Abstract:
:We have used DNase I footprinting to examine the effect of a novel naphthylquinoline dimer, designed as a triplex-specific bis-intercalator, on the stability of intermolecular DNA triplexes. We find that this compound efficiently promotes triplex formation between the 9-mer oligonucleotide 5'-TTTTTTCTT and its oligopurine duplex target at concentrations as low as 0.1 microM, enhancing the triplex stability by at least 1000-fold. This compound, which is the first reported example of a triplex bis-intercalator, is about 30 times more potent than the simple monofunctional ligand.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Keppler M,Zegrocka O,Strekowski L,Fox KRdoi
10.1016/s0014-5793(99)00284-7keywords:
subject
Has Abstractpub_date
1999-03-26 00:00:00pages
223-6issue
2-3eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00284-7journal_volume
447pub_type
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