Modulation of cardiac Ca2+ channels in Xenopus oocytes by protein kinase C.

abstract：:Protein-tyrosine phosphorylation and dephosphorylation are directly associated with cellular growth, signal transduction, and neoplastic transformation. Here we report the isolation of a complementary DNA (cDNA) clone encoding a novel protein-tyrosine phosphatase (PTP) from a human T cell PEER cDNA library. The predic...

journal_title：FEBS letters

authors： Adachi M,Sekiya M,Miyachi T,Matsuno K,Hinoda Y,Imai K,Yachi A

更新日期：1992-12-21 00:00:00

abstract：:We have studied the incorporation of [(14)C]serine and of [(3)H]sphingosine into sphingomyelin in the presence or absence of brefeldin A (BFA) in three different cell types. Administration of BFA (1 microgram/ml) to fibroblasts for 24 h increased the incorporation of label into sphingomyelin 1.5-3 fold compared with u...

journal_title：FEBS letters

authors： Sadeghlar F,Sandhoff K,van Echten-Deckert G

更新日期：2000-07-28 00:00:00

abstract：:The N-terminal processing of MADH from the bacterium T. versutus and the N-terminal heterogeneity of the isolated alpha subunit of the alpha 2 beta 2 protein complex was demonstrated by a combination of Edman sequence analysis of an electroblotted band, in situ digested with pyroglutamate aminopeptidase, and accurate ...

journal_title：FEBS letters

authors： Van Beeumen J,Van Driessche G,Huitema F,Duine JA,Canters GW

更新日期：1993-10-25 00:00:00

abstract：:The operation of the human red cell glucose transporter has been studied at normal and high hydrostatic pressure to identify the step(s) which involve a volume change. Pressure inhibited zero-trans and equilibrium exchange influx to similar extents, by decreasing the Vmax but not significantly changing the Km. The Bma...

journal_title：FEBS letters

authors： Thorne SD,Hall AC,Lowe AG

更新日期：1992-04-27 00:00:00

abstract：:We investigated the role of p53 in nuclear factor (NF)-kappaB dependent, platelet-activating factor (PAF)-induced vascular endothelial growth factor (VEGF) expression. Transfected NF-kappaB subunits in ECV304 cells increased the tumor necrosis factor-alpha promoter activity, which was completely inhibited by p53. Tran...

journal_title：FEBS letters

authors： Ko HM,Jung HH,Seo KH,Kang YR,Kim HA,Park SJ,Lee HK,Im SY

更新日期：2006-05-29 00:00:00

abstract：:The photosynthetic reaction center is one of the most complicated molecular complexes. Transducing photon energy to a transmembrane electrochemical potential difference for protons, it is the direct or indirect energy source for virtually all life. We show here that it operates in a simple, battery-like manner, with a...

journal_title：FEBS letters

authors： van Rotterdam BJ,Crielaard W,van Stokkum IH,Hellingwerf KJ,Westerhoff HV

更新日期：2002-01-02 00:00:00

abstract：:Tripeptidyl peptidase I (TPP-I) is a lysosomal enzyme that cleaves tripeptides from the N-terminus of polypeptides. A comparison of TPP-I amino acid sequences with sequences derived from an EST database suggested that TPP-I is identical to a pepstatin-insensitive carboxyl proteinase of unknown specificity which is mut...

journal_title：FEBS letters

authors： Vines DJ,Warburton MJ

更新日期：1999-01-25 00:00:00

abstract：:The modification of homeodomain-interacting protein kinase 2 (HIPK2) by small ubiquitin-like modifier 1 (SUMO-1) plays an important role in its targeting into the promyelocytic leukemia body, as well as in its differential interaction with binding partner, but the desumoylation of HIPK2 by SUMO-specific proteases is l...

journal_title：FEBS letters

authors： Kim YH,Sung KS,Lee SJ,Kim YO,Choi CY,Kim Y

更新日期：2005-11-07 00:00:00

abstract：:A chemokine receptor, CXCR4, and its endogenous ligand, stromal cell-derived factor-1 (SDF-1), have been recognized to be involved in the metastasis of several types of cancers. T140 analogs are peptidic CXCR4 antagonists composed of 14 amino acid residues that were previously developed as anti-HIV agents having inhib...

journal_title：FEBS letters

authors： Tamamura H,Hori A,Kanzaki N,Hiramatsu K,Mizumoto M,Nakashima H,Yamamoto N,Otaka A,Fujii N

更新日期：2003-08-28 00:00:00

abstract：:The heterogeneity of computationally predicted reaction fluxes in metabolic networks within a single flux state can be exploited to detect their significant flux backbone. Here, we disclose the backbone of Escherichia coli, and compare it with the backbones of other bacteria. We find that, in general, the core of the ...

journal_title：FEBS letters

authors： Güell O,Sagués F,Serrano MÁ

更新日期：2017-05-01 00:00:00

abstract：:Prohormone convertases (PCs) 1 and 2 are thought to mediate the proteolytic cleavage of many peptide precursors. Endogenous inhibitors of both PC1 and PC2 have now been identified; the 7B2 protein is a nanomolar inhibitor of PC2, while the novel protein proSAAS was recently reported to be a micromolar inhibitor of PC1...

journal_title：FEBS letters

authors： Cameron A,Fortenberry Y,Lindberg I

更新日期：2000-05-12 00:00:00

abstract：:We have isolated a full-length cDNA (HPAsn.6) for human placenta glycosylasparaginase using a 221-bp PCR amplified fragment containing rat liver asparaginase gene sequences. The deduced amino acid sequence from the human clone showed sequence identity to both the alpha and beta subunits of the rat enzyme. The human en...

journal_title：FEBS letters

authors： Fisher KJ,Tollersrud OK,Aronson NN Jr

更新日期：1990-09-03 00:00:00

abstract：:The importance of HNF1 binding proteins for intestinal aminopeptidase N expression was investigated using the Caco-2 cell-line. Aminopeptidase N promoter activity in Caco-2 cells depends on the HNF1 element (positions -85 to -58) and co-transfection with an HNF1 alpha expression vector demonstrates a direct activation...

journal_title：FEBS letters

authors： Olsen J,Laustsen L,Troelsen J

更新日期：1994-04-11 00:00:00

abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key glycolytic enzyme regulated by many diverse mechanisms. In this study we present evidence that GAPDH activity is stimulated in the presence of oxyhemoglobin (2.3-fold, P < 0.005). No stimulation was seen by myoglobin, and only slight stimulation (1.2-fold, not ...

journal_title：FEBS letters

authors： Brookes PS,Land JM,Clark JB,Heales SJ

更新日期：1997-10-13 00:00:00

abstract：:Conformational changes in the extradiscal regions of rhodopsin induced by illumination were investigated by modifying the visual pigment by mild treatment with cyanogen bromide prior to and after light exposure. Light induced an increased yield of cleavage of the Met bond 253-254 and a new cleavage at the Met bond 155...

journal_title：FEBS letters

authors： Pellicone C,Nullans G,Virmaux N

更新日期：1985-02-11 00:00:00

abstract：:The transmembrane protein MIG-13 is a key regulator required for anterior migration of neural cells in Caenorhabditis elegans, but the signaling mechanisms involved remain unknown. Here, we isolated a suppressor mutation in the unc-71/adm-1 gene, which rescued the AVM neuron migration defect in mig-13 mutants. Genetic...

journal_title：FEBS letters

authors： Masuda H,Nakamura K,Takata N,Itoh B,Hirose T,Moribe H,Mekada E,Okada M

更新日期：2012-03-23 00:00:00

abstract：:Human CBS1 is a methionine sulfoxide reductase of type B (MSRB) as it specifically reduced Met-R-SO in peptides with dithiothreitol or the thioredoxin system as reductants. Mutation C169S in the active site completely abolished enzymatic activity, while mutation W110A only reduced activity and C105S had no effect. Lik...

journal_title：FEBS letters

authors： Jung S,Hansel A,Kasperczyk H,Hoshi T,Heinemann SH

更新日期：2002-09-11 00:00:00

abstract：:Duchenne muscular dystrophy (DMD) is a fatal disorder caused by absence of functional dystrophin protein. Compensation in dystrophin-deficient (mdx) mice may be achieved by overexpression of its fetal paralogue, utrophin. Strategies to increase utrophin levels by stimulating promoter activity using small compounds are...

journal_title：FEBS letters

authors： Perkins KJ,Davies KE

更新日期：2018-06-01 00:00:00

abstract：:13CO NMR chemical shifts and 12CO infrared stretching frequencies have been measured for cytochrome P-450cam-CO in the presence of D-camphor and of various camphor analogues. A linear correlation between both parameters delta (13C) and v(CO) was found indicating that the steric and electrostatic interactions acting on...

journal_title：FEBS letters

authors： Legrand N,Bondon A,Simonneaux G,Jung C,Gill E

更新日期：1995-05-08 00:00:00

abstract：:N-Oligosaccharyltransferase catalyzes the N-glycosylation of asparagine residues of nascent polypeptide chains in the endoplasmic reticulum, a pathway highly conserved in all eukaryotes. An enzymatically active complex was isolated from microsomal membranes from Saccharomyces cerevisiae, which is composed of four prot...

journal_title：FEBS letters

authors： Knauer R,Lehle L

更新日期：1994-05-09 00:00:00

abstract：:Mammalian heart has been reported to express AC isozymes (types V and VI) that are inhibited by < microM [Ca2+]; avian heart has been reported to express adenylyl cyclase activity that is inhibited by < microM [Ca2]. We have used reverse transcription polymerase chain reaction (RT-PCR) to determine that type V and VI ...

journal_title：FEBS letters

authors： Yu HJ,Unnerstall JR,Green RD

更新日期：1995-10-23 00:00:00

abstract：:We present evidence that gastrin, binding to a G protein-coupled receptor, activates the p38-mitogen-activated protein kinase (MAPK) pathway. Blockage of protein kinase C (PKC) by GF109203X, depletion of intracellular calcium by thapsigargin or inhibition of Src family kinases by PP2 prevented p38-MAPK activation and ...

journal_title：FEBS letters

authors： Dehez S,Daulhac L,Kowalski-Chauvel A,Fourmy D,Pradayrol L,Seva C

更新日期：2001-05-04 00:00:00

abstract：:A structural tree for proteins and domains containing S-like beta-sheets has been constructed. An S-like beta-sheet is taken as a starting structure in modelling or as a root structure of the tree. Larger structures are obtained by a stepwise addition of beta-strands and/or alpha-helices to the root S-like beta-sheet ...

journal_title：FEBS letters

authors： Efimov AV

更新日期：1998-10-23 00:00:00

abstract：:The lateral diffusion of BK(Ca) channels was previously shown to be highly 'confined' in the COS-7 cell membrane. Here we report that the diffusion coefficient and the confinement area of BK(Ca) channel were significantly increased by the treatment of latrunculin A, an actin-depolymerizing agent, but not by microtubul...

journal_title：FEBS letters

authors： Won S,Lee BC,Park CS

更新日期：2011-07-21 00:00:00

abstract：:The WWE domain is often identified in proteins associated with ubiquitination or poly-ADP-ribosylation. Structural information about WWE domains has been obtained for the ubiquitination-related proteins, such as Deltex and RNF146, but not yet for the poly-ADP-ribose polymerases (PARPs). Here we determined the solution...

journal_title：FEBS letters

authors： He F,Tsuda K,Takahashi M,Kuwasako K,Terada T,Shirouzu M,Watanabe S,Kigawa T,Kobayashi N,Güntert P,Yokoyama S,Muto Y

更新日期：2012-11-02 00:00:00

abstract：:Treatment of neonatal rat islets of Langerhans with combined cytokines (interleukin-1beta 10(-10) M, tumour necrosis factor-alpha 10(-10) M, interferon-gamma 5 U/ml) led to extensive cell death, which was potentiated by Fas activation with the anti-Fas cytolytic antibody JO2. Pre-treatment with insulin (25 ng/ml) or i...

journal_title：FEBS letters

authors： Harrison M,Dunger AM,Berg S,Mabley J,John N,Green MH,Green IC

更新日期：1998-09-18 00:00:00

abstract：:Among DNA repair pathways, nucleotide excision repair (NER) is able to recognize and process a wide variety of DNA lesions. The NER mechanism can be summarized in two stages: incision/excision of the lesion and DNA repair synthesis. Here, we have assessed the repair synthesis activity of protein extracts from differen...

journal_title：FEBS letters

authors： Coudoré F,Calsou P,Salles B

更新日期：1997-09-15 00:00:00

abstract：:Dendritic cells (DC) were enriched from murine spleen by exploring their intermediate density and transient weak adherence. The isolated population contained excellent antigen presenting cells with high surface expression of major histocompatibility complex (MHC) class II determinants thus exhibiting crucial immunofun...

journal_title：FEBS letters

authors： Fischer HG,Eder C

更新日期：1995-10-09 00:00:00

abstract：:Transcarboxylase from Propionibacterium shermanii is a complex biotin-containing enzyme composed of 30 polypeptides of three different types. It is composed of six dimeric outer subunits associated with a central cylindrical hexameric subunit through 12 biotinyl subunits; three outer subunits on each face of the centr...

journal_title：FEBS letters

authors： Thornton CG,Kumar GK,Shenoy BC,Haase FC,Phillips NF,Park VM,Magner WJ,Hejlik DP,Wood HG,Samols D

更新日期：1993-09-13 00:00:00

abstract：:The kinetics of complexing inactivation at identical enzyme and inhibitor concentrations were analyzed and the equations of product generation were derived when the free enzyme concentration is great, larger or smaller than the dissociation constant of inhibitor, K(I). The kinetic constants can be obtained by fitting ...

journal_title：FEBS letters

authors： Wang MH,Zhao KY

更新日期：1997-08-04 00:00:00