Abstract:
:Genome maintenance and integrity requires continuous alterations of the compaction state of the chromatin structure. Chromatin remodelers, among others the INO80 complex, help organize chromatin by repositioning, reshaping, or evicting nucleosomes. We report on INO80 nucleosome remodeling, assayed by single-molecule Foerster resonance energy transfer on canonical nucleosomes as well as nucleosomes assembled from tailless histones. Nucleosome repositioning by INO80 is a processively catalyzed reaction. During the initiation of remodeling, probed by the INO80 bound state, the nucleosome reveals structurally heterogeneous states for tailless nucleosomes (in contrast to wild-type nucleosomes). We, therefore, propose an altered energy landscape for the INO80-mediated nucleosome sliding reaction in the absence of histone tails.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Schwarz M,Schall K,Kallis E,Eustermann S,Guariento M,Moldt M,Hopfner KP,Michaelis Jdoi
10.1002/1873-3468.12973subject
Has Abstractpub_date
2018-02-01 00:00:00pages
318-331issue
3eissn
0014-5793issn
1873-3468journal_volume
592pub_type
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