Crystal structure of a single-chain trimer of human adiponectin globular domain.

abstract：:Monoamine oxidase B (MAO B) is an outer mitochondrial membrane protein that oxidizes arylalkylamine neurotransmitters and has been a valuable drug target for many neurological disorders. The 1.7 angstrom resolution structure of human MAO B shows the enzyme is dimeric with a C-terminal transmembrane helix protruding fr...

journal_title：FEBS letters

authors： Binda C,Hubálek F,Li M,Edmondson DE,Mattevi A

更新日期：2004-04-30 00:00:00

abstract：:The KlICL1 gene, encoding isocitrate lyase in Kluyveromyces lactis, is essential for ethanol utilization. Deletion analyses identified two functional promoter elements, CSRE-A and CSRE-B. Transcription is activated on ethanol, but not on glucose, glycerol or lactate. Expression depends on the KlCat8p transcription fac...

journal_title：FEBS letters

authors： Rodicio R,López ML,Cuadrado S,Cid AF,Redruello B,Moreno F,Heinisch JJ,Hegewald AK,Breunig KD

更新日期：2008-03-05 00:00:00

abstract：:In the present study, we investigated the roles and molecular mechanisms of miR-320a in human nasopharyngeal carcinoma (NPC). miR-320a expression was strongly reduced in NPC tissues and cell lines. Overexpression of miR-320a significantly suppressed NPC cell growth, migration, invasion and tumor growth in a xenograft ...

journal_title：FEBS letters

authors： Qi X,Li J,Zhou C,Lv C,Tian M

更新日期：2014-10-16 00:00:00

abstract：:Phosphoenolpyruvate carboxylases from various organisms contain two conserved lysine residues. In the C4 dicot Flaveria trinervia, one of these residues is Lys600. Converting this Lys600 to Arg600 or Thr600 mainly increased the Km values and but had minimal effect on the Vmax. The Km for PEP, Mg2+ increased by up to 3...

journal_title：FEBS letters

authors： Gao Y,Woo KC

更新日期：1995-11-13 00:00:00

abstract：:Drosophila PTEN (dPTEN) plays indispensable roles in the development of Drosophila melanogaster by controlling cell size and number. Although three potential spliced forms of dPTEN have been isolated, functional distinction among these forms remains elusive. In this study, we demonstrate that all spliced forms of dPTE...

journal_title：FEBS letters

authors： Maehama T,Kosaka N,Okahara F,Takeuchi K,Umeda M,Dixon JE,Kanaho Y

更新日期：2004-05-07 00:00:00

abstract：:Fibroblast growth factor-2 (FGF-2) is mitogenic for the human breast cancer cell line MCF-7; here we investigate some of the signaling pathways subserving this activity. FGF-2 stimulation of MCF-7 cells resulted in a global increase of intracellular tyrosine phosphorylation of proteins, particularly FGF receptor subst...

journal_title：FEBS letters

authors： Vercoutter-Edouart A,Lemoine J,Smart CE,Nurcombe V,Boilly B,Peyrat J,Hondermarck H

更新日期：2000-08-04 00:00:00

abstract：:An interpretation of alternative crystal structures of the erythropoietin receptor, with and without ligand, led to the proposal of a scissor-like mechanism of activation. This model has been propagated in the literature and is still being used to interpret crystal structures of related type-I cytokine receptors. Here...

journal_title：FEBS letters

authors： Corbett MS,Poger D,Mark AE

更新日期：2016-09-01 00:00:00

abstract：:Male germ cells specifically express paralogues of components of the general transcription apparatus including ALF a paralogue of TFIIAalpha/beta. We show that endogenous ALF is proteolytically cleaved to give alpha- and beta-subunits and we map the proteolytic cleavage site by mass spectrometry. Immunoprecipitations ...

journal_title：FEBS letters

authors： Catena R,Argentini M,Martianov I,Parello C,Brancorsini S,Parvinen M,Sassone-Corsi P,Davidson I

更新日期：2005-06-20 00:00:00

abstract：:Intrinsically unstructured proteins (IUPs) are common in various proteomes and occupy a unique structural and functional niche in which function is directly linked to structural disorder. The evidence that these proteins exist without a well-defined folded structure in vitro is compelling, and justifies considering th...

journal_title：FEBS letters

authors： Tompa P

更新日期：2005-06-13 00:00:00

abstract：:The influence of islet-activating protein (IAP), a Bordetella pertussis toxin, was studied on adenylate cyclase and GTPase activities in rat adipocyte membranes. Pretreatment of rats or intact rat adipocytes with IAP did not affect adenylate cyclase inhibition by the stable GTP analog, GTP gamma S, whereas inhibition ...

journal_title：FEBS letters

authors： Aktories K,Schultz G,Jakobs KH

更新日期：1983-05-30 00:00:00

abstract：:Lymphotoxin-beta receptor (LTbetaR) is a member of the tumor necrosis factor receptor (TNFR) superfamily that activates nuclear factor-kappaB (NF-kappaB) through the IkappaB kinase (IKK) complex, the core of which is comprised of IKK1, IKK2 and NF-kappaB essential modulator (NEMO). We demonstrate here that the LTbetaR...

journal_title：FEBS letters

authors： Saitoh T,Nakano H,Yamamoto N,Yamaoka S

更新日期：2002-12-04 00:00:00

abstract：:In adipogenesis, growth factors play a crucial role. Using serum depleted condition, we studied the causal role of endothelin-1 (ET-1) and epidermal growth factor (EGF), separately or together, in adipocyte differentiation of 3T3-L1 cells. ET-1 stimulation caused an anti-adipogenic response and this effect was potenti...

journal_title：FEBS letters

authors： Bhattacharya I,Ullrich A

更新日期：2006-10-16 00:00:00

abstract：:Extracellular vesicles (EVs) contain microRNAs (miRNAs). However, the exact molecular mechanisms of the recruitment of miRNAs in EVs are not well characterized. Based on proteomic analysis, we identified that silencing of Annexin A2 (ANXA2) significantly decreased the amount of miRNAs in EVs. In addition, microarray a...

journal_title：FEBS letters

authors： Hagiwara K,Katsuda T,Gailhouste L,Kosaka N,Ochiya T

更新日期：2015-12-21 00:00:00

abstract：:The transmembrane protein MIG-13 is a key regulator required for anterior migration of neural cells in Caenorhabditis elegans, but the signaling mechanisms involved remain unknown. Here, we isolated a suppressor mutation in the unc-71/adm-1 gene, which rescued the AVM neuron migration defect in mig-13 mutants. Genetic...

journal_title：FEBS letters

authors： Masuda H,Nakamura K,Takata N,Itoh B,Hirose T,Moribe H,Mekada E,Okada M

更新日期：2012-03-23 00:00:00

abstract：:The majority of cases of early-onset familial Alzheimer disease are caused by mutations in the recently identified presenilin 1 (PS1) gene, located on chromosome 14. PS1, a 467 amino acid protein, is predicted to be an integral membrane protein containing seven putative transmembrane domains and a large hydrophilic lo...

journal_title：FEBS letters

authors： Mercken M,Takahashi H,Honda T,Sato K,Murayama M,Nakazato Y,Noguchi K,Imahori K,Takashima A

更新日期：1996-07-08 00:00:00

abstract：:Activation gating in KcsA is elicited by changes in intracellular proton concentration. Thompson et al. identified a charge cluster around the inner gate that plays a key role in defining proton activation in KcsA. Here, through functional and spectroscopic approaches, we confirmed the role of this charge cluster and ...

journal_title：FEBS letters

authors： Cuello LG,Cortes DM,Jogini V,Sompornpisut A,Perozo E

更新日期：2010-03-19 00:00:00

abstract：:Adrenomedullin, a potently hypotensive peptide isolated from human pheochromocytoma, is known to elicit a rise in cAMP levels within mammalian endothelial and smooth muscle cells. Until now, however, little has been known about the adrenomedullin receptor. Recently, a group called receptor activity-modifying proteins ...

journal_title：FEBS letters

authors： Kamitani S,Asakawa M,Shimekake Y,Kuwasako K,Nakahara K,Sakata T

更新日期：1999-04-01 00:00:00

abstract：:The isolation and primary structure of intestinal neuropeptide Y (NPY) is described. The peptide was purified from porcine intestinal extracts using a chemical assay and radioimmunoassay for NPY. The amino acid sequence of this peptide is: Tyr-Pro-Ser-Lys-Pro-Asp-Asn-Pro-Gly-Glu-Asp-Ala-Pro-Ala-Glu-Asp-Leu-Ala- Arg-Ty...

journal_title：FEBS letters

authors： Tatemoto K,Siimesmaa S,Jörnvall H,Allen JM,Polak JM,Bloom SR,Mutt V

更新日期：1985-01-01 00:00:00

abstract：:Intracellular Ca(2+) ([Ca(2+)](i)) changes were measured in cell bodies of cultured rat hippocampal neurones with the fluorescent indicator Fluo-3. In the absence of external Ca(2+), the cholinergic agonist carbachol (200 microM) and the sarcoendoplasmic reticulum Ca(2+) pump inhibitor thapsigargin (0.4 microM) both t...

journal_title：FEBS letters

authors： Bouron A

更新日期：2000-03-31 00:00:00

abstract：:Lysosomal beta-galactosidase precursor is processed to a mature form and associated with protective protein in lysosomes. In this study we used two cysteine protease proinhibitors, E64-d for cathepsins B, S, H, and L, and CA074Me for cathepsin B. They are converted intracellularly to active forms, E-64c and CA074, res...

journal_title：FEBS letters

authors： Okamura-Oho Y,Zhang S,Callahan JW,Murata M,Oshima A,Suzuki Y

更新日期：1997-12-15 00:00:00

abstract：:Histone acetyltransferase activity was measured in isolated nuclei during the synchronous cell cycle of the myxomycete Physarum polycephalum. Nuclei were incubated with [14C]acetyl-coenzyme A and an excess of exogenous calf thymus histones. The activity is periodic during the cell cycle; it rises during the S-phase to...

journal_title：FEBS letters

authors： Golderer G,Loidl P,Gröbner P

更新日期：1987-10-05 00:00:00

abstract：:A fusion gene was constructed consisting of heat labile enterotoxin chain B (LTB) of E. coli genetically linked at its C-terminus to the beta-subunit of human chorionic gonadotropin in translational fusion, under the control of tac promoter and LTB signal sequence. Expression of the fusion gene (about 5 microgram/ml) ...

journal_title：FEBS letters

authors： Pillai D,Dixit A,Alok D,Garg LC

更新日期：1996-05-27 00:00:00

abstract：:We show that inverted membrane vesicles from Corynebacterium glutamicum, a Gram-positive bacterium, are able to generate and maintain an electrochemical gradient of protons in response to the addition of NADH. This result indicates that the respiratory chain is intact and that the vesicles are reasonably impermeable t...

journal_title：FEBS letters

authors： Schrempp S,Bayan N,Shechter E

更新日期：1994-12-12 00:00:00

abstract：:A 26 kDa peptide has been cleaved from the C-terminus of the S1 heavy chain with formic acid. Cleavage occurs in the '50 kDa' domain probably at the Asp-600-Pro-601 bond. This fragment has been renatured in the presence of the A2 light chain and the 26 kDa(A2) complex shown to interact with actin in an ATP-sensitive m...

journal_title：FEBS letters

authors： Griffiths AJ,Trayer IP

更新日期：1989-01-02 00:00:00

abstract：:Thrombin is a multifunctional enzyme involved in coagulation, cell modulation and inflammation. We recently reported a novel membrane-associated prothrombin activator, abbreviated as MAPA, found in cultured fibroblasts and glial cell lines. In this study, we examined the physiological role of this enzyme. MAPA-like ac...

journal_title：FEBS letters

authors： Shikamoto Y,Shibusawa S,Okuyama I,Morita T

更新日期：1997-08-04 00:00:00

abstract：:Phosphorylated sites of calf thymus H2B histone were investigated with a catalytic fragment of 72 kDa protein-tyrosine kinase (p72syk). Three of five tyrosine residues in H2B histone can be phosphorylated by this kinase. In this analysis, H2B histone was thoroughly phosphorylated in vitro with [gamma-32P]ATP and the k...

journal_title：FEBS letters

authors： Sakai K,Tanaka Y,Asahi M,Shimomura R,Taniguchi T,Hashimoto E,Yamamura H

更新日期：1991-12-02 00:00:00

abstract：:The phosphate transporter Pho84 of Saccharomyces cerevisiae is predicted to contain 12 transmembrane (TM) regions, divided into two partially duplicated parts of 6 TM segments. The three-dimensional (3D) organization of the Pho84 protein has not yet been determined. However, the 3D crystal structure of the Escherichia...

journal_title：FEBS letters

authors： Lagerstedt JO,Voss JC,Wieslander A,Persson BL

更新日期：2004-12-17 00:00:00

abstract：:Latent transforming growth factor-beta 1 binding protein (LTBP) is a constituent of the latent high molecular weight complex of TGF-beta 1 in human platelets. In the present communication, we show that LTBP binds Ca2+ in its free form as well as in the latent TGF-beta 1 complex. The binding of Ca2+ induces a structura...

journal_title：FEBS letters

authors： Colosetti P,Hellman U,Heldin CH,Miyazono K

更新日期：1993-04-05 00:00:00

abstract：:We investigated in vitro effects of recombinant human thrombopoietin (TPO), or c-Mpl ligand, on human platelets. TPO induced rapid dose-dependent tyrosine phosphorylation of several proteins. We identified Janus tyrosine kinases, Tyk2 and JAK2, and a member of STAT (signal transducers and activators of transcription) ...

journal_title：FEBS letters

authors： Ezumi Y,Takayama H,Okuma M

更新日期：1995-10-23 00:00:00

abstract：:Human high- and low-Mr kininogens were shown to be potent inhibitors of cysteine proteinases such as cathepsin L and papain (Ki = 17-48 pM). A strong immunological cross-reaction between the kininogens and low-Mr alpha-cysteine proteinase inhibitor from human plasma was found. Comparison of partial amino acid sequence...

journal_title：FEBS letters

authors： Müller-Esterl W,Fritz H,Machleidt W,Ritonja A,Brzin J,Kotnik M,Turk V,Kellermann J,Lottspeich F

更新日期：1985-03-25 00:00:00