ADP-ribosylation of metaphase and interphase nonhistones using [3H]adenosine as a radioactive label.

abstract：:LH-stimulated adenylate cyclase activity in membrane preparations of bovine luteal cells could be enhanced by treating the cells with either phospholipase D or its hydrolysis product, phosphatidic acid. Similar augmentary effects were also produced following treatment of the cells with EGF. Moreover, EGF could stimula...

journal_title：FEBS letters

authors： Budnik LT,Mukhopadhyay AK

更新日期：1993-07-12 00:00:00

abstract：:The retromer complex is a key element of the endosomal protein sorting machinery that is conserved through evolution and has been shown to play a role in diseases such as Alzheimer's disease and Parkinson's disease. Through sorting various membrane proteins (cargo), the function of retromer complex has been linked to ...

journal_title：FEBS letters

authors： Mukadam AS,Seaman MN

更新日期：2015-09-14 00:00:00

abstract：:Apomyoglobin is a widely used model for studying the molecular mechanisms of globular protein folding. This work aimed to analyze the effects of rigidity and length of loops linking protein secondary structure elements on the stability of the molten globule intermediate state. For this purpose, we studied folding/unfo...

journal_title：FEBS letters

authors： Majorina MA,Balobanov VA,Uversky VN,Melnik BS

更新日期：2020-10-01 00:00:00

abstract：:Accumulation of hyperphosphorylated Tau is associated with a number of neurodegenerative diseases collectively known as tauopathies. Differences in clinical and cognitive profiles among them suggest differential sensitivity of neuronal populations to Tau levels, phosphorylation and mutations. We used tissue specific e...

journal_title：FEBS letters

authors： Grammenoudi S,Kosmidis S,Skoulakis EM

更新日期：2006-08-21 00:00:00

abstract：:A poly(A) tail functions in mRNA turnover and in facilitating translation as a ribonucleoprotein complex with poly(A) binding proteins (PABPs). However, factors that associate with the poly(A) tail other than PABPs have not been described. Using proteomics, we identified candidate proteins that interact to the 3' term...

journal_title：FEBS letters

authors： Aoki K,Adachi S,Homoto M,Kusano H,Koike K,Natsume T

更新日期：2013-07-11 00:00:00

abstract：:A variety of mutations leading to amino acid substitutions have been described in the transthyretin gene in association with different familial amyloidoses and have been implicated to be involved in the pathogenesis of amyloid deposits. However, there has been disagreement whether or not a transthyretin mutation is pr...

journal_title：FEBS letters

authors： Christmanson L,Betsholtz C,Gustavsson A,Johansson B,Sletten K,Westermark P

更新日期：1991-04-09 00:00:00

abstract：:Addition of hydrocortisone to the rat mammary gland myoepithelial cell line, G4.2.3, induces the synthesis and secretion of alpha 2-macroglobulin. Interleukin-1 beta, interleukin-6 and transforming growth factor-beta synergize with hydrocortisone, increasing the synthesis of alpha 2-macroglobulin 2- to 4-fold, althoug...

journal_title：FEBS letters

authors： Warburton MJ,Gusterson BA,O'Hare MJ

更新日期：1993-10-11 00:00:00

abstract：:A new insertion sequence, IS186, has been identified from Escherichia coli and sequenced. It contains 1336 base pairs with a terminal inverted repeat of 22 nucleotides. A long open reading frame, from an ATG codon at position 55 to a TAG termination codon at 1327, could code for a polypeptide of 424 amino acids. This ...

journal_title：FEBS letters

authors： Chong P,Hui I,Loo T,Gillam S

更新日期：1985-11-11 00:00:00

abstract：:A collagenase cleaving native type I [14C]collagen but inactive against the synthetic substrate Pz-Pro-Leu-Gly-Pro-D-Arg was extracted from mineralized human dental tissue. The enzyme specifically degrades native collagen into characteristic products (3/4) and (1/4). Its apparent molecular mass of 68 kDa is relatively...

journal_title：FEBS letters

authors： Dumas J,Hurion N,Weill R,Keil B

更新日期：1985-07-22 00:00:00

abstract：:The selectively red excited emission spectrum, at room temperature, of the in vitro reconstituted Lhca4, has a pronounced non-equilibrium distribution, leading to enhanced emission from the directly excited low-energy pigments. Two different emitting forms (or states), with maximal emission at 713 and 735nm (F713 and ...

journal_title：FEBS letters

authors： Zucchelli G,Morosinotto T,Garlaschi FM,Bassi R,Jennings RC

更新日期：2005-04-11 00:00:00

abstract：:Calpastatin molecule contains four repeated inhibition domains, each having highly conserved internal regions A, B and C. The synthetic oligopeptides of regions A and C had no calpain inhibition activity while region B oligopeptide showed weak inhibition activity. Real-time biomolecular interaction analysis using a BI...

journal_title：FEBS letters

authors： Takano E,Ma H,Yang HQ,Maki M,Hatanaka M

更新日期：1995-03-27 00:00:00

abstract：:We have investigated the availability of zinc in the periplasmic space of Escherichia coli using a mutant Cu,Zn superoxide dismutase whose dimerization is triggered by zinc binding. This mutant enzyme accumulates in the monomeric form when wild type cells are grown in minimal medium, but assembles in the dimeric form ...

journal_title：FEBS letters

authors： Berducci G,Mazzetti AP,Rotilio G,Battistoni A

更新日期：2004-07-02 00:00:00

abstract：:Circadian clocks are endogenous timing systems orchestrating the daily regulation of a huge variety of physiological, metabolic and behavioral processes. These clocks are important for health - in mammals, their disruption leads to a diverse number of pathologies. While genetic and biochemical approaches largely uncov...

journal_title：FEBS letters

authors： Wallach T,Kramer A

更新日期：2015-06-22 00:00:00

abstract：:The effect of artificial model membranes on the complement system was investigated. Incubation of the model membranes with human serum resulted in consumption of complement haemolytic activity when phosphatidylserine-containing vesicles were used. The activation of the complement system appeared to proceed through the...

journal_title：FEBS letters

authors： Comis A,Easterbrook-Smith SB

更新日期：1986-03-03 00:00:00

abstract：:Cyclic AMP (cAMP) and its action by way of cAMP-dependent protein kinase is important for sperm motility. Previous studies on germ cells have demonstrated a selective decrease in the amount of type I cAMP-dependent protein kinase during spermatid development, and that type II was the major form present in elongating s...

journal_title：FEBS letters

authors： Oyen O,Scott JD,Cadd GG,McKnight GS,Krebs EG,Hansson V,Jahnsen T

更新日期：1988-03-14 00:00:00

abstract：:We previously isolated a Saccharomyces cerevisiae mutant (HsTnII), which displays 40% reduced chronological lifespan as compared to the wild type (WT). In this study, we found HsTnII cultures to be characterized by fragmented and dysfunctional mitochondria, and by increased initiation of apoptosis during chronological...

journal_title：FEBS letters

authors： Aerts AM,Zabrocki P,Govaert G,Mathys J,Carmona-Gutierrez D,Madeo F,Winderickx J,Cammue BP,Thevissen K

更新日期：2009-01-05 00:00:00

abstract：:Coronin proteins are widely expressed among eukaryotic organisms. Most coronins consist of a WD-repeat domain followed by a C-terminal coiled coil. Dictyostelium discoideum expresses a single short coronin coronin A, which has been implicated in both actin modulation and multicellular differentiation. Whether coronin ...

journal_title：FEBS letters

authors： Fiedler T,Fabrice TN,Studer V,Vinet A,Faltova L,Kammerer RA,Steinmetz MO,Sharpe T,Pieters J

更新日期：2020-04-16 00:00:00

abstract：:Temperature-jump measurements were carried out on myosin subfragment 1 (S1) labeled at Cys-707 with 5-(iodoacetamido)fluorescein (S1-AF). The relaxation was monitored by following the increase in the fluorescence intensity of the attached probe after a jump of 5.8 degrees C. A single relaxation process was observed ov...

journal_title：FEBS letters

authors： Lin SH,Cheung HC

更新日期：1992-06-15 00:00:00

abstract：:Phospholipase D was first described in plant tissue but has recently been shown to occur in mammalian cells where it is activated by cell surface receptors. Its mode of activation by receptors in unclear. Biochemical studies suggest that it may occur downstream of other effector proteins and that small GTP-dependent r...

journal_title：FEBS letters

authors： Holbrook PG,Geetha V,Beaven MA,Munson PJ

更新日期：1999-04-09 00:00:00

abstract：:Lactate dehydrogenase (LDH) and yeast alcohol dehydrogenase ( YADH ) are inactivated when attacked by hydroxy free radicals (OH). Organic molecules with a high rate constant of reaction with OH such as ascorbate or urate can compete with the enzymes for these strongly oxidising radicals. However, although 10(-3)M asco...

journal_title：FEBS letters

authors： Kittridge KJ,Willson RL

更新日期：1984-05-07 00:00:00

abstract：:Intracellular vesicular transport involves targeting of vesicles to their correct destination and subsequent fusion with the target membrane. These processes employ unique compartment-specific proteins in combination with general components which act at several transport steps. This review will summarize recent progre...

journal_title：FEBS letters

authors： Söllner T

更新日期：1995-08-01 00:00:00

abstract：:The effect of the non-steroidal antioestrogens tamoxifen and toremifene on voltage-gated cationic currents was examined in primary cultures of rat hypothalamic neurones and the C1300 mouse neuroblastoma cell line using the whole-cell patch clamp technique. When applied to the external bathing solution both tamoxifen a...

journal_title：FEBS letters

authors： Hardy SP,deFelipe C,Valverde MA

更新日期：1998-09-04 00:00:00

abstract：:Cyclic ADP-ribose (cADPR), an endogenous NAD+ metabolite in many mammalian and invertebrate tissues, is a potent mediator of calcium mobilization in sea urchin eggs. Our results show that cADPR also stimulates calcium release from rat brain microsomes, marked release occurring over the concentration range 10-250 nM. T...

journal_title：FEBS letters

authors： White AM,Watson SP,Galione A

更新日期：1993-03-08 00:00:00

abstract：:RNA binding motif protein 5 (RBM5) is a candidate tumor suppressor gene. Recent studies showed that RBM5 functions as an alternative splicing regulator of apoptosis-related genes. Here, we identify DHX15 and PRP19, two spliceosome components, as novel RBM5-interacting partners. We then show that the G-patch domain of ...

journal_title：FEBS letters

authors： Niu Z,Jin W,Zhang L,Li X

更新日期：2012-04-05 00:00:00

abstract：:Understanding protein beta structures has been hindered by the challenge of designing small, well-folded β-sheet systems. A β-capping motif was previously designed to help solve this problem, but not without limitations, as the termini of this β-cap were not fully available for chain extension. Combining Coulombic sid...

journal_title：FEBS letters

authors： Anderson JM,Kier BL,Shcherbakov AA,Andersen NH

更新日期：2014-12-20 00:00:00

abstract：:Subcellular fractionation revealed that a significant fraction of total sphingosine kinase, the enzyme that phosphorylates sphingosine to form the bioactive lipid metabolite sphingosine-1-phosphate, resides in the nuclei of Swiss 3T3 cells, localized to both the nuclear envelope and the nucleoplasm. Platelet-derived g...

journal_title：FEBS letters

authors： Kleuser B,Maceyka M,Milstien S,Spiegel S

更新日期：2001-08-10 00:00:00

abstract：:Degradation of extracellular matrix proteins is performed by metalloproteinases which are inhibited by tissue inhibitors of metalloproteinases (TIMP). We expressed the murine TIMP-1 protein in E. coli and prepared a polyclonal antiserum against the recombinant protein. Using this antiserum we studied the biosynthesis ...

journal_title：FEBS letters

authors： Roeb E,Graeve L,Müllberg J,Matern S,Rose-John S

更新日期：1994-07-25 00:00:00

abstract：:10Sa RNA is a bacterial small stable RNA, in which the 5'- and 3'-end sequences are folded into a tRNA-like structure. The RNA accepts alanine in vitro, and interacts with 70S ribosomes in the cells. In this study, we examined the ribosome-binding properties of Escherichia coli 10Sa RNA in vivo, and found that the ami...

journal_title：FEBS letters

authors： Tadaki T,Fukushima M,Ushida C,Himeno H,Muto A

更新日期：1996-12-16 00:00:00

abstract：:The binding of L-[35S]methionine in vivo labelled CryIC toxin to its receptor in brush border membrane vesicle (BBMV's) prepared from Spodoptera littoralis and Bombyx mori was studied. Both insect species were highly susceptible to the CryIC toxin in bioassays, B. mori being 7-fold more sensitive to CryIC than S. litt...

journal_title：FEBS letters

authors： Sanchis V,Chaufaux J,Pauron D

更新日期：1994-10-24 00:00:00

abstract：:Higher basal 2-deoxy-D-glucose uptake in rheumatoid synovial cells than in non-rheumatoid synovial cells, was found to be associated with an increased interleukin-1 beta (IL-1 beta) secretion (respectively 850 +/- 238 vs. 8.3 +/- 2.4 pg/24 h/10(5) cells, mean +/- S.E.M.). When exogenous human recombinant IL-1 beta was...

journal_title：FEBS letters

authors： Hernvann A,Aussel C,Cynober L,Moatti N,Ekindjian OG

更新日期：1992-05-25 00:00:00