Abstract:
:2-( Dimethylaminostyryl )-l-methyl pyridinium, a fluorescent indicator of mitochondrial membrane potential, is accumulated up to 400-fold by intact, isolated brown adipocytes from cold-adapted guinea pigs. The indicator can be optically localized within the in situ mitochondria. Ionophores which would be predicted to lower or increase the in situ mitochondrial membrane potential lead to decreased and increased uptake, respectively, of the radio-labelled indicator. Noradrenaline induces a 15 mV decrease in the sum of mitochondrial and plasma membrane potentials. The results indicate that the mitochondria within isolated brown adipocytes maintain a substantial membrane potential in the resting state, rather than being artifactually depolarized.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Rafael J,Nicholls DGdoi
10.1016/0014-5793(84)81395-2subject
Has Abstractpub_date
1984-05-07 00:00:00pages
181-5issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(84)81395-2journal_volume
170pub_type
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