Abstract:
:Epidemiological studies testing the effect of beta-carotene in humans have found a relative risk for lung cancer in smokers supplemented with beta-carotene. We investigated the reactions of retinal and beta-apo-8'-carotenal, two beta-carotene oxidation products, with 2'-deoxyguanosine to evaluate their DNA damaging potential. A known mutagenic adduct, 1,N(2)-etheno-2'-deoxyguanosine, was isolated and characterized on the basis of its spectroscopic features. After treatment of calf thymus DNA with beta-carotene or beta-carotene oxidation products, significantly increased levels of 1,N(2)-etheno-2'-deoxyguanosine and 8-oxo-7,8-dihydro-2'-deoxyguanosine were quantified in DNA. These lesions are believed to be important in the development of human cancers. The results reported here may contribute toward an understanding of the biological effects of beta-carotene oxidation products.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Marques SA,Loureiro AP,Gomes OF,Garcia CC,Di Mascio P,Medeiros MHdoi
10.1016/S0014-5793(04)00084-5keywords:
subject
Has Abstractpub_date
2004-02-27 00:00:00pages
125-30issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579304000845journal_volume
560pub_type
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