Abstract:
:Intracellular Ca(2+) ([Ca(2+)](i)) changes were measured in cell bodies of cultured rat hippocampal neurones with the fluorescent indicator Fluo-3. In the absence of external Ca(2+), the cholinergic agonist carbachol (200 microM) and the sarcoendoplasmic reticulum Ca(2+) pump inhibitor thapsigargin (0.4 microM) both transiently elevated [Ca(2+)](i). A subsequent addition of Ca(2+) into the bathing medium caused a second [Ca(2+)](i) change which was blocked by lanthanum (50 microM). Taken together, these experiments indicate that stores depletion can activate a capacitative Ca(2+) entry pathway in cultured hippocampal neurones and further demonstrate the existence of such a Ca(2+) entry in excitable cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Bouron Adoi
10.1016/s0014-5793(00)01340-5keywords:
subject
Has Abstractpub_date
2000-03-31 00:00:00pages
269-72issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(00)01340-5journal_volume
470pub_type
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