Structure and thermal stability of the extracellular fragment of human transferrin receptor at extracellular and endosomal pH.


:Fourier transform infrared spectroscopy has been used to study the solution structure and thermal stability of the extracellular fragment of human transferrin receptor (tfRt) at extracellular and endosomal pH. At extracellular pH tfRt is composed of 56% alpha-helix, 19% beta-sheet and 14% turns. Upon acidification to endosomal pH the alpha-helical content of the protein is reduced and the beta-sheet content increased by nearly 10%. At extracellular pH, the midpoint temperature of thermal denaturation (Tm) for the loss of secondary and tertiary structure, and the formation of aggregated structures, is 71 degrees C. At endosomal pH this temperature is reduced by approximately 15 degrees C. The apparent entropies of thermal denaturation indicate that the native structure of tfRt at endosomal pH is far more flexible than at extracellular pH.




FEBS letters


Hadden JM,Bloemendal M,Haris PI,van Stokkum IH,Chapman D,Srai SK




Has Abstract


1994-08-22 00:00:00














  • Cellular strategies for proteolytic targeting during migration and invasion.

    abstract::Cell migration over or through the extracellular matrix (ECM) is an integral feature of both physiological and pathological processes. Regulation of the changing cell-ECM interactions involved can be effected by proteolysis and requires strict spatial and temporal targeting of proteinase activity. The versatile use of...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Ellis V,Murphy G

    更新日期:2001-09-28 00:00:00

  • A new opioid peptide predicted from cloned cDNAs from skin of Pachymedusa dacnicolor and Agalychnis annae.

    abstract::We have isolated a cDNA encoding a precursor of dermorphin from the skin of Pachymedusa dacnicolor. Besides four copies of this opioid peptide, the deduced sequence also contains the genetic information for a novel peptide Tyr-Ile-Phe-His-Leu-Met-Asp-NH2. This differs from Met-deltorphin by the presence of Ile at posi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wechselberger C,Severini C,Kreil G,Negri L

    更新日期:1998-06-05 00:00:00

  • Regulation of c-fos gene transcription and myeloid cell differentiation by acute myeloid leukemia 1 and acute myeloid leukemia-MTG8, a chimeric leukemogenic derivative of acute myeloid leukemia 1.

    abstract::Both acute myeloid leukemia 1 and c-Fos are regulatory factors of hematopoietic cell differentiation. We identified that the c-fos promoter contains an acute myeloid leukemia 1 binding site at nucleotide positions -6-+14. c-fos promoter activity was induced by transient overexpression of acute myeloid leukemia 1 in Ju...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hwang ES,Hong JH,Bae SC,Ito Y,Lee SK

    更新日期:1999-03-05 00:00:00

  • Exploring the subsite specificity of Schistosoma mansoni aspartyl hemoglobinase through comparative molecular modelling.

    abstract::Blood flukes of the genus Schistosoma currently infect millions of people in tropical and subtropical countries. An enzyme playing a major role in hemoglobin (Hb) degradation by Schistosoma mansoni has been cloned and shown to be highly similar to the human cathepsin D aspartyl proteinase, although presenting a distin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Silva FP Jr,Ribeiro F,Katz N,Giovanni-De-Simone S

    更新日期:2002-03-13 00:00:00

  • Human macrophage inflammatory protein-3alpha/CCL20/LARC/Exodus/SCYA20 is transcriptionally upregulated by tumor necrosis factor-alpha via a non-standard NF-kappaB site.

    abstract::The 5'-flanking sequences of the human macrophage inflammatory protein-3alpha/CCL20 gene were cloned and transfected into G-361 human melanoma cells in a luciferase reporter construct. Tumor necrosis factor-alpha (TNF-alpha) treatment stimulated luciferase expression, and promoter truncations demonstrated that TNF-alp...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Harant H,Eldershaw SA,Lindley IJ

    更新日期:2001-12-14 00:00:00

  • Mechanism of coupling periodate-oxidized nucleosides to proteins.

    abstract::This paper describes a mechanism of coupling periodate-oxidized nucleosides to proteins. Each of the dialdehyde groups of a periodate-oxidized nucleoside is shown to couple to lysine residues on different protein molecules through Schiff bases, thereby cross-linking different protein molecules, forming a polymer. This...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Senapathy P,Ali MA,Jacob MT

    更新日期:1985-10-14 00:00:00

  • Linkage between phosphorylated cystatin alpha and filaggrin by epidermal transglutaminase as a model of cornified envelope and inhibition of cathepsin L activity by cornified envelope and the conjugated cystatin alpha.

    abstract::Lysine-rich phosphorylated cystatin alpha (P-cystatin alpha) from newborn rat epidermis is a good substrate for epidermal transglutaminase (TGase) and also one of the component proteins of cornified envelope in the stratum corneum. Since the filaggrin linker segment peptide was efficiently conjugated with P-cystatin a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Takahashi M,Tezuka T,Kakegawa H,Katunuma N

    更新日期:1994-03-07 00:00:00

  • Regulation of ubiquitous 6-phosphofructo-2-kinase by the ubiquitin-proteasome proteolytic pathway during myogenic C2C12 cell differentiation.

    abstract::6-Phosphofructo-2-kinase catalyzes the synthesis and degradation of fructose 2,6-bisphosphate, activator of phosphofructokinase-1 and inhibitor of fructose 1,6-bisphosphatase. These properties confer to this bifunctional enzyme a key role in the control of glycolysis and gluconeogenesis. Several mammalian isozymes gen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Riera L,Obach M,Navarro-Sabaté A,Duran J,Perales JC,Viñals F,Rosa JL,Ventura F,Bartrons R

    更新日期:2003-08-28 00:00:00

  • A phosphorothioate oligonucleotide blocks reverse transcription via an antisense mechanism.

    abstract::We have studied the inhibition by a phosphorothioate oligodeoxynucleotide (17PScap) of cDNA synthesis performed by either avian or murine reverse transcriptase. Three different mechanisms of inhibition were identified: at low concentrations (< 100 nM), the cleavage of the RNA template by the retroviral RNase H at the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Boiziau C,Moreau S,Toulmé JJ

    更新日期:1994-03-07 00:00:00

  • The prostate-specific antigen gene and the human glandular kallikrein-1 gene are tandemly located on chromosome 19.

    abstract::Using a prostate-specific antigen cDNA as a hybridization probe, clones containing the kallikrein genes encoding prostate-specific antigen, human glandular kallikrein-1 and pancreas/kidney kallikrein were isolated from a human genomic library. Clones containing the prostate-specific antigen gene and the human glandula...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Riegman PH,Vlietstra RJ,Klaassen P,van der Korput JA,Geurts van Kessel A,Romijn JC,Trapman J

    更新日期:1989-04-10 00:00:00

  • Extended analyses of the Wnt/beta-catenin pathway: robustness and oscillatory behaviour.

    abstract::The Wnt/beta-catenin signalling pathway is evolutionarily conserved across many species and plays important roles during embryogenesis. The Lee-Heinrich model (to recognize the contributions of R. Heinrich we will refer to the work proposed by Lee et al. [Lee, E., Salic, A., Krüger, R., Heinrich, R., Kirschner, M.W. (...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wawra C,Kühl M,Kestler HA

    更新日期:2007-08-21 00:00:00

  • Knock-out of the cyaY gene in Escherichia coli does not affect cellular iron content and sensitivity to oxidants.

    abstract::Friedreich ataxia is a recessively inherited neurodegenerative disease caused by deficiency of a highly conserved mitochondrial protein, frataxin. Frataxin deficiency results in mitochondrial iron accumulation and oxidative stress. Frataxin shows homology with the CyaY proteins of gamma-purple bacteria, whose function...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Li DS,Ohshima K,Jiralerspong S,Bojanowski MW,Pandolfo M

    更新日期:1999-07-30 00:00:00

  • Oligo(3'-deoxy ADP-ribosyl)ation of the nuclear matrix lamins from rat liver utilizing 3'-deoxyNAD as a substrate.

    abstract::It has previously been shown that the levels of poly(ADP-ribose)polymerase and polymers of ADP-ribose that co-purify with the nuclear matrix in regenerating liver fluctuate with the levels of in vivo DNA replication [(1988) FEBS Lett. 236, 362-366]. We have now electrophoretically identified lamins A and C, and poly(A...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pedraza-Reyes M,Alvarez-Gonzalez R

    更新日期:1990-12-17 00:00:00

  • Constitutive association of JAK1 and STAT5 in pro-B cells is dissolved by interleukin-4-induced tyrosine phosphorylation of both proteins.

    abstract::The bipartite human interleukin-4 (IL-4) receptor was functionally expressed in murine pro-B cells and activated by human IL-4 to evoke intracellular signaling. Mutual association of signal transducing proteins within the receptor complex was then studied in dependence of ligand stimulation. Besides ligand-induced rec...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Erhardt I,Lischke A,Sebald W,Friedrich K

    更新日期:1998-11-13 00:00:00

  • NGF-dependent and tissue-specific transcription of vgf is regulated by a CREB-p300 and bHLH factor interaction.

    abstract::Neurotrophins support neuronal survival, development, and plasticity through processes requiring gene expression. We studied how vgf target gene transcription is mediated by a critical promoter region containing E-box, CCAAT and cAMP response element (CRE) sites. The p300 acetylase was present in two distinct protein ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mandolesi G,Gargano S,Pennuto M,Illi B,Molfetta R,Soucek L,Mosca L,Levi A,Jucker R,Nasi S

    更新日期:2002-01-02 00:00:00

  • Pneumococcal lipoproteins involved in bacterial fitness, virulence, and immune evasion.

    abstract::Streptococcus pneumoniae (pneumococcus) has evolved sophisticated strategies to survive in several niches within the human body either as a harmless commensal or as a serious pathogen causing a variety of diseases. The dynamic interaction between pneumococci and resident host cells during colonization of the upper res...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Kohler S,Voß F,Gómez Mejia A,Brown JS,Hammerschmidt S

    更新日期:2016-11-01 00:00:00

  • A role for a pertussis toxin-sensitive trimeric G-protein in store-operated Ca2+ inflow in hepatocytes.

    abstract::The mechanism of store-operated Ca2+ inflow in hepatocytes was investigated using fluo-3 and fura-2 to monitor changes in the concentration of intracellular free Ca2+ in single cells, and 1-(alpha-glycerophosphoryl)-myo-inositol 4,5-diphosphate, P4(5)-1-(2-nitrophenyl)ethyl ester ('caged' GPIP2) and 'caged' guanosine ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Berven LA,Barritt GJ

    更新日期:1994-06-13 00:00:00

  • Functional analysis of the conserved histidine residue of Bamboo mosaic virus capping enzyme in the activity for the formation of the covalent enzyme-m7GMP intermediate.

    abstract::The alphavirus-like mRNA capping enzyme of Bamboo mosaic virus (BaMV) exhibits an AdoMet-dependent guanylyltransferase activity by which the methyl group of AdoMet is transferred to GTP, leading to the formation of m(7)GTP, and the m(7)GMP moiety is next transferred to the 5' end of ppRNA via a covalent enzyme-m(7)GMP...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lin HY,Yu CY,Hsu YH,Meng M

    更新日期:2012-07-30 00:00:00

  • Molybdate transport through the plant sulfate transporter SHST1.

    abstract::Molybdenum is an essential micronutrient required by plants. The mechanism of molybdenum uptake in plants is poorly understood, however, evidence has suggested that sulfate transporters may be involved. The sulfate transporter from Stylosanthes hamata, SHST1, restored growth of the sulfate transport yeast mutant, YSD1...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fitzpatrick KL,Tyerman SD,Kaiser BN

    更新日期:2008-04-30 00:00:00

  • A low rate of nucleotide changes in Escherichia coli K-12 estimated from a comparison of the genome sequences between two different substrains.

    abstract::Two genome sequences of Escherichia coli K-12 substrains, one partial W3110 and one complete MG1655, have been determined by Japanese and American genome projects, respectively. In order to estimate the rate of nucleotide changes, we directly compared 2 Mb of the nucleotide sequences from these closely-related E. coli...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Itoh T,Okayama T,Hashimoto H,Takeda J,Davis RW,Mori H,Gojobori T

    更新日期:1999-04-30 00:00:00

  • Direct protein-protein interactions and substrate channeling between cellular retinoic acid binding proteins and CYP26B1.

    abstract::Cellular retinoic acid binding proteins (CRABPs) bind all-trans-retinoic acid (atRA) tightly. This study aimed to determine whether atRA is channeled directly to cytochrome P450 (CYP) CYP26B1 by CRABPs, and whether CRABPs interact directly with CYP26B1. atRA bound to CRABPs (holo-CRABP) was efficiently metabolized by ...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Nelson CH,Peng CC,Lutz JD,Yeung CK,Zelter A,Isoherranen N

    更新日期:2016-08-01 00:00:00

  • Gcm1, a mammalian homolog of Drosophila glial cells missing.

    abstract::Differentiation of glia (astrocytes and oligodendrocytes) in Drosophila requires the gene glial cells missing (gcm), which controls lineage determination. In the absence of gcm, neuroglia progenitors exclusively differentiate into neurons, instead of into both neurons and glia. In contrast, ectopic overexpression of g...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Altshuller Y,Copeland NG,Gilbert DJ,Jenkins NA,Frohman MA

    更新日期:1996-09-16 00:00:00

  • Construction of a fully active truncated alternansucrase partially deleted of its carboxy-terminal domain.

    abstract::Recombinant expression of the large alternansucrase (2057 amino acids) was hindered in E. coli due to poor enzyme solubility and protein degradation. The effects of deletions of the alternansucrase C-terminal CW-like and APY repeated motifs on enzyme solubility and specificity were investigated. A truncated variant de...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Joucla G,Pizzut S,Monsan P,Remaud-Simeon M

    更新日期:2006-02-06 00:00:00

  • The expression of the H19 gene and its function in human bladder carcinoma cell lines.

    abstract::The human H19 gene is a paternally imprinted oncofetal gene, highly expressed in several fetal tissues, down-regulated in nearly all adult tissues but re-expressed in carcinomas of tissues which express the gene in fetal life. It has no known protein product and till today, no function could be designated to H19 RNA. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ohana P,Kopf E,Bibi O,Ayesh S,Schneider T,Laster M,Tykocinski M,de Groot N,Hochberg A

    更新日期:1999-07-02 00:00:00

  • Down-regulation of the protein kinase A pathway by activators of protein kinase C and intracellular Ca2+ in fibroblast cells.

    abstract::Many genes are regulated by the intracellular calcium, protein kinase C (PKC) and protein kinase A (PKA) pathways and it has been shown that these pathways synergize in some cell types, whereas they antagonize in others. Here we show that the calcium and PKC pathways suppress the effects mediated by the PKA pathway in...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Döbbeling U,Berchtold MW

    更新日期:1996-08-05 00:00:00

  • Transient small molecule interactions kinetically modulate amyloid β peptide self-assembly.

    abstract::Small organic molecules, like Congo red and lacmoid, have been shown to modulate the self-assembly of the amyloid β peptide (Aβ). Here, we show that Aβ forms NMR invisible non-toxic co-aggregates together with lacmoid as well as Congo red. We find that the interaction involves two distinct kinetic processes and at eve...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Abelein A,Lang L,Lendel C,Gräslund A,Danielsson J

    更新日期:2012-11-16 00:00:00

  • Release of the FAD domain from cellobiose oxidase by proteases from cellulolytic cultures of Phanerochaete chrysosporium.

    abstract::Evidence has previously suggested that cellobiose:quinone oxidoreductase (CBQ) in cellulolytic cultures of Phanerochaete chrysosporium might be produced from cellobiose oxidase (CBO) by proteolytic cleavage. This study demonstrates that the ratio of CBO activity to (CBO + CBQ) activity declines with decreasing culture...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Habu N,Samejima M,Dean JF,Eriksson KE

    更新日期:1993-07-26 00:00:00

  • The amino-terminal domain of thrombomodulin and pancreatic stone protein are homologous with lectins.

    abstract::Amino acid sequence alignment of the amino-terminal of thrombomodulin and pancreatic stone protein with the hepatic asialoglycoprotein receptor shows that these proteins are homologous. From the known disulfide bridge pattern of other proteins belonging to the same family two disulfide bonds can be predicted. The homo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Petersen TE

    更新日期:1988-04-11 00:00:00

  • Bradykinin-induced transient accumulation of inositol trisphosphate in neuron-like cell line NG108-15 cells.

    abstract::Studies were undertaken to further elucidate the mechanism(s) by which bradykinin-dependent phosphoinositide metabolism takes place in neuroblastoma X glioma hybrid NG108-15 cells [(1984) J. Biol. Chem. 259, 10201-10207] using [3H]inositol-labelled cells. Bradykinin produced net increases in the level of [3H]inositol ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yano K,Higashida H,Hattori H,Nozawa Y

    更新日期:1985-02-25 00:00:00

  • The GIY-YIG endonuclease domain of Arabidopsis MutS homolog 1 specifically binds to branched DNA structures.

    abstract::In plant organelle genomes, homeologous recombination between heteroallelic positions of repetitive sequences is increased by dysfunction of the gene encoding MutS homolog 1 (MSH1), a plant organelle-specific homolog of bacterial mismatch-binding protein MutS1. The C-terminal region of plant MSH1 contains the GIY-YIG ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fukui K,Harada A,Wakamatsu T,Minobe A,Ohshita K,Ashiuchi M,Yano T

    更新日期:2018-12-01 00:00:00