mRNA 5' ends targeted by cytoplasmic recapping cluster at CAGE tags and select transcripts are alternatively spliced.

abstract：:Respiratory supercomplex factor (Rcf) 1 is a membrane-bound protein that modulates the activity of cytochrome c oxidase (CytcO) in Saccharomyces cerevisiae mitochondria. To investigate this regulatory mechanism, we studied the interactions of CytcO with potassium cyanide (KCN) upon removal of Rcf1. While the addition ...

journal_title：FEBS letters

authors： Rydström Lundin C,Brzezinski P

更新日期：2017-12-01 00:00:00

abstract：:Perhydrohistrionicotoxin at micromolar concentrations blocked the nicotine-evoked transmitter release from perfused striatal (dopaminergic) and hippocampal (cholinergic) nerve terminals. Perhydrohistrionicotoxin failed to compete with [3H]nicotine for its high-affinity binding site in rat brain, suggesting that the ac...

journal_title：FEBS letters

authors： Rapier C,Wonnacott S,Lunt GG,Albuquerque EX

更新日期：1987-02-23 00:00:00

abstract：:The multiline signal of the S(2) state in Photosystem II was measured both in frozen-solution and single-crystal preparations from the cyanobacterium Thermosynechococcus elongatus. The frozen-solution EPR spectrum shows a gaussian-like line shape without any resolution of Mn hyperfine couplings. This line shape can be...

journal_title：FEBS letters

authors： Teutloff C,Kessen S,Kern J,Zouni A,Bittl R

更新日期：2006-06-26 00:00:00

abstract：:VASP (vasodilator-stimulated phosphoprotein), a protein associated with microfilaments at cellular contact sites, has been identified as a ligand for profilin and zyxin, two proteins also involved in microfilament dynamics and organization at these regions. Here, we report that VASP also directly binds to vinculin, an...

journal_title：FEBS letters

authors： Reinhard M,Rüdiger M,Jockusch BM,Walter U

更新日期：1996-12-09 00:00:00

abstract：:Since the discovery of RNAi, its mechanism in plants and animals has been intensively studied, widely exploited as a research tool, and used for a number of potential commercial applications. In this article, we discuss the platforms for delivering RNAi in plants. We provide a brief background to these platforms and c...

journal_title：FEBS letters

authors： Watson JM,Fusaro AF,Wang M,Waterhouse PM

更新日期：2005-10-31 00:00:00

abstract：:It is known that narcotic antagonists interact with many cholinergic pathways but less in known about specific mechanisms. Using neonatal rat myoballs to study single channel behaviour of the acetylcholinegated nicotinic receptor, it was found that micromolar concentrations of naltrexone had no effect on channel condu...

journal_title：FEBS letters

authors： Madsen BW,Albuquerque EX

更新日期：1985-03-11 00:00:00

abstract：:Amyloid-beta peptide (Abeta), the major constituent of senile plaques in the Alzheimer's disease (AD) brain, is the main source of oxidative stress leading to neurodegeneration. The methionine residue in this peptide is reported to be responsible for neurotoxicity. Structurally similar substitution with methionine 35 ...

journal_title：FEBS letters

authors： Dai XL,Sun YX,Jiang ZF

更新日期：2007-04-03 00:00:00

abstract：:Treatment of either crude or purified preparations of the gamma-aminobutyrate (GABA)/benzodiazepine receptor complex with arginine-specific reagents resulted in a time- and concentration-dependent loss of [3H]muscimol binding activity. Following exposure to either 2,3-butanedione or phenylglyoxal (less than or equal t...

journal_title：FEBS letters

authors： Widdows KB,Kirkness EF,Turner AJ

更新日期：1987-09-28 00:00:00

abstract：:PsbU is one of the extrinsic proteins in red algal Photosystem II (PSII) and functions to optimize the availability of Ca(2+) and Cl(-) cofactors for water oxidation. To determine the functional residue of PsbU, we constructed various PsbU mutants from a red alga Cyanidium caldarium and reconstituted these mutants wit...

journal_title：FEBS letters

authors： Okumura A,Sano M,Suzuki T,Tanaka H,Nagao R,Nakazato K,Iwai M,Adachi H,Shen JR,Enami I

更新日期：2007-11-13 00:00:00

abstract：:We have generated a mouse model for hepatic ischemia in which surgical subcutaneous transposition of the spleen allows hepatic ischemia to be applied without affecting other tissues. Using this mouse model we investigated the relationship between the length of ischemic periods in the liver and subsequent liver functio...

journal_title：FEBS letters

authors： Onishi I,Tani T,Hashimoto T,Shimizu K,Yagi M,Yamamoto K,Yoshioka K

更新日期：1997-12-29 00:00:00

abstract：:Highly selective affinity labeling of the primer site of E. coli DNA polymerase I was performed with the 5'-reactive derivatives of oligothymidylate in the presence of poly(dA) template. Subtilysine cleavage proved that the site of affinity modification belonged to the 'Klenow' part of DNA polymerase I. If taken separ...

journal_title：FEBS letters

authors： Mitina RL,Mustaev AA,Zaychikov EF,Khomov VV,Lavrik OI

更新日期：1990-10-15 00:00:00

abstract：:The cyclic, imido acid containing pentapeptides cyclo(Asp-Trp-(NMe)Nle-Asp-Phe) (cpp[NMeNle(3)]) and cyclo(Asp-Trp-Pro-Asp-Phe) (cpp[Pro(3)]) have been investigated by 1H-NMR spectroscopy in DMSO and by restrained molecular dynamics methods. The spectra indicate the existence of at least four cis/trans isomers for cpp...

journal_title：FEBS letters

authors： Weisshoff H,Wieprecht T,Henklein P,Frömmel C,Antz C,Mügge C

更新日期：1996-06-03 00:00:00

abstract：:Catenins are peripheral cytoplasmic proteins originally identified in association with the mouse epithelial cell adhesion molecule E-cadherin. Molecular cloning and primary structure analysis demonstrated that alpha-catenin is homologous to vinculin and the beta-catenin is homologous to human plakoglobin and the Droso...

journal_title：FEBS letters

authors： Butz S,Kemler R

更新日期：1994-11-28 00:00:00

abstract：:As a result of Myc-dependent transcription of the LDH-A gene, Myc-transformed cells (Rat1-Myc) exhibit increased lactate production rates (LPR) even under aerobic conditions (the Warburg effect). Recently, the increased susceptibility to stress-induced apoptosis associated with Myc transfection has been linked to the ...

journal_title：FEBS letters

authors： Papas KK,Sun L,Roos ES,Gounarides JS,Shapiro M,Nalin CM

更新日期：1999-03-12 00:00:00

abstract：:We have identified a cDNA encoding a 212 amino acid protein (Nm23-H5) with 27-31% identity to the human members of the nm23/nucleoside diphosphate (NDP) kinase gene family. The nm23-H5 gene is located on chromosome 5q23-31 and is transcribed as one main transcript of 1.1 kb which is highly and specifically expressed i...

journal_title：FEBS letters

authors： Munier A,Feral C,Milon L,Pinon VP,Gyapay G,Capeau J,Guellaen G,Lacombe ML

更新日期：1998-09-04 00:00:00

abstract：:Upon dimerization by oxidation, Hsp33 functions as a molecular chaperone in prokaryotes. Previously published structures of both the inactive and active species are of doubtful relevance to the solution conformations since the inactive (reduced) crystal structure was dimeric, while the active (oxidized) species was cr...

journal_title：FEBS letters

authors： Lee YS,Ryu KS,Kim SJ,Ko HS,Sim DW,Jeon YH,Kim EH,Choi WS,Won HS

更新日期：2012-02-17 00:00:00

abstract：:The gene for titin, a 4MDa myofibrillar protein, was analysed in golden hamster DNAs from different sources, using human cDNA probes and PCR. In the DNA from the BHK-21-Bi subline of baby hamster kidney cells, extended sequences coding for Z-line associated domains were missing, indicating a deletion that renders titi...

journal_title：FEBS letters

authors： Jäckel M,Witt C,Antonova O,Curdt I,Labeit S,Jockusch H

更新日期：1997-05-12 00:00:00

abstract：:The photosynthetic reaction center is one of the most complicated molecular complexes. Transducing photon energy to a transmembrane electrochemical potential difference for protons, it is the direct or indirect energy source for virtually all life. We show here that it operates in a simple, battery-like manner, with a...

journal_title：FEBS letters

authors： van Rotterdam BJ,Crielaard W,van Stokkum IH,Hellingwerf KJ,Westerhoff HV

更新日期：2002-01-02 00:00:00

abstract：:Physiological and pathological states that are associated with elevated plasma fatty acids (FAs) increase uncoupling protein 2 (UCP2) mRNA in white adipose tissue and UCP3 mRNA in skeletal muscle and heart. A direct effect of unsaturated fatty acids from all classes has been shown in various cultured cells. There is e...

journal_title：FEBS letters

authors： Thompson MP,Kim D

更新日期：2004-06-18 00:00:00

abstract：:Lateral mobility of the vasopressin renal-type V2-receptor was investigated in LLC-PK1 porcine epithelial cells using the technique of fluorescence microphotolysis (photobleaching) and a rhodamine-labelled vasopressin analogue. At various times after ligand addition, cells were analyzed for both receptor lateral mobil...

journal_title：FEBS letters

authors： Jans DA,Peters R,Fahrenholz F

更新日期：1990-11-12 00:00:00

abstract：:The role of tRNA in translating the genetic code has received considerable attention over the last 50 years, and we now know in great detail how particular amino acids are specifically selected and brought to the ribosome in response to the corresponding mRNA codon. Over the same period, it has also become increasingl...

journal_title：FEBS letters

authors： Banerjee R,Chen S,Dare K,Gilreath M,Praetorius-Ibba M,Raina M,Reynolds NM,Rogers T,Roy H,Yadavalli SS,Ibba M

更新日期：2010-01-21 00:00:00

abstract：:The assembly of cytochrome c oxidase subunits I-III was studied in vitro in isolated rat liver mitochondria pre-labeled with [35S]methionine. Individual subunits were immunoabsorbed with monospecific antibodies. Isolated heme a from rat liver mitochondria, when added to radiolabeled mitochondria, induced assembly of s...

journal_title：FEBS letters

authors： Wielburski A,Nelson BD

更新日期：1984-11-19 00:00:00

abstract：:Chlorogenic acid (CGA; 3-o-caffeoylquinic acid), a phenylpropanoid metabolite of plants, was oxidized by H2O2 in the presence of horseradish peroxidase. The primary and secondary oxidized products both were free radicals which gave EPR multiline signals at g = 2.0044 and 2.0042 in the presence of zinc as a spin stabil...

journal_title：FEBS letters

authors： Yamasaki H,Grace SC

更新日期：1998-02-06 00:00:00

abstract：:Posttranslational modifications of circadian oscillator components are crucial for the generation of circadian rhythms. Among those phosphorylation plays key roles ranging from regulating degradation, complex formation, subcellular localization and activity. Although most of the known clock proteins are phosphoprotein...

journal_title：FEBS letters

authors： Reischl S,Kramer A

更新日期：2011-05-20 00:00:00

abstract：:Cells of Saccharomyces cerevisiae were loaded with indo-1, by incubation in a medium of pH 4.5, which contained penta-potassium indo-1. Cells were then washed and resuspended in a buffer of pH 4.0. The emission fluorescence spectra were recorded between 390 and 500 nm (excitation at 355 nm) and the autofluorescent spe...

journal_title：FEBS letters

authors： Halachmi D,Eilam Y

更新日期：1989-10-09 00:00:00

abstract：:The bacterial toxin VacA produced by H. pylori induces large vacuoles in several types of cultured cells such as HeLa cells or gastric cells. To determine the mechanism of vacuolation induced by this toxin we employed several inhibitors of membrane trafficking and endocytosis. The development of vacuolation induced by...

journal_title：FEBS letters

authors： Seto K,Hayashi-Kuwabara Y,Yoneta T,Suda H,Tamaki H

更新日期：1998-07-24 00:00:00

abstract：:Decarboxylation of pyruvate by pyruvate decarboxylase (EC 4.1.1.1) was performed in a reaction mixture containing 50% deuterium. The isolated product, acetaldehyde, was investigated directly by 1H NMR and by mass spectrometry after conversion to the 2,4-dinitrophenyl hydrazone. The protium content of 56% at acetaldehy...

journal_title：FEBS letters

authors： Ermer J,Schellenberger A,Hübner G

更新日期：1992-03-09 00:00:00

abstract：:Recent advances in our understanding of the structure of highly amplified DNA sequences in Streptomyces fradiae and lividans have enabled us to formulate a possible mechanism by which amplification may occur. An essential feature of the model is the generation of an amplification precursor, which comprises a circulari...

journal_title：FEBS letters

authors： Young M,Cullum J

更新日期：1987-02-09 00:00:00

abstract：:The cDNA coding for a novel member of the cysteine-rich protein family was isolated from a rat brain cDNA library. It encodes a protein, denoted cysteine-rich protein 2 (CRP2), of 208 amino acid residues containing two tandem repeats of an unusual LIM/double zinc-finger-like motif. The ubiquitous tissue distribution a...

journal_title：FEBS letters

authors： Okano I,Yamamoto T,Kaji A,Kimura T,Mizuno K,Nakamura T

更新日期：1993-10-25 00:00:00

abstract：:Human natural killer (NK) cells possess an arsenal of receptors programmed to regulate the NK cell functions, once encountering a target cell. In general, the activating receptors mediate cytotoxicity when engaged by their tumor specific, stress induced, virally encoded, or rarely, self ligands. Whereas, the inhibitor...

journal_title：FEBS letters

authors： Stanietsky N,Mandelboim O

更新日期：2010-12-15 00:00:00