Abstract:
:We have isolated a full-length cDNA (HPAsn.6) for human placenta glycosylasparaginase using a 221-bp PCR amplified fragment containing rat liver asparaginase gene sequences. The deduced amino acid sequence from the human clone showed sequence identity to both the alpha and beta subunits of the rat enzyme. The human enzyme is encoded as a 34.6 kDa polypeptide that is post-translationally processed to generate two subunits of approx. 19.5 (alpha) and 15 (beta) kDa. A charge enriched region is present at the predicted site where cleavage occurs. Using polyclonal antibodies against the alpha and beta subunits of rat liver asparaginase, we have shown that the human enzyme is similar in structure to the rat enzyme.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Fisher KJ,Tollersrud OK,Aronson NN Jrdoi
10.1016/0014-5793(90)81211-6subject
Has Abstractpub_date
1990-09-03 00:00:00pages
440-4issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(90)81211-6journal_volume
269pub_type
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