Abstract:
:Acylaminoacyl peptidase is a member of the prolyl oligopeptidase family. Amino acid sequence alignment suggests that the stabilization of the tetrahedral intermediate should be mediated by His507 rather than by a tyrosine residue found in the other family members of this serine peptidase group. The pH dependence of k(cat)/K(m) did not reveal any effect of His507. Substitution of an alanine for His507 gave the same bell-shaped pH rate profile with the same pK(a) values (7.0 and 8.7). However, the value of the rate constant was 85 times lower with the modified enzyme, which indicated that His507 is an important residue that is probably involved in the formation of the 3-dimensional structure.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kiss AL,Szeltner Z,Fülöp V,Polgár Ldoi
10.1016/j.febslet.2004.06.054keywords:
subject
Has Abstractpub_date
2004-07-30 00:00:00pages
17-20issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579304007987journal_volume
571pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::L-Type calcium channel was expressed in Xenopus laevis oocytes injected with RNAs coding for different cardiac Ca2+ channel subunits, or with total heart RNA. The effects of activation of protein kinase C (PKC) by the phorbol ester PMA (4 beta-phorbol 12-myristate 13-acetate) were studied. Currents through channels co...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80980-u
更新日期:1992-07-20 00:00:00
abstract::Mediatophore is a protein purified from the nerve terminal membranes of Torpedo electric organ. It confers to artificial membranes a calcium-dependent mechanism that translocates acetylcholine. When similar reconstitution experiments are applied to rat brain synaptosomal membranes they reveal the presence of mediatoph...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80475-7
更新日期:1988-06-20 00:00:00
abstract::Ycf1p function is regulated by casein kinase 2α, Cka1p, via phosphorylation of Ser251. Cka1p-mediated phosphorylation of Ycf1p is attenuated in response to high salt stress. Previous results from our lab suggest a role for Ycf1p in cellular resistance to salt stress. Here, we show that Ycf1p plays an important role in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.02.010
更新日期:2012-03-23 00:00:00
abstract::Chemokines play a key role in modulating leukocyte functions at sites of inflammation. To assess chondrocyte contribution to the chemotactic environment of inflamed joints the intracellular content of CC and CXC chemokines was investigated. IL-8, GROalpha, MCP-1, RANTES, MIP-1alpha and MIP-1beta expression was evaluat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00886-8
更新日期:1999-07-23 00:00:00
abstract::Persistent tumour necrosis factor alpha (TNF-alpha) exposure uncouples proximal T-cell receptor (TCR)-signalling events. Here, we demonstrate that chronic TNF-alpha exposure also attenuates signalling distal to the TCR, by specifically inhibiting Ca2+ influx evoked by thapsigargin in CD4+ T-cells. Mitogen-induced Ca2+...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.01.051
更新日期:2005-02-28 00:00:00
abstract::An essential component of the chromatin remodeling machinery is NURF (Nucleosome Remodeling Factor), the founding member of the ISWI family of chromatin remodeling complexes. In vertebrates and invertebrates alike, NURF has many important functions in chromatin biology including regulating transcription, establishing ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2011.09.003
更新日期:2011-10-20 00:00:00
abstract::Free radicals, including dopamine (DA)-oxidized metabolites, have long been implicated in pathogenesis of Parkinson's disease (PD). However, the relationships between such oxidative stresses and alpha-synuclein (alpha-S), a major constituent of Lewy bodies, remain unknown. In this study, we established neuronal cells ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.03.018
更新日期:2006-04-03 00:00:00
abstract::Phosphatidylinositol transfer proteins (PITP) are abundant cytosolic proteins originally identified because of their ability to act in vitro as specific transporters of phosphatidylinositol or phosphatidylcholine between membranes. However, the cellular function of mammalian PITP has remained enigmatic till recently. ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(97)00414-6
更新日期:1997-06-23 00:00:00
abstract::Niemann-Pick C (NPC) disease is a progressive neurological disorder in which cholesterol, gangliosides and bis-monoacylglycerol phosphate accumulate in late endosomes/lysosomes. This disease is caused by mutations in either the NPC1 or NPC2 gene. NPC1 and NPC2 are involved in egress of lipids, particularly cholesterol...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2006.06.008
更新日期:2006-10-09 00:00:00
abstract::The 14 kDa protein was purified from human platelets. It displays high cytotoxic activity to the human ACL cells at 10 M concentration (21.8+/-7.1%). Its N-terminal sequence is YAPQXQFGP-, being highly homologous to region 241-249 residues of the human Cls complement component. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00205-6
更新日期:1997-04-01 00:00:00
abstract::Abnormal retention of Delta F508 CFTR (cystic fibrosis transmembrane conductance regulator) in the endoplasmic reticulum is a major cause of cystic fibrosis (CF). We show that calnexin Delta 185-520 but not calnexin can partially reverse the mislocalization of Delta F508 CFTR. This 256-amino acid protein has neither t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03134-4
更新日期:2002-08-28 00:00:00
abstract::Acid extracts of rat pituitary neuro-intermediate lobes have been shown by ion-exchange chromatography and radio-immunoassay to contain predominantly the inactive derivatives of beta-endorphin, alpha, N-acetyl beta-endorphin 1-27 and alpha, N-acetyl beta-endorphin 1-26; the biologically active form, beta-endorphin 1-3...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80778-4
更新日期:1984-10-01 00:00:00
abstract::Human high- and low-Mr kininogens were shown to be potent inhibitors of cysteine proteinases such as cathepsin L and papain (Ki = 17-48 pM). A strong immunological cross-reaction between the kininogens and low-Mr alpha-cysteine proteinase inhibitor from human plasma was found. Comparison of partial amino acid sequence...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80322-7
更新日期:1985-03-25 00:00:00
abstract::Phosphorylcholine, phosphorylcreatine, lactate, glutathione, glycine and leucine were identified in living HeLa cells in the first study of this cell type by 1H spin echo NMR spectroscopy. The technique has the advantage that it is non-invasive, providing detailed structural information on individual species present i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80086-8
更新日期:1987-04-20 00:00:00
abstract::In unicellular species codon usage is determined by mutational biases and natural selection. Among prokaryotes, the influence of these factors is different if the genome is skewed towards AT or GC, since in AT-rich organisms translational selection is absent. On the other hand, in AT-rich unicellular eukaryotes the tw...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02644-8
更新日期:2001-07-20 00:00:00
abstract::Cytochrome c oxidase is a membrane-bound redox-driven proton pump that harbors two proton-transfer pathways, D and K, which are used at different stages of the reaction cycle. Here, we address the question if a D pathway with a modified energy landscape for proton transfer could take over the role of the K pathway whe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13679
更新日期:2020-03-01 00:00:00
abstract::Limb girdle muscular dystrophy type 2A is linked to a skeletal muscle-specific calpain isoform known as p94. Isolation of the intact 94-kDa enzyme has been difficult to achieve due to its rapid autolysis, and uncertainty has arisen over its Ca2+-dependence for activity. We have expressed a C-terminally truncated form ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03722-5
更新日期:2002-12-18 00:00:00
abstract::Keratins form an intracellular keratin filament network in keratinocytes. Point mutations in the epidermal keratins could lead to the disruption of keratin filament formation, developing skin diseases such as epidermolytic hereditary palmoplantar keratoderma (EHPPK). We found a G to A transition in keratin 9 (K9) cDNA...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00393-6
更新日期:1996-05-20 00:00:00
abstract::The nucleotide sequence of the genomic RNA of potato leafroll virus was determined and its genetic organization deduced. The RNA is 5882 nucleotides long and contains 6 open reading frames (ORFs) encoding proteins of 70, 70, 56, 28, 23 and 17 kDa. The putative genes for the coat protein (23 kDa) and the RNA-dependent ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80190-5
更新日期:1989-03-13 00:00:00
abstract::A recombinant antigen (p22-NS3), possessing putative HCV nucleocapsid protein (p22) and non-structural protein 3 (NS3) epitopes, was heavily expressed in E. coli and purified. The p22-NS3 purified recombinant antigen strongly reacts with sera containing human antibodies directed against p22 and NS3 providing a startin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80128-h
更新日期:1993-06-21 00:00:00
abstract::Rabbits ingesting 4-fluorophenylalanine are known to incorporate small amounts of this fluorinated amino acid into their proteins. Carbonic anhydrase I isolated from the erythrocytes of animals so maintained exhibits a well-resolved fluorine NMR signal for each phenylalanine in the sequence. The chemical shifts of mos...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80216-2
更新日期:1986-02-03 00:00:00
abstract::Endothelial monocyte-activating polypeptide II (EMAP II) is a proinflammatory cytokine and a chemoattractant for leukocytes. The mature cytokine is formed in apoptotic cells by cleavage of the precursor proEMAP II. Here we show that caspase-7 is capable of cleaving proEMAP II in vitro. A proEMAP II mutant, in which th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01777-9
更新日期:2000-01-21 00:00:00
abstract::Purified barley leaf ADP-glucose pyrophosphorylase, a key enzyme of the starch synthesis in the chloroplast stroma, was analysed with respect to its possible regulation by factors defining the metabolic/effector status of the chloroplast during light and dark conditions. The enzyme required 3-phosphoglyceric acid for ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00346-4
更新日期:1999-04-01 00:00:00
abstract::Lipid metabolism is extensively studied in Saccharomyces cerevisiae. Here, we report that revertant of glycogen synthase kinase mutation-1 (Rog1p) possesses monoacylglycerol (MAG) lipase activity in S. cerevisiae. The lipase activity of Rog1p was confirmed in two ways: through analysis of a strain with a double deleti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.11.019
更新日期:2015-01-02 00:00:00
abstract::Atrial natriuretic factor (ANF) and C-type natriuretic peptide (CNP)-activated guanylate cyclases are single-chain transmembrane-spanning proteins, containing both ligand binding and catalytic activities. In both proteins, ligand binding to the extracellular receptor domain activates the cytosolic catalytic domain, ge...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80408-m
更新日期:1993-12-13 00:00:00
abstract::The central nervous system of the pond snail, Lymnaea stagnalis, contains many large, identified neurons which can be easily manipulated making it an advantageous model system to elucidate in vivo the architecture of neuronal signal transduction pathways. We have isolated three cDNA clones encoding G protein alpha sub...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81474-z
更新日期:1992-12-21 00:00:00
abstract::TLR3 and TLR4 utilize adaptor TRIF to activate interferon regulatory factor 3 (IRF3), resulting in interferon β (IFN-β) production to mediate anti-viral infection. In this report, we analyzed the effect of two known phosphatidylinositol 3-kinase (PI3K) inhibitors LY294002 and wortmannin on LPS- and poly(I:C)-induced I...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.01.016
更新日期:2012-03-23 00:00:00
abstract::We have isolated cDNAs encoding the glucocorticoid receptor from an androgen-dependent mouse tumor, Shionogi Carcinoma 115. The nucleotide sequence of the receptor revealed two different forms, designated as SC-GR I and SC-GR II. Both forms have a one-base substitution in the DNA binding domain of the wild-type mouse ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81339-p
更新日期:1990-11-12 00:00:00
abstract::Motile flagella are crucial for human fertility and embryonic development. The distal tip of the flagellum is where growth and intra-flagellar transport are coordinated. In most model organisms, but not all, the distal tip includes a 'singlet region', where axonemal doublet microtubules (dMT) terminate and only comple...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13379
更新日期:2019-05-01 00:00:00
abstract::The structure of the Ala38 variant of yeast iso-1-cytochrome c, in which the previously unchanged Arg38 has been replaced, has been characterised by NMR. The NMR data indicate that the structure of the Ala38 variant is very similar to that of the wild type protein. In particular, the heme environment and interactions ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80678-v
更新日期:1991-06-24 00:00:00