Abstract:
:Lipid metabolism is extensively studied in Saccharomyces cerevisiae. Here, we report that revertant of glycogen synthase kinase mutation-1 (Rog1p) possesses monoacylglycerol (MAG) lipase activity in S. cerevisiae. The lipase activity of Rog1p was confirmed in two ways: through analysis of a strain with a double deletion of ROG1 and monoglyceride lipase YJU3 (yju3Δrog1Δ) and by site-directed mutagenesis of the ROG1 lipase motif (GXSXG). Rog1p is localized in both the cytosol and the nucleus. Overexpression of ROG1 in a ROG1-deficient strain resulted in an accumulation of reactive oxygen species. These results suggest that Rog1p is a MAG lipase that regulates lipid homeostasis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Vishnu Varthini L,Selvaraju K,Srinivasan M,Nachiappan Vdoi
10.1016/j.febslet.2014.11.019subject
Has Abstractpub_date
2015-01-02 00:00:00pages
23-30issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(14)00824-2journal_volume
589pub_type
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