Abstract:
:It was determined that flavan-3-ols and procyanidins have an inhibitory effect on angiotensin I converting enzyme (ACE) activity, and the effect was dependent on the number of epicatechin units forming the procyanidin. The inhibition by flavan-3-ols and procyanidins was competitive with the two substrates assayed: N-hippuryl-L-histidyl-L-leucine (HHL) and N-[3-(2-furyl)acryloyl]-L-phenylalanylglycylglycine (FAPGG). Tetramer and hexamer fractions were the more potent inhibitors, showing Ki of 5.6 and 4.7 microM, respectively. As ACE is a membrane protein, the interaction of flavanols and procyanidins with the enzyme could be related to the number of hydroxyl groups on the procyanidins, which determine their capacity to be adsorbed on the membrane surface.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Actis-Goretta L,Ottaviani JI,Keen CL,Fraga CGdoi
10.1016/s0014-5793(03)01355-3keywords:
subject
Has Abstractpub_date
2003-12-18 00:00:00pages
597-600issue
3eissn
0014-5793issn
1873-3468pii
S0014579303013553journal_volume
555pub_type
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