Crystal structure of a caricain D158E mutant in complex with E-64.

abstract：:Addition of hydrocortisone to the rat mammary gland myoepithelial cell line, G4.2.3, induces the synthesis and secretion of alpha 2-macroglobulin. Interleukin-1 beta, interleukin-6 and transforming growth factor-beta synergize with hydrocortisone, increasing the synthesis of alpha 2-macroglobulin 2- to 4-fold, althoug...

journal_title：FEBS letters

authors： Warburton MJ,Gusterson BA,O'Hare MJ

更新日期：1993-10-11 00:00:00

abstract：:Apolipoprotein E- (ApoE-) rich high-density lipoprotein (HDL), which was prepared from the bound fraction of normolipemic volunteers on heparin-Sepharose and from a hyperalphalipoproteinemic patient, potently inhibited aggregation of human platelets in a dose-dependent fashion. Dimyristoyl phosphatidylcholine liposome...

journal_title：FEBS letters

authors： Higashihara M,Kinoshita M,Teramoto T,Kume S,Kurokawa K

更新日期：1991-04-22 00:00:00

abstract：:Msn2 and Msn4 transcription factors activate expression of stress-responsive element (STRE) controlled genes in response to various stresses triggering the environmental stress response in Saccharomyces cerevisiae. Although high hydrostatic pressure is known to induce gene expression modification in yeast, the transcr...

journal_title：FEBS letters

authors： Domitrovic T,Fernandes CM,Boy-Marcotte E,Kurtenbach E

更新日期：2006-11-13 00:00:00

abstract：:2-( Dimethylaminostyryl )-l-methyl pyridinium, a fluorescent indicator of mitochondrial membrane potential, is accumulated up to 400-fold by intact, isolated brown adipocytes from cold-adapted guinea pigs. The indicator can be optically localized within the in situ mitochondria. Ionophores which would be predicted to ...

journal_title：FEBS letters

authors： Rafael J,Nicholls DG

更新日期：1984-05-07 00:00:00

abstract：:The solution structure of the catalytic domain of MMP-20, a member of the matrix metalloproteinases family not yet structurally characterized, complexed with N-Isobutyl-N-(4-methoxyphenylsulfonyl)glycyl hydroxamic acid (NNGH), is here reported and compared with other MMPs-NNGH adducts. The backbone dynamic has been ch...

journal_title：FEBS letters

authors： Arendt Y,Banci L,Bertini I,Cantini F,Cozzi R,Del Conte R,Gonnelli L

更新日期：2007-10-02 00:00:00

abstract：:The mitochondrial permeability transition pore, a cyclosporin A-sensitive channel, is controlled by the transmembrane electric potential difference across the inner membrane. Here, we show that treatment of rat liver mitochondria with the arginine reagent phenylglyoxal inhibits the permeability transition pore trigger...

journal_title：FEBS letters

authors： Eriksson O,Fontaine E,Petronilli V,Bernardi P

更新日期：1997-06-16 00:00:00

abstract：:Treatment of 125I-labelled high-density lipoprotein ([125I]HDL3) with monospecific polyclonal antibodies against apolipoproteins A-I and A-II resulted in a dose-dependent inhibition of the [125I]HDL3 binding to isolated human small intestine epithelial cells by 25% and 50%, respectively. Both antibodies also inhibited...

journal_title：FEBS letters

authors： Sviridov DD,Ehnholm C,Tenkanen H,Pavlov MYu,Safonova IG,Repin VS

更新日期：1992-06-01 00:00:00

abstract：:In endocrine cells the regulated secretion of hormones, peptides, enzymes and neurotransmitters into the external medium occurs when mature secretory granules fuse with the plasma membrane. Secretory granules form at the trans-Golgi network (TGN) by envelopment of the dense-core aggregate of regulated secretory protei...

journal_title：FEBS letters

authors： Tooze SA

更新日期：1991-07-22 00:00:00

abstract：:Genome maintenance and integrity requires continuous alterations of the compaction state of the chromatin structure. Chromatin remodelers, among others the INO80 complex, help organize chromatin by repositioning, reshaping, or evicting nucleosomes. We report on INO80 nucleosome remodeling, assayed by single-molecule F...

journal_title：FEBS letters

authors： Schwarz M,Schall K,Kallis E,Eustermann S,Guariento M,Moldt M,Hopfner KP,Michaelis J

更新日期：2018-02-01 00:00:00

abstract：:Inducible heat shock protein 70 (Hsp70) is one of the most important HSPs for maintenance of cell integrity during normal cellular growth as well as pathophysiological conditions. Tumor necrosis factor receptor-associated factor 6 (TRAF6) is a crucial signaling transducer that regulates a diverse array of physiologica...

journal_title：FEBS letters

authors： Chen H,Wu Y,Zhang Y,Jin L,Luo L,Xue B,Lu C,Zhang X,Yin Z

更新日期：2006-05-29 00:00:00

abstract：:A method was established for measuring molecule numbers of three different myosin heavy chain (MHC) mRNA isoforms in total RNA preparations. The quantification was based on a combination of primer-directed reverse transcriptase and polymerase chain reactions with 5'-digoxigenin-labeled oligonucleotides, using external...

journal_title：FEBS letters

authors： Peuker H,Pette D

更新日期：1993-03-08 00:00:00

abstract：:Kinetic analyses of cpTat-mediated protein transport across the thylakoid membrane were conducted, revealing three important characteristics of this translocation pathway. First, transport via the cpTAT system displays a non-Michaelis-Menten, sigmoidal rate-substrate relationship with an apparent Hill coefficient of 1...

journal_title：FEBS letters

authors： Alder NN,Theg SM

更新日期：2003-04-10 00:00:00

abstract：:The molecular mass of the rabbit prolactin receptor (rbPRLR) deduced from cDNA cloning is 66 kDa. However, the molecular mass of the full-length receptor expressed in the insect Sf9 cells was found to be 94 kDa. In order to explain this discrepancy, we analyzed the possible post-translational modifications of the PRLR...

journal_title：FEBS letters

authors： Cahoreau C,Garnier L,Djiane J,Devauchelle G,Cerutti M

更新日期：1994-08-22 00:00:00

abstract：:Gastric H+,K+-ATPase activity is not affected by Na+ at pH 7.0 but is significantly stimulated by Na+ at pH 8.5. For the stimulation at the latter pH, the presence of both Na+ and K+ were essential. Contrary the H+,K+-ATPase, the associated K+-pNPPase was inhibited by Na+ at both pH values. Sodium competes with K+ for...

journal_title：FEBS letters

authors： Ray TK,Nandi J

更新日期：1985-06-03 00:00:00

abstract：:Under destabilising conditions both heat and cold denaturation of yeast phosphoglycerate kinase (PGK) can be observed. According to previous interpretation of experimental data and theoretical calculations, the C-terminal domain should be more stable than the N-terminal domain at all temperatures. We report on thermal...

journal_title：FEBS letters

authors： Gast K,Damaschun G,Desmadril M,Minard P,Müller-Frohne M,Pfeil W,Zirwer D

更新日期：1995-01-30 00:00:00

abstract：:Reliably pinpointing which specific amino acid residues form the interface(s) between a protein and its binding partner(s) is critical for understanding the structural and physicochemical determinants of protein recognition and binding affinity, and has wide applications in modeling and validating protein interactions...

journal_title：FEBS letters

authors： Xue LC,Dobbs D,Bonvin AM,Honavar V

更新日期：2015-11-30 00:00:00

abstract：:A cell culture model of blood-brain barrier (BBB, coculture of rat brain endothelial cells with rat astrocytes) was used to investigate the effect of nitric oxide (.NO) on the damage of the BBB induced by hypoxia/reoxygenation (H/R). Permeability coefficient of fluorescein across the endothelium was used as a marker o...

journal_title：FEBS letters

authors： Utepbergenov DI,Mertsch K,Sporbert A,Tenz K,Paul M,Haseloff RF,Blasig IE

更新日期：1998-03-13 00:00:00

abstract：:Natural freeze tolerance depends on cellular adaptations that address the multiple stresses imposed on cells during freezing. These adaptations preserve viability by suppressing energy-expensive cell processes in the frozen state. In this study, we explore the freeze-responsive expression of microRNA in hatchling pain...

journal_title：FEBS letters

authors： Biggar KK,Storey KB

更新日期：2015-11-30 00:00:00

abstract：:Higher basal 2-deoxy-D-glucose uptake in rheumatoid synovial cells than in non-rheumatoid synovial cells, was found to be associated with an increased interleukin-1 beta (IL-1 beta) secretion (respectively 850 +/- 238 vs. 8.3 +/- 2.4 pg/24 h/10(5) cells, mean +/- S.E.M.). When exogenous human recombinant IL-1 beta was...

journal_title：FEBS letters

authors： Hernvann A,Aussel C,Cynober L,Moatti N,Ekindjian OG

更新日期：1992-05-25 00:00:00

abstract：:Pro-apoptotic functions of the BH3-only protein BAD are negatively regulated by survival signal-mediated phosphorylation at several serine residues. Recently, we found that the mutant BAD (BADD119G) with an amino acid substitution of Asp (Asp119 to Gly) within the BH3 domain displays strong pro-apoptotic activity in s...

journal_title：FEBS letters

authors： Adachi M,Zhang YB,Imai K

更新日期：2003-09-11 00:00:00

abstract：:The influence of duplex stabilizing oligonucleotide-effector (oligonucleotide, carrying N-(2-hydroxyethyl)phenazinium residues on both ends), on selectivity of site-directed modification of E. coli 16 S rRNA (1542 nucleotides in length) under the conditions of its secondary structure stability was studied. The constan...

journal_title：FEBS letters

authors： Zenkova MA,Fedorova OS,Levina AS,Mamaev SV,Karpova GG

更新日期：1990-08-20 00:00:00

abstract：:Fucosyloligosaccharides have great therapeutic potential. Here we present a new route for synthesizing a Fucalpha1,2Gal linkage by introducing glycosynthase technology into 1,2-alpha-l-fucosidase. The enzyme adopts a unique reaction mechanism, in which asparagine-423 activated by aspartic acid-766 acts as a base while...

journal_title：FEBS letters

authors： Wada J,Honda Y,Nagae M,Kato R,Wakatsuki S,Katayama T,Taniguchi H,Kumagai H,Kitaoka M,Yamamoto K

更新日期：2008-11-12 00:00:00

abstract：:Several recombinant proteins in inclusion bodies expressed in Escherichia coli have been measured by Fourier transform infrared and solid-state nuclear magnetic resonance spectra to provide the secondary structural characteristics of the proteins from hyperthermophilic archaeon Pyrococcus horikoshii OT3 (hyperthermoph...

journal_title：FEBS letters

authors： Umetsu M,Tsumoto K,Ashish K,Nitta S,Tanaka Y,Adschiri T,Kumagai I

更新日期：2004-01-16 00:00:00

abstract：:The rate at which dinucleoside phosphates are cleaved by RNases is supposed to be determined by the mole fraction of enzyme-substrate complexes in which the phosphodiester moiety of a dinucleoside phosphate has a highly reactive conformation. The mole fraction of such complexes for a particular RNase depends on the na...

journal_title：FEBS letters

authors： Yakovlev GI,Bocharov AL,Moiseyev GP,Mikhaylov SN

更新日期：1985-01-07 00:00:00

abstract：:The interaction of the P-beta-Cat(19-44) peptide, a 26 amino acid peptide (K(19)AAVSHWQQQSYLDpSGIHpSGATTTAP(44)) that mimics the phosphorylated beta-Catenin antigen, has been studied with its monoclonal antibody BC-22, by transferred nuclear Overhauser effect NMR spectroscopy (TRNOESY) and saturation transfer differen...

journal_title：FEBS letters

authors： Megy S,Bertho G,Gharbi-Benarous J,Baleux F,Benarous R,Girault JP

更新日期：2006-10-02 00:00:00

abstract：:Membrane incorporation and aggregation of the peptide alamethicin have been investigated as a function of lipid type. Head group and acyl chain regions both contribute to modulate alamethicin incorporation. Specifically, the peptide prefers thin membranes and saturated chains; incorporation is reduced by the presence ...

journal_title：FEBS letters

authors： Stankowski S,Schwarz G

更新日期：1989-07-03 00:00:00

abstract：:F-box/LRR-repeat protein 2 (FBXL2), a component of Skp-Cullin-F box (SCF) ubiquitin E3 ligase, has been shown to inhibit tumorigenesis by targeting and ubiquitinating several oncoproteins. However, its role in gastric cancer remains poorly understood. Here, by tandem mass spectrometry, we show that FBXL2 interacts wit...

journal_title：FEBS letters

authors： Li LQ,Pan D,Chen H,Zhang L,Xie WJ

更新日期：2016-02-01 00:00:00

abstract：:The CO-binding reaction of cytochrome P-450cam bound with (1R)-camphor and (1S)-camphor are compared in the temperature region of 210-260 K using time-resolved Fourier-transform infrared spectroscopy with the CO stretch vibration as spectroscopic probe. For (1S)-camphor as substrate the association of CO is slowed dow...

journal_title：FEBS letters

authors： Contzen J,Ristau O,Jung C

更新日期：1996-03-25 00:00:00

abstract：:Maurotoxin (MTX) is a 34-residue toxin that has been isolated from the venom of the chactidae scorpion Scorpio maurus palmatus, and characterized. Together with Pi1 and HsTx1, MTX belongs to a family of short-chain four-disulfide-bridged scorpion toxins acting on potassium channels. However, contrary to other members ...

journal_title：FEBS letters

authors： Carlier E,Fajloun Z,Mansuelle P,Fathallah M,Mosbah A,Oughideni R,Sandoz G,Di Luccio E,Geib S,Regaya I,Brocard J,Rochat H,Darbon H,Devaux C,Sabatier JM,de Waard M

更新日期：2001-02-02 00:00:00

abstract：:We have previously shown that Docetaxel-induced variable degrees of apoptosis in melanoma. In this report, we studied the beta-tubulin repertoire of melanoma cell lines and show that class III beta-tubulin expression correlated with Docetaxel-resistance. Sensitive cells showed low levels of class III beta-tubulin with...

journal_title：FEBS letters

authors： Mhaidat NM,Thorne RF,de Bock CE,Zhang XD,Hersey P

更新日期：2008-01-23 00:00:00