Kinases and phosphatases in the mammalian circadian clock.

abstract：:It has previously been shown that the levels of poly(ADP-ribose)polymerase and polymers of ADP-ribose that co-purify with the nuclear matrix in regenerating liver fluctuate with the levels of in vivo DNA replication [(1988) FEBS Lett. 236, 362-366]. We have now electrophoretically identified lamins A and C, and poly(A...

journal_title：FEBS letters

authors： Pedraza-Reyes M,Alvarez-Gonzalez R

更新日期：1990-12-17 00:00:00

abstract：:Gastrin (G17) and N-carboxymethylgastrin (G17-Gly) have been shown to stimulate the growth of colon cancer cells both in vivo and in vitro. The identity of the receptor mediating these effects is controversial. A recent study demonstrated the presence of a low affinity binding site for G17 and G17-Gly on the DLD-1 hum...

journal_title：FEBS letters

authors： Ahmed S,Budai B,Herédi-Szabó K,Farkas J,Tóth G,Murphy RF,Lovas S

更新日期：2004-01-02 00:00:00

abstract：:The proteolysis of beta-amyloid (Abeta) requires neprylisin, an enzyme that has been shown as reduced in Alzheimer's disease (AD). We investigated whether a decrease in neprilysin levels contributes to the accumulation of amyloid deposits not only in AD but also in the normal aging. We analyzed neprilysin mRNA and pro...

journal_title：FEBS letters

authors： Russo R,Borghi R,Markesbery W,Tabaton M,Piccini A

更新日期：2005-11-07 00:00:00

abstract：:F-box/LRR-repeat protein 2 (FBXL2), a component of Skp-Cullin-F box (SCF) ubiquitin E3 ligase, has been shown to inhibit tumorigenesis by targeting and ubiquitinating several oncoproteins. However, its role in gastric cancer remains poorly understood. Here, by tandem mass spectrometry, we show that FBXL2 interacts wit...

journal_title：FEBS letters

authors： Li LQ,Pan D,Chen H,Zhang L,Xie WJ

更新日期：2016-02-01 00:00:00

abstract：:Rat liver fructose 1,6-bisphosphatase can be protected against partial inactivation by N-ethylmaleimide by low concentrations of fructose 2,6-bisphosphate or high concentrations of fructose 1,6-bisphosphate. The partially inactivated enzyme has a much reduced sensitivity to high substrate inhibition and has lost the s...

journal_title：FEBS letters

authors： Meek DW,Nimmo HG

更新日期：1983-08-22 00:00:00

abstract：:To determine the amino acid residues required for the signal-transducing activity of the human c-Ha-Ras protein, we introduced point mutations at residues 45-54 near the 'effector region' (residues 32-40). We transfected PC12 cells with these mutant genes and also micro-injected the mutant proteins, bound with an unhy...

journal_title：FEBS letters

authors： Fujita-Yoshigaki J,Shirouzu M,Koide H,Nishimura S,Yokoyama S

更新日期：1991-12-09 00:00:00

abstract：:Physiological amyloid aggregation occurs within the nuclei of stress-treated cells. These structures, termed Amyloid bodies (A-bodies), assemble through the rapid accumulation of proteins into dense membrane-less organelles, which possess the same biophysical properties as plaques observed in many amyloid-based diseas...

journal_title：FEBS letters

authors： Marijan D,Tse R,Elliott K,Chandhok S,Luo M,Lacroix E,Audas TE

更新日期：2019-11-01 00:00:00

abstract：:Bilayers made of dioleoylphosphatidylcholine (DOPC)/dipalmitoylphosphatidylcholine (DPPC) mixture containing or not cholesterol (Chl) were used to investigate the interaction of a carrier peptide with membranes. Atomic force microscopy revealed that the C-terminal 9-32 fragment of human calcitonin (hCT (9-32)), free o...

journal_title：FEBS letters

authors： Boichot S,Krauss U,Plénat T,Rennert R,Milhiet PE,Beck-Sickinger A,Le Grimellec C

更新日期：2004-07-02 00:00:00

abstract：:ADP-ribosylation of HeLa nonhistone proteins was investigated by using [3H]adenosine as an in vivo radioactive label. The aim was to determine basic differences in the patterns of modification of interphase and metaphase nonhistones. Fluorography revealed a relatively small number of modified proteins for isolated met...

journal_title：FEBS letters

authors： Adolph KW,Song MK

更新日期：1985-03-11 00:00:00

abstract：:Plant receptor-like kinases (RLKs) share their evolutionary origin with animal interleukin-1 receptor-associated kinase (IRAK)/Pelle family of soluble kinases and are distinguished by having tyrosine as 'gatekeeper'. This position is adjacent to the hinge region and is hidden in a hydrophobic pocket of the catalytic c...

journal_title：FEBS letters

authors： Samaddar S,Dutta A,Sinharoy S,Paul A,Bhattacharya A,Saha S,Chien KY,Goshe MB,DasGupta M

更新日期：2013-09-17 00:00:00

abstract：:Recent studies have revealed the presence of an atypical progesterone receptor on the plasma membrane of a relatively small subpopulation of human sperm cells. In this study, we show that the receptor is present in many cells in a latent form and that the receptor function in these cells is switched on asynchronously ...

journal_title：FEBS letters

authors： Mendoza C,Tesarik J

更新日期：1993-09-06 00:00:00

abstract：:We have isolated a cDNA encoding a precursor of dermorphin from the skin of Pachymedusa dacnicolor. Besides four copies of this opioid peptide, the deduced sequence also contains the genetic information for a novel peptide Tyr-Ile-Phe-His-Leu-Met-Asp-NH2. This differs from Met-deltorphin by the presence of Ile at posi...

journal_title：FEBS letters

authors： Wechselberger C,Severini C,Kreil G,Negri L

更新日期：1998-06-05 00:00:00

abstract：:Luminescence of Tb3+ ions bound to a calmodulin fragment has been studied. It is shown that during their lifetime excited ions dissociate from the peptide. If concentration of free peptide is high enough they can be coordinated again. As a consequence, observed terbium luminescence lifetime and intensity depends not o...

journal_title：FEBS letters

authors： Dadlez M,Góral J,Bierzyński A

更新日期：1991-04-22 00:00:00

abstract：:Hydrogen/deuterium (H/D) exchange measurements in low and moderate concentrations of GuHCI were conducted on the side chain H(N) atoms of the seven tryptophans of pseudo wild-type human carbonic anhydrase II. Tryptophans 5, 16 and 245, situated in or close to the N-terminal domain were found to have little protection ...

journal_title：FEBS letters

authors： Jonasson P,Kjellsson A,Sethson I,Jonsson BH

更新日期：1999-02-26 00:00:00

abstract：:Regulatory T-cells (Tregs) are the guardians of peripheral tolerance acting to prevent autoimmune diseases such as systemic lupus erythomatosus (SLE) and rheumatoid arthritis (RA). Defects in Tregs have been reported in these two diseases despite significant differences in their clinical phenotype and pathogenesis. In...

journal_title：FEBS letters

authors： Chavele KM,Ehrenstein MR

更新日期：2011-12-01 00:00:00

abstract：:We have analyzed the specificity of the actinomycin D-DNA interaction. The 'footprint' method has been used in this investigation. It is shown that: (i) The presence of dinucleotide GC or GG is required for binding of a single drug molecule. (ii) The strong binding sites are encoded by tetranucleotide XGCY; where X no...

journal_title：FEBS letters

authors： Scamrov AV,Beabealashvilli RS

更新日期：1983-11-28 00:00:00

abstract：:Human monoclonal antibodies often display limited thermodynamic and colloidal stabilities. This behavior hinders their production, and places limitations on the development of novel formulation conditions and therapeutic applications. Antibodies are highly diverse molecules, with much of the sequence variation observe...

journal_title：FEBS letters

authors： Rouet R,Lowe D,Christ D

更新日期：2014-01-21 00:00:00

abstract：:Fourier transform infrared spectroscopy has been used to study the solution structure and thermal stability of the extracellular fragment of human transferrin receptor (tfRt) at extracellular and endosomal pH. At extracellular pH tfRt is composed of 56% alpha-helix, 19% beta-sheet and 14% turns. Upon acidification to ...

journal_title：FEBS letters

authors： Hadden JM,Bloemendal M,Haris PI,van Stokkum IH,Chapman D,Srai SK

更新日期：1994-08-22 00:00:00

abstract：:We have previously reported that Monad, a novel WD40 repeat protein, potentiates apoptosis induced by tumor necrosis factor-alpha(TNF-alpha) and cycloheximide (CHX). By affinity purification and mass spectrometry, we identified RNA polymerase II-associated protein 3 (RPAP3) as a binding protein of Monad. Overexpressio...

journal_title：FEBS letters

authors： Itsuki Y,Saeki M,Nakahara H,Egusa H,Irie Y,Terao Y,Kawabata S,Yatani H,Kamisaki Y

更新日期：2008-07-09 00:00:00

abstract：:The amino acid sequence of the alpha-subunit of Gi, the human adenylate cyclase inhibiting GTP-binding protein, has been deduced from the nucleotide sequence of a DNA clone complementary to Gi alpha mRNA from differentiated U937 cells. The cDNA encodes a polypeptide of 355 amino acids (Mr 40456). The amino acid sequen...

journal_title：FEBS letters

authors： Didsbury JR,Ho YS,Snyderman R

更新日期：1987-01-26 00:00:00

abstract：:A Ca2+ indicator arsenazo III was used to demonstrate calcium uptake activity of symbiosomes and the peribacteroid membrane (PBM) vesicles isolated from broad bean root nodules and placed in the medium containing ATP and Mg2+ ions. This process was shown to be rapidly stopped by vanadate, completely reversed in the pr...

journal_title：FEBS letters

authors： Andreev IM,Dubrovo PN,Krylova VV,Izmailov SF

更新日期：1999-03-19 00:00:00

abstract：:We have studied functional properties of peptide transport in the pheochromocytoma neuroendocrine cell line from rat. The neutral peptide D-Phe-L-Ala (resistant to hydrolysis) is a good substrate for uptake into these cells. Transport is substantially inhibited by diethylpyrocarbonate pretreatment and is stimulated by...

journal_title：FEBS letters

authors： Hussain I,Zanic-Grubisic T,Kudo Y,Boyd CA

更新日期：2001-11-23 00:00:00

abstract：:NG-Methyl-L-arginine (L-NMA) and NG-nitro-L-arginine (L-NNA) inhibited NO-induced cGMP accumulation in porcine aortic endothelial cells with half-maximally effective concentrations of 15 and 3.4 microM, respectively. The effects of both compounds were reversible, but the L-NNA-induced inhibition was only reversed by w...

journal_title：FEBS letters

authors： Mayer B,Schmid M,Klatt P,Schmidt K

更新日期：1993-10-25 00:00:00

abstract：:The [Cu(I)-Cu(II)] half-met form of the dinuclear copper site of tyrosinase has been probed by continuous wave electron paramagnetic resonance (EPR) and hyperfine sublevel correlation (HYSCORE) spectroscopy in the presence and absence of inhibitors. In all cases the EPR spectrum is indicative of a d(x(2)-y(2)) ground ...

journal_title：FEBS letters

authors： van Gastel M,Bubacco L,Groenen EJ,Vijgenboom E,Canters GW

更新日期：2000-06-02 00:00:00

abstract：:Activation-induced cytidine deaminase (AID), a member of the APOBEC family that induces antibody diversification, has been shown to inhibit the replication of hepatitis B virus, Kaposi's sarcoma-associated herpesvirus, and retro-transposons. However, whether AID can inhibit human immunodeficiency virus 1 (HIV-1) repli...

journal_title：FEBS letters

authors： Wang R,Zhang X,Ding H,Qiao Y,Han X,Geng W,Guan G,Cui H,Zhao B,Wu Y,Liang G,Shang H

更新日期：2018-01-01 00:00:00

abstract：:Neuronal activity is often associated with changes in gene expression. By a two-dimensional cDNA-display system, restriction landmark cDNA scanning, we identified a novel gene whose expression in the hippocampus was up-regulated by kainate stimulation. The mRNA expression was detected only in brain and up-regulated by...

journal_title：FEBS letters

authors： Nakayama T,Yaoi T,Yasui M,Kuwajima G

更新日期：1998-05-22 00:00:00

abstract：:Treatment of U937 cells with dolichyl phosphate led to an increase in the activity of the ICE family protease CPP32, accompanied with cleavage of pre-CPP32 to generate p17. Peptide inhibitors YVAD-cmk and Z-Asp-CH2-DCB (specific to ICE) and DEVD-CHO (specific to CPP32) blocked the dolichyl phosphate-induced apoptosis....

journal_title：FEBS letters

authors： Yokoyama Y,Okubo T,Ozawa S,Nagai F,Ushiyama K,Kano I,Shioda M,Kubo H,Takemura M,Namiki H,Yasugi E,Oshima M,Seyama Y,Kano K

更新日期：1997-07-21 00:00:00

abstract：:Recombinant adipogenesis inhibitory factor (AGIF) was purified to homogeneity from the conditioned medium of COS-1 cells transfected with human AGIF cDNA. The amino-terminal sequence analysis of the mature AGIF revealed that AGIF was produced as a precursor consisting of 199 amino acids and processed into a mature for...

journal_title：FEBS letters

authors： Ohsumi J,Miyadai K,Kawashima I,Ishikawa-Ohsumi H,Sakakibara S,Mita-Honjo K,Takiguchi Y

更新日期：1991-08-19 00:00:00

abstract：:The existence of the internal promoter Pi in the 16 S/23 S intergenic spacers of the rRNA operons of an eubacterium Escherichia coli and archaebacterium Halobacterium halobium is proposed. The possible functional significance of these promoters is discussed. ...

journal_title：FEBS letters

authors： Mankin AS,Skripkin EA,Kagramanova VK

更新日期：1987-07-27 00:00:00

abstract：:The R2 protein component of mouse ribonucleotide reductase has been obtained from overproducing Escherichia coli bacteria. It has been crystallized using NaCl as precipitant. The crystals are orthorhombic, space group C222(1), with cell dimensions a = 76.9 A, b = 108.9 A, c = 92.7 A and diffract to at least 2.5 A. The...

journal_title：FEBS letters

authors： Nielsen BB,Kauppi B,Thelander M,Thelander L,Larsen IK,Eklund H

更新日期：1995-10-16 00:00:00