Abstract:
:Streptomyces lividans 1326-9, a bldA+ strain, and its bldA39 mutant derivative J1725 were transformed with a cosmid containing the pur cluster, which determines the puromycin biosynthetic pathway from Streptomyces alboniger. bldA+ transformants produced puromycin in typical amounts, whereas bldA39 transformants did so at drastically decreased levels. Transformation of low producers with the wild-type bldA gene reverted this phenotype to normal production. These data, in addition to the presence of a TTA codon in the amino-terminal coding region of the pur10 and pur6 genes of the pur cluster, suggest that the puromycin biosynthetic pathway is translationally dependent on the bldA gene product, a tRNALeu.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tercero JA,Espinosa JC,Jiménez Adoi
10.1016/s0014-5793(97)01564-0subject
Has Abstractpub_date
1998-01-16 00:00:00pages
221-3issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(97)01564-0journal_volume
421pub_type
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