A D-antifreeze polypeptide displays the same activity as its natural L-enantiomer.

abstract：:A pumpkin 2S albumin with ribonuclease (RNase) activity was purified from pumpkin seeds (Cucurbita sp.) by liquid chromatographic techniques. It manifested potent RNase activity toward baker's yeast RNA and calf liver RNA, and some polyhomoribonucleotides, including poly(A), poly(U) and poly(C) but not poly(G). Moreov...

journal_title：FEBS letters

authors： Fang EF,Wong JH,Lin P,Ng TB

更新日期：2010-09-24 00:00:00

abstract：:Currently described substrates of the bacterial Tat protein transport system are directed for export by signal peptides containing a pair of invariant arginine residues. The signal peptide of the TtrB subunit of Salmonella enterica tetrathionate reductase contains a single arginine residue but is nevertheless able to ...

journal_title：FEBS letters

authors： Hinsley AP,Stanley NR,Palmer T,Berks BC

更新日期：2001-05-18 00:00:00

abstract：:We examined the effects of histidine residues that were artificially introduced into complementarity-determining regions of antibodies on antigen-antibody interactions at different pH values. Using a monoclonal antibody specific for hen egg-white lysozyme and three mutant antibodies that contained a histidine residue,...

journal_title：FEBS letters

authors： Ito W,Sakato N,Fujio H,Yutani K,Arata Y,Kurosawa Y

更新日期：1992-08-31 00:00:00

abstract：:Amyloid-beta peptide (Abeta), the major constituent of senile plaques in the Alzheimer's disease (AD) brain, is the main source of oxidative stress leading to neurodegeneration. The methionine residue in this peptide is reported to be responsible for neurotoxicity. Structurally similar substitution with methionine 35 ...

journal_title：FEBS letters

authors： Dai XL,Sun YX,Jiang ZF

更新日期：2007-04-03 00:00:00

abstract：:Sequence homology was found by computer analysis between potato spindle tuber viroid (PSTV) RNA and U3B snRNA of Novikoff hepatoma cells. This homology is colinear in arrangement, extends in length to 81% of the entire U3B snRNA molecule and is involved in the PSTV molecule unique sites which, if depicted in terms of ...

journal_title：FEBS letters

authors： Kiss T,Pósfai J,Solymosy F

更新日期：1983-11-14 00:00:00

abstract：:Peroxisome proliferator-activated receptor gamma (PPARγ), a ligand-regulated nuclear hormone receptor, plays critical roles in metabolism and adipogenesis. PPARγ ligands such as thiazolidinediones (TZDs) exert insulin sensitizing and anti-inflammatory effects primarily through action on adipocytes, and are thus widely...

journal_title：FEBS letters

authors： Sugii S,Evans RM

更新日期：2011-07-07 00:00:00

abstract：:A long-term goal of the field of interactome modeling is to understand how global and local properties of complex macromolecular networks impact on observable biological properties, and how changes in such properties can lead to human diseases. The information available at this stage of development of the field provid...

journal_title：FEBS letters

authors： Vidal M

更新日期：2005-03-21 00:00:00

abstract：:Ankyrin repeat domain 17 (Ankrd17) encodes an ubiquitously expressed protein with two clusters of ankyrin repeats. We have used gene targeting strategy to ablate the Ankrd17 gene in mouse. The Ankrd17-deficient mice died between embryonic day (E) 10.5 and E11.5 due to cardiovascular defects. Serious hemorrhages were d...

journal_title：FEBS letters

authors： Hou SC,Chan LW,Chou YC,Su CY,Chen X,Shih YL,Tsai PC,Shen CK,Yan YT

更新日期：2009-09-03 00:00:00

abstract：:The main role of voltage-gated proton channels (Hv1) is to extrude protons from the intracellular milieu when, mediated by different cellular processes, the H(+) concentration increases. Hv1 are exquisitely selective for protons and their structure is homologous to the voltage sensing domain (VSD) of other voltage-gat...

journal_title：FEBS letters

authors： Castillo K,Pupo A,Baez-Nieto D,Contreras GF,Morera FJ,Neely A,Latorre R,Gonzalez C

更新日期：2015-11-14 00:00:00

abstract：:CpG dinucleotides are efficiently methylated in vertebrate genomes except in the CpG islands having a high C+G content. Methylated CpGs are the single most mutated dinucleotide. Sequences surrounding disease causing CpG mutation sites were analyzed from locus-specific mutation databases. Both tetra- and heptanucleotid...

journal_title：FEBS letters

authors： Ollila J,Lappalainen I,Vihinen M

更新日期：1996-11-04 00:00:00

abstract：:Until cytoplasmic recapping was discovered, decapping was thought to irreversibly destine an mRNA to degradation. Contradicting this idea, we readily observe mRNAs targeted by cytoplasmic capping in uncapped, yet stable forms. 5' rapid amplification of cDNA ends (RACE) shows that nearly all uncapped ends correspond to...

journal_title：FEBS letters

authors： Berger MR,Alvarado R,Kiss DL

更新日期：2019-04-01 00:00:00

abstract：:The intracellular expression of recombinant antibodies in mammalian cells is an experimental strategy to inhibit in vivo the function of selected molecules. However, one limitation of this technology is represented by the unpredictable behaviour of antibodies, under conditions of intracellular expression. For this rea...

journal_title：FEBS letters

authors： Gargano N,Cattaneo A

更新日期：1997-09-15 00:00:00

abstract：:The conformation in solution of the biantennary complex type oligosaccharide unit derived from human serotransferrin has been investigated using 1H-1H Nuclear Overhauser Effect (NOE) measurements at 300 MHz. From quantitation of the NOE, the alpha(1-3) antenna is shown to exist in a preferred solution conformation wit...

journal_title：FEBS letters

authors： Homans SW,Dwek RA,Fernandes DL,Rademacher TW

更新日期：1982-12-27 00:00:00

abstract：:A novel calcium-dependent serine proteinase (CASP) secreted from malignant hamster embryo fibroblast Ni 12C2 degrades extracellular matrix proteins. A complementary DNA encoding CASP has been isolated with the use of oligonucleotide probes synthesized based on partial amino acid sequences of CASP. The complete amino a...

journal_title：FEBS letters

authors： Kinoshita H,Sakiyama H,Tokunaga K,Imajoh-Ohmi S,Hamada Y,Isono K,Sakiyama S

更新日期：1989-07-03 00:00:00

abstract：:The amino-terminal part of prion protein (PrP), containing a series of octapeptide repeats with the consensus sequence PHGGGWGQ, has been implicated in the binding of copper ion. This region possesses amino acid residues susceptible to oxidation, such as histidine, lysine, arginine and proline. In this study, we have ...

journal_title：FEBS letters

authors： Shiraishi N,Nishikimi M

更新日期：2002-01-30 00:00:00

abstract：:The cyclic, imido acid containing pentapeptides cyclo(Asp-Trp-(NMe)Nle-Asp-Phe) (cpp[NMeNle(3)]) and cyclo(Asp-Trp-Pro-Asp-Phe) (cpp[Pro(3)]) have been investigated by 1H-NMR spectroscopy in DMSO and by restrained molecular dynamics methods. The spectra indicate the existence of at least four cis/trans isomers for cpp...

journal_title：FEBS letters

authors： Weisshoff H,Wieprecht T,Henklein P,Frömmel C,Antz C,Mügge C

更新日期：1996-06-03 00:00:00

abstract：:Prohormone convertases (PCs) 1 and 2 are thought to mediate the proteolytic cleavage of many peptide precursors. Endogenous inhibitors of both PC1 and PC2 have now been identified; the 7B2 protein is a nanomolar inhibitor of PC2, while the novel protein proSAAS was recently reported to be a micromolar inhibitor of PC1...

journal_title：FEBS letters

authors： Cameron A,Fortenberry Y,Lindberg I

更新日期：2000-05-12 00:00:00

abstract：:The mitochondrial outer membrane contains a protein complex with at least eight subunits responsible for recognition and translocation of preproteins synthesized in the cytosol. Two subunits, the receptors Tom20 and Tom70, contain tetratrico peptide repeats that are thought to be involved in protein-protein interactio...

journal_title：FEBS letters

authors： Bömer U,Pfanner N,Dietmeier K

更新日期：1996-03-11 00:00:00

abstract：:Foot-and-mouth disease virus leader proteinase (L(pro)), a papain-like cysteine proteinase, has six acidic amino acids between 4 A and 11 A of the catalytic dyad of Cys51 and His148. In contrast, in papain and related enzymes, only one acidic residue lies within this distance. We have examined by site-directed mutagen...

journal_title：FEBS letters

authors： Kronovetr J,Skern T

更新日期：2002-09-25 00:00:00

abstract：:We report isolation of a murine gene, NPYR-D, which predicts an intronless novel G protein-coupled receptor of 375 amino acids. Percent identities of NPYR-D to the clone Y1, Y2, rat Y4/PP1 and human Y4/PP1 receptors are 45, 32, 92 and 76, respectively. Southern blots indicate that NPYR-D and human Y4/PP1 receptor gene...

journal_title：FEBS letters

authors： Gregor P,Millham ML,Feng Y,DeCarr LB,McCaleb ML,Cornfield LJ

更新日期：1996-02-26 00:00:00

abstract：:Treatment of rat thymus cell suspensions with dexamethasone resulted in inhibition of engaged RNA polymerase A, without significant change in free pool activity. Studies with the re-initiation inhibitor, rifamycin AF/0-13, and measurements of numbers of RNA polymerase A molecules and of elongation rates showed that th...

journal_title：FEBS letters

authors： Dembinski TC

更新日期：1984-07-23 00:00:00

abstract：:Raman spectra of active Na+,K+-ATPase from pig kidney and membrane-bound products of its two-stage trypsinolysis, including alpha-subunit hydrophobic regions as well as the intact beta-subunit and hydrophobic regions of alpha- and beta-subunits, were measured to calculate the secondary structure of hydrophilic and hyd...

journal_title：FEBS letters

authors： Ovchinnikov YuA,Arystarkhova EA,Arzamazova NM,Dzhandzhugazyan KN,Efremov RG,Nabiev IR,Modyanov NN

更新日期：1988-01-25 00:00:00

abstract：:An improved method for expressing and purifying bovine pancreatic ribonuclease from a synthetic gene using the lambda promoter controlled by a temperature-sensitive repressor is described. The procedure involves isolation in the presence of a refolding buffer containing oxidized and reduced glutathione, under conditio...

journal_title：FEBS letters

authors： McGeehan GM,Benner SA

更新日期：1989-04-10 00:00:00

abstract：:Pseudomonas aeruginosa exotoxin A (ETA) must be proteolytically nicked by furin at Arg279 before being translocated into the cytosol of target cells. A similar cleavage can also be obtained with trypsin. Using this assay we could show that the interaction with lipid bilayers can strongly influence the extent of nickin...

journal_title：FEBS letters

authors： Nordera P,Menestrina G

更新日期：1998-01-16 00:00:00

abstract：:Rats bearing the fast-growing AH-130 Yoshida ascites hepatoma showed a marked cachectic response which has been previously reported [Tessitore et al. (1987) Biochem. J. 241, 153-159]. Thus tumour-bearing animals showed significant decreases in body and muscle weight (soleus and gastrocnemius) as compared to both pair-...

journal_title：FEBS letters

authors： Llovera M,García-Martínez C,Agell N,Marzábal M,López-Soriano FJ,Argilés JM

更新日期：1994-02-07 00:00:00

abstract：:In the phylogeny of plant polyamine oxidases (PAOs), clade III members from angiosperms, such as Arabidopsis thaliana PAO5 and Oryza sativa PAO1, prefer spermine and thermospermine as substrates and back-convert both of these substrates to spermidine in vitro. A clade III representative of lycophytes, SelPAO5 from Sel...

journal_title：FEBS letters

authors： Sagor GH,Inoue M,Kim DW,Kojima S,Niitsu M,Berberich T,Kusano T

更新日期：2015-10-07 00:00:00

abstract：:We previously isolated, by differential display and 5' RACE (rapid amplification of cDNA ends), cDNAs corresponding to genes activated following cryptogein treatment of tobacco cell suspensions, among them tcI 7 (tcI for tobacco cryptogein Induced), a gene encoding a beta-subunit of proteasome. Here, we report that tc...

journal_title：FEBS letters

authors： Etienne P,Petitot AS,Houot V,Blein JP,Suty L

更新日期：2000-01-28 00:00:00

abstract：:Helicobacter pylori secretes a unique virulence factor, Tipalpha, that enters gastric cells and both stimulates the production of the TNF-alpha and activates the NF-kappaB pathway. The structures of a truncated version of Tipalpha (TipalphaN34) in two crystal forms are presented here. Tipalpha adopts a novel beta(1)al...

journal_title：FEBS letters

authors： Tosi T,Cioci G,Jouravleva K,Dian C,Terradot L

更新日期：2009-05-19 00:00:00

abstract：:The tuf gene of eubacteria, encoding the EF-tu elongation factor, was duplicated early in the evolution of the taxon. Phylogenetic and genomic location analysis of 20 complete eubacterial genomes suggests that this ancient duplication has been differentially lost and maintained in eubacteria. ...

journal_title：FEBS letters

authors： Lathe WC 3rd,Bork P

更新日期：2001-08-03 00:00:00

abstract：:Monomeric bovine pancreatic RNase A has been transformed into a dimeric ribonuclease with antitumor activity (Di Donato, A., Cafaro, V. and D'Alessio, G. (1994) J. Biol. Chem. 269, 17394-17396). This was accomplished by replacing the residues located in the RNase chain at positions 19, 28, 31, and 32, with proline, le...

journal_title：FEBS letters

authors： Cafaro V,Bracale A,Di Maro A,Sorrentino S,D'Alessio G,Di Donato A

更新日期：1998-10-16 00:00:00