryo. The other appears on day 12. Two putative slow embryonic isoforms represent minor components transiently expressed between days 8 and 12...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80488-5
更新日期:1988-10-10 00:00:00
abstract::We employed cDNA representational difference analysis to identify new genes that are upregulated by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in a human hepatoblastoma cell line HepG2. We isolated several TCDD-responsive cDNAs. Sequence analysis revealed that one of them encodes E16/CD98LC/hLAT1, an integral membrane...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01574-4
更新日期:1999-12-03 00:00:00
abstract::Many extracellular stimuli, e.g. microbial products, cytokines etc., result in the expression of inducible nitric oxide synthase (iNOS) in macrophages. However, it is not known whether expression of the iNOS gene in response to microbial products is a primary response of macrophages, or is the result of paracrine/auto...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.09.009
更新日期:2010-10-08 00:00:00
abstract::The aim of this work was to study the effect of a sustained activation of AMP-activated protein kinase (AMPK) on liver cell survival. AMPK activation was achieved by incubating FTO2B cells with AICA-riboside, which is transformed into ZMP, an AMP analogue, or by adenoviral transfection of hepatocytes with a constituti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03110-1
更新日期:2002-08-28 00:00:00
abstract::Oligonucleotide probes based on the known amino acid sequence of salmon calcitonin were used to screen a cDNA library obtained from ultimobranchial glands of salmon for clones encoding salmon calcitonin. From the cDNA sequence of strongly hybridizing clones the complete primary structure of the calcitonin precursor co...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80558-6
更新日期:1987-12-21 00:00:00
abstract::We used a xenograft model to investigate whether the aryl hydrocarbon receptor deletion construct CDelta553 suppresses tumor growth. HeLa cells that were infected with CDelta553 expressing adenovirus (Ad553) formed very small tumors whereas the control adenovirus-infected cells formed large tumors at day 15. CDelta553...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.08.013
更新日期:2009-09-17 00:00:00
abstract::Genetic factors seem to play a significant role in susceptibility to systemic lupus erythematosus (SLE). We previously described the amino acid polymorphism (Val14Met) within the IFN-gamma receptor 1 (IFN-gammaRI), and that the frequency of the Metl4 allele in SLE patients was significantly higher than that of the hea...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00701-2
更新日期:1999-06-18 00:00:00