Abstract:
:Human immunodeficiency virus type 1 (HIV-1) Rev is a small RNA-binding protein which is essential for viral replication. To investigate the structure of Rev we have mapped the binding sites of a panel of monoclonal antibodies (mAb) by protein footprinting and identified a mAb protecting amino acids within both the N- and C-terminal parts of Rev. Our mapping results support a previously proposed structure (Auer et al., Biochemistry, 33 (1994) 2988-2996) predicting that a helix-loop-helix motif in Rev brings the termini of the protein into proximity. Furthermore, we demonstrate that the binding sites mapped by protein footprinting are in agreement with conventional epitope mapping results and that this technique provides an advantageous strategy for mapping discontinuous sites.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Jensen TH,Jensen A,Szilvay AM,Kjems Jdoi
10.1016/s0014-5793(97)00988-5subject
Has Abstractpub_date
1997-09-01 00:00:00pages
50-4issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(97)00988-5journal_volume
414pub_type
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