Abstract:
:The aim of this work was to study the effect of a sustained activation of AMP-activated protein kinase (AMPK) on liver cell survival. AMPK activation was achieved by incubating FTO2B cells with AICA-riboside, which is transformed into ZMP, an AMP analogue, or by adenoviral transfection of hepatocytes with a constitutively active form of AMPK. Prolonged AMPK activation triggered apoptosis and activated c-Jun N-terminal kinase (JNK) and caspase-3. Experiments with iodotubercidin, dicoumarol and z-VAD-fmk, which inhibited AMPK, JNK and caspase activation, respectively, supported the notion that prolonged AMPK activation in liver cells induces apoptosis through an activation pathway that involves JNK and caspase-3.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Meisse D,Van de Casteele M,Beauloye C,Hainault I,Kefas BA,Rider MH,Foufelle F,Hue Ldoi
10.1016/s0014-5793(02)03110-1keywords:
subject
Has Abstractpub_date
2002-08-28 00:00:00pages
38-42issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579302031101journal_volume
526pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Using an all-atom, molecular dynamics-based, flexible docking method, the tertiary and quaternary structures of protofilaments of the "K3" fragment from beta(2)-microglobulin (residues Ser20-Lys41) were predicted at low pH in a continuous mixture of water and 2,2,2-trifluoroethanol (TFE). Tetramers with energies very ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.10.022
更新日期:2006-11-13 00:00:00
abstract::Epidemiological studies testing the effect of beta-carotene in humans have found a relative risk for lung cancer in smokers supplemented with beta-carotene. We investigated the reactions of retinal and beta-apo-8'-carotenal, two beta-carotene oxidation products, with 2'-deoxyguanosine to evaluate their DNA damaging po...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(04)00084-5
更新日期:2004-02-27 00:00:00
abstract::The level of reduction of cytochrome a and CuA during the oxidation of ferrocytochrome c has been determined in stopped-flow experiments. Both components are partially reduced but become progressively more oxidized as the reaction proceeds. When all cytochrome c has been oxidized, CuA is also completely oxidized, wher...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80040-0
更新日期:1986-01-01 00:00:00
abstract::The rate at which dinucleoside phosphates are cleaved by RNases is supposed to be determined by the mole fraction of enzyme-substrate complexes in which the phosphodiester moiety of a dinucleoside phosphate has a highly reactive conformation. The mole fraction of such complexes for a particular RNase depends on the na...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80521-4
更新日期:1985-01-07 00:00:00
abstract::The four chain agglutinins from Abrus precatorius, Viscum album and Ricinus communis promote depurination of the 28 S rRNA from rabbit reticulocyte ribosomes characteristic of the common ribosome-inactivating proteins (RIPs). These agglutinins inhibited mammalian protein synthesis at nanomolar concentrations but they ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80193-x
更新日期:1993-08-23 00:00:00
abstract::The Xenopus laevis oocyte has endogenous sites that bind muscarinic agonists, which have been pharmacologically characterized as M3 and/or M1 receptor subtypes. In order to define the molecular identify of the receptor protein we have analyzed a Xenopus oocyte cDNA library and cloned a 2.9 kb cDNA fragment encoding a ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00957-0
更新日期:1994-09-26 00:00:00
abstract::The differential scanning microcalorimetry and fluorescence methods, using probes ANS and pyrene, have been employed to study thermotropic behaviour of rat liver microsomes in the presence and absence of Mg2+. Addition of Mg2+ yields three partially reversible phase transitions at 18, 27 and 32 degrees C, respectively...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80295-6
更新日期:1983-12-12 00:00:00
abstract::CDK11(p58), a 58kDa protein of the PITSLRE kinase family, plays an important role in cell cycle progression, and is closely related to cell apoptosis. To gain further insight into the function of CDK11(p58), we screened a human fetal liver cDNA library for its interacting proteins using the yeast two-hybrid system. He...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.05.039
更新日期:2005-07-04 00:00:00
abstract::Two hexokinases were characterized in Schizosaccharomyces pombe: hexokinase 1, with a low phosphorylation coefficient on glucose (Km 8.5 mM) and hexokinase 2, a kinetically conventional hexokinase. Genes hxk1+ and hxk2+ encoding these enzymes were cloned and sequenced. Disruption of hxk1+ had no effect on growth but d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01451-9
更新日期:1996-01-08 00:00:00
abstract::The actin-depolymerising factor (ADF)/cofilin group of proteins are stimulus-responsive actin-severing proteins, members of which are regulated by reversible phosphorylation. The phosphorylation site on the maize ADF, ZmADF3, is Ser-6 but the kinase responsible is unknown [Smertenko et al., Plant J. 14 (1998) 187-193]...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02528-5
更新日期:2001-06-15 00:00:00
abstract::Bacillus subtilis heme A synthase is a membrane protein with 8 transmembrane segments. By using a two-step mutagenesis approach we have generated and selected a fully functional enzyme protein variant with a seven residue internal deletion. The biochemical properties of the shortened variant are similar to those of th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.03.015
更新日期:2008-04-16 00:00:00
abstract::The adiponectin paralog CORS-26 (collagenous repeat-containing sequence of 26kDa protein) is a member of the C1q/TNF-alpha molecular superfamily. CORS-26 is a secreted protein and baculovirus-produced CORS-26 released in the supernatant of insect cells forms stable trimers. Adiponectin exerts anti-inflammatory effects...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.09.022
更新日期:2005-10-24 00:00:00
abstract::The final step in the biosynthesis of nicotinamide-adenine dinucleotide, a major coenzyme in cellular redox reactions and involved in intracellular signaling, is catalyzed by the enzyme nicotinamide mononucleotide adenylyltransferase (NMNAT). The X-ray structure of human NMNAT in complex with nicotinamide mononucleoti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02556-5
更新日期:2002-04-10 00:00:00
abstract::In PC12 cells, cAMP stimulates the MAP kinase pathway by an unknown mechanism. Firstly, we examined the role of calcium ion mobilization and of protein kinase C in cAMP-stimulated MAP kinase activation. We show that cAMP stimulates p44mapk independently of these events. Secondly, we studied the role of B-Raf in this p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01376-c
更新日期:1995-01-09 00:00:00
abstract::The specific binding of [3H]retinoids to cellular retinoid-binding proteins was measured directly by the cold acetone filtration method. After incubation of purified cellular retinoid-binding proteins with [3H]retinoids with or without competitors for 2-4 h, bound ligands were separated from free by filtration using c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80437-x
更新日期:1988-06-20 00:00:00
abstract::A Synechococcus PCC7942 mutant in which the psbO gene was inactivated by insertion of a chloramphenicol interposon and which did not contain any detectable manganese stabilizing protein in immunoblot experiments, was constructed. Such a Synechococcus mutant was able to grow under photoautotrophic conditions. Isolated ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81343-7
更新日期:1991-12-02 00:00:00
abstract::In freshly prepared low density lipoprotein (LDL), ascorbate inhibited LDL oxidation by macrophages at the higher concentrations tested (60-100 microM). In contrast, with LDL that had been allowed to autoxidise in the refrigerator (3 degrees C) for at least 10 weeks after isolation (mildly oxidised or minimally-modifi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80469-9
更新日期:1994-03-21 00:00:00
abstract::The fluorescence intensity of the Ca2+,Mg2(+)-ATPase protein of rabbit skeletal sarcoplasmic reticulum that incorporated about 2 mol of 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) was enhanced at high MgATP concentrations with or without 50 microM calcium. The observed enhancement indicates that the fluorophore, ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80021-a
更新日期:1990-01-01 00:00:00
abstract::In the methylerythritol phosphate pathway for isoprenoid biosynthesis, the GcpE/IspG enzyme catalyzes the conversion of 2-C-methyl-d-erythritol 2,4-cyclodiphosphate into (E)-4-hydroxy-3-methylbut-2-enyl diphosphate. This reaction requires a double one-electron transfer involving a [4Fe-4S] cluster. A thylakoid prepara...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.01.082
更新日期:2006-03-06 00:00:00
abstract::We found that, in the presence of chimeric oligonucleotides containing complementary deoxyribo- and 2'-O-methylnucleosides, a nonaribonucleotide, [5'-32P]pACUUACCUG, was cleaved specifically upon treatment with RNase H. When 3'm(UG)d(AATG)m(GAC)5' was used as a hybridization strand, pACUUACCUG was cleaved between C6 a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80171-0
更新日期:1987-05-11 00:00:00
abstract::Temperature-jump measurements were carried out on myosin subfragment 1 (S1) labeled at Cys-707 with 5-(iodoacetamido)fluorescein (S1-AF). The relaxation was monitored by following the increase in the fluorescence intensity of the attached probe after a jump of 5.8 degrees C. A single relaxation process was observed ov...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80614-m
更新日期:1992-06-15 00:00:00
abstract::Following the biophysical analysis of plant K+ channels in their natural environment, three members from the green branch of the evolutionary tree of life KAT1, AKT1, and KST1 have recently been identified on the molecular level. Among them, we focussed on the expression and characterization of the Arabidopsis thalian...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00042-7
更新日期:1996-02-19 00:00:00
abstract::Ribosomal protection proteins (RPPs) confer bacterial resistance to tetracycline by releasing this antibiotic from ribosomes stalled in protein synthesis. RPPs share structural similarity to elongation factor G (EF-G), which promotes ribosomal translocation during normal protein synthesis. We constructed and functiona...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.03.023
更新日期:2008-04-16 00:00:00
abstract::In Alzheimer disease (AD) the microtubule associated protein (MAP) tau is hyperphosphorylated at several sites. In the present study, like AD tau, tau in the human neuroblastoma SH-SY5Y was found to be hyperphosphorylated, at Ser-199/202, Thr-231, Ser-396 and Ser-404. However, in contrast to AD, the tau in SY5Y cells ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00061-d
更新日期:1995-02-20 00:00:00
abstract::Actin filaments copolymerized with both intact and chemically modified actin monomers restored their sliding activity when they were supplemented with tropomyosin extracted from skeletal muscle. In contrast, the ATPase activation of the copolymers was decreased when supplemented with tropomyosin. The results indicate ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00218-9
更新日期:1996-03-25 00:00:00
abstract::The cardiac and skeletal muscle isoforms of calsequestrin (CS), the low affinity, high capacity Ca2+ binding protein localized in the lumen of sarcoplasmic reticulum, are the products of two different genes (Fliegel, L., Leberer, E., Green, N.M. and MacLennan, D.H. (1982) FEBS Lett. 242, 297-300), and can be both puri...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80241-8
更新日期:1992-03-09 00:00:00
abstract::Buthionine sulfoximine (BSO) induces decreased glutathione (GSH) and trypanothione [T(SH)2 ] pools in trypanosomatids, presumably because only gamma-glutamylcysteine synthetase (γECS) is blocked. However, some BSO effects cannot be explained by exclusive γECS inhibition; therefore, its effect on the T(SH)2 metabolism ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12904
更新日期:2017-12-01 00:00:00
abstract::Stimulated production of reactive oxygen species (ROS) by plasma membrane-associated nicotinamide adenine dinucleotide phosphate oxidases (Nox) in non-phagocytic cells regulates a number of biological processes including growth, vessel tone, and oxygen sensing. The purpose of this study was to investigate H(2)O(2)-sti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.03.057
更新日期:2005-04-25 00:00:00
abstract::PGI is a housekeeping gene encoding phosphoglucose isomerase (PGI) a glycolytic enzyme that also functions as a cytokine (autocrine motility factor (AMF)/neuroleukin/maturation factor) upon secretion from the cell and binding to its 78 kDa seven-transmembrane domain receptor (gp78/AMF-R). PGI contains a CXXC motif, ch...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-6793(02)03773-0
更新日期:2003-01-16 00:00:00
abstract::The 27 kDa platelet membrane protein (Gn27) that binds [alpha-32P]GTP on nitrocellulose blots of SDS-polyacrylamide gels [(1987) Biochem. J. 245, 617-620] was compared with other low molecular mass GTP-binding proteins. Platelet membranes also contained 21 kDa proteins that bound anti-ras p21 antibody and 22-23 kDa pr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80194-7
更新日期:1988-09-12 00:00:00