Abstract:
:The accumulation of protoporphyrin IX (Proto IX) in light-sensitive mutants of Escherichia coli was detected by spectrofluorimetry. Fluorescence emission and excitation spectra were recorded from extracts of bacterial cells. Proto IX clearly accumulated in cells with mutations in the visA (hemH) gene but not in the wild-type strain CA274 or in visA mutants that had been rendered light-resistant by introduction of the wild-type visA+ gene. Accumulation of Proto IX was also not observed in cells with a mutation in the visB gene. These results confirm the hypothesis that the sensitivity of the visA mutants to light is caused by the abnormal accumulation of Proto IX, a substrate of ferrochelatase, as the result of a genetic defect in the gene for ferrochelatase.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Miyamoto K,Nishimura K,Masuda T,Tsuji H,Inokuchi Hdoi
10.1016/0014-5793(92)81341-ikeywords:
subject
Has Abstractpub_date
1992-10-05 00:00:00pages
246-8issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(92)81341-Ijournal_volume
310pub_type
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