Abstract:
:We have previously shown that the Mycoplasma mycoides glycine tRNA (anticodon UCC) effectively reads the codons GGU and GGC in violation of the classic codon reading rules. We have attempted to elucidate what structural elements in this tRNA molecule confer this translational property and in the course of this investigation T7 RNA polymerase transcription of the corresponding gene was used to produce a tRNA devoid of modified nucleosides. Using an in vitro translation system the ability of this tRNA to read the 4 glycine codons (GGU, GGC, and GGG) was tested and it was shown to be as efficient as its normal, fully modified counterpart in the reading of all four codons. This result demonstrates that a tRNA devoid of modified nucleosides is able to efficiently sustain protein synthesis in vitro and, furthermore, that the normal modification pattern of the Mycoplasma glycine tRNA is not essential for the ability of this tRNA to read the glycine codons GGU and GGC effectively.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Claesson C,Samuelsson T,Lustig F,Borén Tdoi
10.1016/0014-5793(90)81077-2subject
Has Abstractpub_date
1990-10-29 00:00:00pages
173-6issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(90)81077-2journal_volume
273pub_type
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