Abstract:
:The binding of L-[35S]methionine in vivo labelled CryIC toxin to its receptor in brush border membrane vesicle (BBMV's) prepared from Spodoptera littoralis and Bombyx mori was studied. Both insect species were highly susceptible to the CryIC toxin in bioassays, B. mori being 7-fold more sensitive to CryIC than S. littoralis (LC50's of 10 ng/cm2 and 70 ng/cm2, respectively). Competition and direct binding experiments revealed saturable high-affinity binding sites on BBMV's from both insects which had similar binding characteristics for the CryIC toxin (Kd = 10 nM, Bmax = 8 to 9 pmol/mg BBMV's and IC50 = 37 nM for both inspect species). Thus a specific receptor for the CryIC toxin is present in both insect species and the 7-fold greater potency of CryIC towards B. mori is not due to qualitative or quantitative differences in binding affinity or receptor site concentration. Dissociation experiments also indicated that the binding of [35S]CryIC to B. mori BBMV's is partially reversible.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sanchis V,Chaufaux J,Pauron Ddoi
10.1016/0014-5793(94)01057-9subject
Has Abstractpub_date
1994-10-24 00:00:00pages
259-63issue
3eissn
0014-5793issn
1873-3468journal_volume
353pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::In the mouse macrophage-like cell line RAW 264, vacuolar-type (H(+))-ATPase (V-ATPase) inhibitors, bafilomycin A(1) and concanamycin A, increased the level of cyclooxygenase (COX)-2 protein and its mRNA. The V-ATPase inhibitor-induced expression of COX-2 was suppressed by inhibitors of c-jun N-terminal kinase (JNK) an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.08.055
更新日期:2007-10-02 00:00:00
abstract::Myeloperoxidase (MPO) is the most abundant protein in neutrophils and plays a central role in microbial killing and inflammatory tissue damage. Because most of the non-steroidal anti-inflammatory drugs and other drugs contain a thiol group, it is necessary to understand how these substrates are oxidized by MPO. We hav...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01727-x
更新日期:1999-01-29 00:00:00
abstract::Protamines are polycationic proteins that are widely used for neutralisation of the anticoagulant action of heparin. However, several reports have shown adverse, mast cell-dependent reactions to protamine. The exact mechanism by which protamine causes these adverse effects is not clear. In the present study, the possi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00239-6
更新日期:1996-04-01 00:00:00
abstract::The last enzyme (LytB) of the methylerythritol phosphate pathway for isoprenoid biosynthesis catalyzes the reduction of (E)-4-hydroxy-3-methylbut-2-enyl diphosphate into isopentenyl diphosphate and dimethylallyl diphosphate. This enzyme possesses a dioxygen-sensitive [4Fe-4S] cluster. This prosthetic group was charact...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00317-x
更新日期:2003-04-24 00:00:00
abstract::We have determined the first de novo position of the secondary quinone QB in the Rhodobacter sphaeroides reaction center (RC) using phases derived by the single wavelength anomalous dispersion method from crystals with selenomethionine substitution. We found that in frozen RC crystals, QB occupies primarily the proxim...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.06.042
更新日期:2004-07-16 00:00:00
abstract::In this study we used the fungal antibiotic brefeldin A (BFA) to analyze its effect on viral replication. Analysis by electron microscopy demonstrated that no viral particles were observed in cells treated before the onset of viral replication. In the presence of BFA expression of IE2, MCP, pUL104, pUL56 and pUL89 wer...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.03.011
更新日期:2009-04-02 00:00:00
abstract::Two protein phosphatases of 103 and 29 kDa as determined by gel filtration, were purified from hen oviducts. The 103 -kDa phosphatase was purified 7300-fold to near homogeneity and dissociated into two polypeptides in the presence of SDS. Molecular masses of these polypeptides were estimated to be 60 and 38 kDa by SDS...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80657-8
更新日期:1985-05-06 00:00:00
abstract::Three proteinase inhibitor genes have been identified in the rapeseed (Brassica napus) genome. They are highly homologous to other genes of the mustard inhibitor (MSI) family of proteinase inhibitors characteristic of Cruciferae. In germinating seeds, only the transcript of one gene, coding for a trypsin inhibitor, is...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.01.022
更新日期:2006-02-06 00:00:00
abstract::Conformational changes in the extradiscal regions of rhodopsin induced by illumination were investigated by modifying the visual pigment by mild treatment with cyanogen bromide prior to and after light exposure. Light induced an increased yield of cleavage of the Met bond 253-254 and a new cleavage at the Met bond 155...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81138-8
更新日期:1985-02-11 00:00:00
abstract::Escherichia coli 50 S ribosomal subunits were reconstituted with and without protein L16 present. The latter particles, although active in puromycin reaction, were unable to use CACCA-Phe as an acceptor substrate. We also found that L16 interacts directly with this oligonucleotide and, in the complex with tRNA, protec...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80043-5
更新日期:1984-01-23 00:00:00
abstract::Vesicular stomatitis virus (VSV) is a prototypic virus commonly used in studies of endocytosis and membrane trafficking. One proposed mechanism for VSV entry involves initial fusion with internal vesicles of multivesicular endosomes followed by back-fusion of these vesicles into the cytoplasm. One feature of endosomal...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.02.015
更新日期:2011-03-23 00:00:00
abstract::A number of human diseases can be linked to aberrations in protein folding which cause an imbalance in protein homeostasis. Molecular chaperones, including heat shock proteins, act to assist protein folding, stability and activity in the cell. Attention has begun to focus on modulating the expression and/or activity o...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2007.05.040
更新日期:2007-07-31 00:00:00
abstract::The cleavable prepiece of the precursor to yeast cytochrome c oxidase subunit IV (an imported mitochondrial protein) was attached to the amino-terminus of mouse dihydrofolate reductase (a cytosolic protein) by gene fusion. The resulting fusion protein was imported into the matrix of isolated, energized yeast mitochond...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80622-5
更新日期:1984-12-10 00:00:00
abstract::Glutamate uptake by rat hepatocytes in primary monolayer culture was found to be mediated by a Na+-independent and by two Na+-dependent transport systems of high and low affinity. Inhibition studies with cysteate and other model amino acids rules out the participation of the neutral amino acid transport systems A, ASC...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)81024-2
更新日期:1983-09-19 00:00:00
abstract::We have used DNase I footprinting to examine the effect of a novel naphthylquinoline dimer, designed as a triplex-specific bis-intercalator, on the stability of intermolecular DNA triplexes. We find that this compound efficiently promotes triplex formation between the 9-mer oligonucleotide 5'-TTTTTTCTT and its oligopu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00284-7
更新日期:1999-03-26 00:00:00
abstract::(7-methoxycoumarin-4-yl)Acetyl-Pro-Leu-Gly-Leu-(3-[2,4-dinitrophenyl]-L- 2,3-diaminopropionyl)-Ala-Arg-NH2 (Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2) has been synthesised as a fluorogenic substrate for the matrix metalloproteinases. The highly fluorescent 7-methoxycoumarin group is efficiently quenched by energy transfer t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80300-6
更新日期:1992-01-27 00:00:00
abstract::To complete the amino acid sequence of GTP:AMP phosphotransferase (MgGTP + AMP in equilibrium with MgGDP + ADP) from beef heart mitochondria it was necessary to sequence an intermediate region of about 33 residues after position 102 [(1984) Eur. J. Biochem. 143, 331-339] and find a suitable overlap with the rest of th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80706-2
更新日期:1986-07-07 00:00:00
abstract::alpha-Allenyl putrescine (5,6-heptadiene-1,4-diamine) was designed as a new potential enzyme-activated irreversible inhibitor of ornithine decarboxylase (ODC). This compound, and more specifically its (R)-enantiomer, produced time-dependent inhibitions of E. coli and rat liver ODC. The inhibitions exhibit saturation k...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81172-2
更新日期:1984-09-03 00:00:00
abstract::Biological activities of five synthetic lipid A analogues (D-glucosamine derivatives) were examined to elucidate the structure required for expression of the biological activities of endotoxin. Proclotting enzyme of horseshoe crab activation, interferon-inducing and tumor necrosis factor-inducing activities were signi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80131-3
更新日期:1984-02-27 00:00:00
abstract::HslVU is an ATP-dependent protease consisting of two multimeric components, the HslU ATPase and the HslV peptidase. To gain an insight into the role of HslVU in regulation of cell division, the reconstituted enzyme was incubated with SulA, an inhibitor of cell division in Escherichia coli, or its fusion protein with m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00935-7
更新日期:1999-07-30 00:00:00
abstract::It is well known that, in rabbits, the allotypes of the b locus carried by the light chains of the IgG molecules cross-react. Nothing similar has been described for the allotypes of the a locus carried by the heavy chains of the IgG molecules. This paper describes a cross-reaction between the rabbit allotypic markers ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(69)80028-1
更新日期:1969-02-01 00:00:00
abstract::SP-40,40 is a two-chain serum protein which acts in vitro as a potent inhibitor of the assembly of the membrane attack complex of human complement. It contains 10 cysteine residues, the numbers and locations of which are conserved in several mammalian species. Evidence is presented that all the cysteine residues are i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80330-j
更新日期:1992-02-03 00:00:00
abstract::Several approaches have been used to identify the factors involved in mRNA splicing. None of them, however, comprises a straightforward reversible method for inhibiting the second step of splicing using an external reagent other than a chelator. This investigation demonstrates that the addition of boric acid to an in ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00928-1
更新日期:2003-09-25 00:00:00
abstract::ATP synthesis rate, measured as the variation in external Pi concentration, varied as a linear function of either delta mu H+ or delta Gpin, in such a manner that the delta Gpin/delta mu H+ ratio increased while VATP increased. We also observed a linear dependence of the flux control coefficient of the Pi carrier on...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80539-3
更新日期:1989-02-27 00:00:00
abstract::The STARD1 subfamily of 'START' lipid trafficking proteins can reduce macrophage lipid content and inflammatory status (STARD1; StAR), and traffic cholesterol from endosomes (STARD3/MLN64). During macrophage differentiation, STARD1 mRNA and protein increase with sterol content, while the reverse is true for STARD3. St...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.02.042
更新日期:2009-04-02 00:00:00
abstract::Evidence is presented here that cupric ions play a part in the natural spinning of Bombyx mori silk. Proton induced X-ray emission studies revealed that the copper content increased from the posterior part to the anterior part of silk gland, and then further increased in the silk fiber. Spectrophotometric analysis dem...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01184-0
更新日期:2003-11-20 00:00:00
abstract::2-( Dimethylaminostyryl )-l-methyl pyridinium, a fluorescent indicator of mitochondrial membrane potential, is accumulated up to 400-fold by intact, isolated brown adipocytes from cold-adapted guinea pigs. The indicator can be optically localized within the in situ mitochondria. Ionophores which would be predicted to ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81395-2
更新日期:1984-05-07 00:00:00
abstract::The key mechanism in prion disease is the conversion of cellular prion protein into an altered, pathogenic conformation, in which cellular mechanisms play a poorly understood role. Both forms of prion protein are lipid-anchored and reside in rafts that appear to protect the native conformation against conversion. Neur...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2006.07.053
更新日期:2006-10-09 00:00:00
abstract::Nuclear ionic channels (NICs) represent ubiquitous structures of living cells, although little is known about their functional properties and encoding genes. To characterize NICs, liver nuclear membrane vesicles were reconstituted into either planar lipid bilayers or proteoliposomes. Reconstitution of nuclear envelope...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01752-x
更新日期:2000-07-07 00:00:00
abstract::Photo-oxidized low-density lipoprotein is cytotoxic to bovine aortic endothelial cells in a concentration-dependent manner. Total cell killing occurs at a concentration of 600 mumol/l lipid hydroperoxide (LOOH). Selenium deficiency enhances the toxicity of LOOH such that 300 mumol/l LOOH is cytotoxic. This toxicity is...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00178-c
更新日期:1995-03-20 00:00:00