Abstract:
:A cDNA encoding a human liver UDPGT (HLUG 25) transcribed and translated in vitro showed that the encoded protein was synthesized as a precursor and was cleaved and glycosylated when dog pancreatic microsomes were present during translation. The UDPGT cDNA was transiently expressed in mammalian cell culture (COS-7 cells) resulting in the biosynthesis of a polypeptide of 52 kDa. This expressed UDPGT glycoprotein catalysed the glucuronidation of hyodeoxycholic acid forming an ether glucuronide. These results suggest that this UDPGT isoenzyme may be responsible for the glucuronidation of 6 alpha-hydroxy bile acids in human liver.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Fournel-Gigleux S,Jackson MR,Wooster R,Burchell Bdoi
10.1016/0014-5793(89)80111-5subject
Has Abstractpub_date
1989-01-30 00:00:00pages
119-22issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(89)80111-5journal_volume
243pub_type
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