Expression of ATP binding cassette-transporter ABCG1 prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol.

abstract：:An efficient method is presented for the production of intact mammalian prion proteins and partial sequences thereof. As an illustration we describe the production of polypeptides comprising residues 23-231, 81-231, 90-231 and 121-231 of the human prion protein (hPrP). Polypeptides were expressed as histidine tail fus...

journal_title：FEBS letters

authors： Zahn R,von Schroetter C,Wüthrich K

更新日期：1997-11-17 00:00:00

abstract：:Fibroblast growth factor-2 (FGF-2) is mitogenic for the human breast cancer cell line MCF-7; here we investigate some of the signaling pathways subserving this activity. FGF-2 stimulation of MCF-7 cells resulted in a global increase of intracellular tyrosine phosphorylation of proteins, particularly FGF receptor subst...

journal_title：FEBS letters

authors： Vercoutter-Edouart A,Lemoine J,Smart CE,Nurcombe V,Boilly B,Peyrat J,Hondermarck H

更新日期：2000-08-04 00:00:00

abstract：:A strain of yeast, Hansenula mrakii, secretes a toxin that kills sensitive yeasts, such as Saccharomyces cerevisiae Monoclonal antibodies raised against the toxin had both binding and neutralizing activities. The toxin in culture media was isolated by an affinity column of monoclonal antibody. The toxin is a basic pol...

journal_title：FEBS letters

authors： Yamamoto T,Imai M,Tachibana K,Mayumi M

更新日期：1986-01-20 00:00:00

abstract：:Complementary (c) DNAs encoding a glycine receptor (GlyR) isomer were cloned from libraries constructed in lambda ZAPII with poly (A)+ RNA of neonatal rat spinal cord. Northern blot analysis revealed that RNA hybridized to the cloned cDNA is detectable only for a period of late embryonic/early postnatal stage of the s...

journal_title：FEBS letters

authors： Akagi H,Hirai K,Hishinuma F

更新日期：1991-04-09 00:00:00

abstract：:The p53 tumour suppressor protein is tightly controlled by the E3 ubiquitin ligase, mouse double minute 2 (MDM2), but maintains MDM2 expression as part of a negative feedback loop. We have identified the immunophilin, 25kDa FK506-binding protein (FKBP25), previously shown to be regulated by p53-mediated repression, as...

journal_title：FEBS letters

authors： Ochocka AM,Kampanis P,Nicol S,Allende-Vega N,Cox M,Marcar L,Milne D,Fuller-Pace F,Meek D

更新日期：2009-02-18 00:00:00

abstract：:The role of low M(r) phosphotyrosine protein phosphatase (PTPase) in the control of cell proliferation was studied. A synthetic gene coding for PTPase was transfected and expressed in NIH/3T3 fibroblasts. The effects of the enzyme were particularly evident when cells were stimulated by platelet-derived growth factor (...

journal_title：FEBS letters

authors： Berti A,Rigacci S,Raugei G,Degl'Innocenti D,Ramponi G

更新日期：1994-07-25 00:00:00

abstract：:Cellular ageing is characterized by the accumulation of oxidatively modified proteins which may be due to increased protein damage and/or decreased elimination of oxidized protein. Since the proteasome is in charge of protein turnover and removal of oxidized protein, its fate during ageing and upon oxidative stress ha...

journal_title：FEBS letters

authors： Friguet B

更新日期：2006-05-22 00:00:00

abstract：:Five-lipoxygenase-activating protein (FLAP) is usually described as an essential protein to activate the leukotriene (LTs) synthesis via the 5-lipoxygenase pathway. In the enterocyte model HT29 cl.19A cell line, 5-lipoxygenase metabolism was found despite the lack of FLAP expression. Therefore HT29 cl.19A represents a...

journal_title：FEBS letters

authors： Battu S,Beneytout JL,Pairet M,Rigaud M

更新日期：1998-10-16 00:00:00

abstract：:Crystallographic analysis of a mutated form of "loopful" GH19 chitinase from rye seeds a double mutant RSC-c, in which Glu67 and Trp72 are mutated to glutamine and alanine, respectively, (RSC-c-E67Q/W72A) in complex with chitin tetrasaccharide (GlcNAc)₄ revealed that the entire substrate-binding cleft was completely o...

journal_title：FEBS letters

authors： Ohnuma T,Umemoto N,Kondo K,Numata T,Fukamizo T

更新日期：2013-08-19 00:00:00

abstract：:This study was undertaken to demonstrate the unique specificity of the chemokine receptor CXCR4 antagonist AMD3100. Calcium flux assays with selected chemokine/cell combinations, affording distinct chemokine receptor specificities, revealed no interaction of AMD3100 with any of the chemokine receptors CXCR1 through CX...

journal_title：FEBS letters

authors： Hatse S,Princen K,Bridger G,De Clercq E,Schols D

更新日期：2002-09-11 00:00:00

abstract：:Using sensitive methods of sequence analysis including hydrophobic cluster analysis, we report here a hitherto undescribed family of modules, the BAH (bromo-adjacent homology) family, which includes proteins such as eukaryotic DNA (cytosine-5) methyltransferases, the origin recognition complex 1 (Orc1) proteins, as we...

journal_title：FEBS letters

authors： Callebaut I,Courvalin JC,Mornon JP

更新日期：1999-03-05 00:00:00

abstract：:Antibodies have been prepared against the 2-oxoglutarate transport proteins purified from bovine heart and rat liver mitochondria. The anti-heart antiserum cross-reacts with the 2-oxoglutarate carrier (OGC) from beef, pig, rat and rabbit heart, but not with the OGC from liver of the same animals. Conversely, the anti-...

journal_title：FEBS letters

authors： Zara V,De Benedittis R,Ragan CI,Palmieri F

更新日期：1990-04-24 00:00:00

abstract：:We have recently cloned a cDNA encoding sucrose:fructan 6-fructosyltransferase (6-SFT), a key enzyme of fructan synthesis forming the beta-2,6 linkages typical of the grass fructans, graminans and phleins [Sprenger et al. (1995) Proc. Natl. Acad. Sci. USA 92, 11652-11656]. Here we report functional expression of 6-SFT...

journal_title：FEBS letters

authors： Sprenger N,Schellenbaum L,van Dun K,Boller T,Wiemken A

更新日期：1997-01-06 00:00:00

abstract：:Effects of amino acid substitutions in the first extracellular loop region of the delta- and mu-opioid receptors were examined. Substitution of lysine-108 of the delta-receptor (delta K108) with asparagine improved affinity to [D-Ala2,MePhe4,Gly-ol5]enk ephalin (DAGO), a mu-selective peptide agonist, to be comparable ...

journal_title：FEBS letters

authors： Fukuda K,Terasako K,Kato S,Mori K

更新日期：1995-10-09 00:00:00

abstract：:The usefulness of a gamma camera system for external imaging of the degradation sites of low-density lipoprotein (LDL) is demonstrated in rats. [131I]LDL was injected intravenously in normal rats and rats pretreated with 17 alpha-ethinyl estradiol for LDL receptor induction. Distribution of the radioligand was followe...

journal_title：FEBS letters

authors： Friedrich E,Dresel HA,Sinn H,Schettler G

更新日期：1985-05-06 00:00:00

abstract：:Agonist-induced changes in expression and mRNA levels of luteinizing hormone (LH) receptors were compared during stimulation of ovarian follicular maturation and luteinization by gonadotropic hormones. Three major species of LH receptor mRNA, 5.8, 2.6 and 2.3 kb, were present throughout differentiation and changed sim...

journal_title：FEBS letters

authors： Hu ZZ,Tsai-Morris CH,Buczko E,Dufau ML

更新日期：1990-11-12 00:00:00

abstract：:A characteristic of the antioxidant, probucol, is its inability to inhibit apolipoprotein B fragmentation in low density lipoprotein (LDL), despite a pronounced ability to inhibit lipid oxidation on relatively lengthy exposure to Cu(II). Here we show that a short exposure of LDL to hydrogen peroxide and Cu(II) leads t...

journal_title：FEBS letters

authors： Hunt JV,Bailey JR,Schultz DL,McKay AG,Mitchinson MJ

更新日期：1994-08-08 00:00:00

abstract：:To investigate interactions between the polychlorinated biphenyl-binding protein uteroglobin and phospholipids, we used a liposome-pelletting assay. PCB-BP/uteroglobin bound to liposomes made from negatively charged phospholipids (PtdSer and PtdIns) in the presence of 5 mM calcium. No binding to liposomes made from ph...

journal_title：FEBS letters

authors： Nord M,Gustafsson JA,Lund J

更新日期：1995-11-06 00:00:00

abstract：:Dried cells of Brevibacterium ammoniagenes are a good enzyme source for the preparative synthesis of CoA from pantothenic acid, L-cysteine and ATP. A problem with this synthesis is that the CoA synthesis is repressed by negative feedback inhibition by CoA to pantothenate kinase, the first step enzyme for the biosynthe...

journal_title：FEBS letters

authors： Shimizu S,Komaki R,Tani Y,Yamada H

更新日期：1983-01-24 00:00:00

abstract：:We tested the effect of melatonin on membrane fluidity in microsomes of a rat liver model in which lipid peroxidation was induced by the addition of FeCl3, ADP and NADPH. Membrane fluidity was monitored using fluorescence spectroscopy and lipid peroxidation was estimated by quantifying malonaldehyde (MDA)+4-hydroxyalk...

journal_title：FEBS letters

authors： García JJ,Reiter RJ,Guerrero JM,Escames G,Yu BP,Oh CS,Muñoz-Hoyos A

更新日期：1997-05-26 00:00:00

abstract：:Myocyte stress 1 (MS1) is a recently described striated muscle actin-binding protein that is up-regulated in the early stages of pressure overload left ventricular hypertrophy. The aim of this study was to determine whether MS1 induces cellular hypertrophy and protects against apoptosis. Over-expressed MS1 co-localize...

journal_title：FEBS letters

authors： Koekemoer AL,Chong NW,Goodall AH,Samani NJ

更新日期：2009-09-03 00:00:00

abstract：:The role of the Ca(2+)-activated tyrosine kinase, Pyk2, in the pleiotropic coupling of nerve cell stimulation to the MAP kinase cascade still remains undefined. Using a panel of PC12 clones, one of which was defective in Pyk2, we demonstrate (1) that the MAP kinase response induced by a [Ca(2+)](i) rise (following app...

journal_title：FEBS letters

authors： Barsacchi R,Heider H,Girault J,Meldolesi J

更新日期：1999-11-19 00:00:00

abstract：:Dystrophin, a component of the muscle membrane cytoskeleton, is the protein altered in Duchenne Muscular Dystrophy (DMD) and Becker Muscular Dystrophy (BMD). Dystrophin shares significant homology with other cytoskeletal proteins, such as alpha-actinin and spectrin. On the basis of its sequence similarity with alpha-a...

journal_title：FEBS letters

authors： Chan Y,Kunkel LM

更新日期：1997-06-30 00:00:00

abstract：:The von Hippel-Lindau (VHL) tumor suppressor gene regulates the extracellular matrix by controlling fibronectin deposition. To identify novel VHL target genes, we subjected mRNA from VHL-deficient RCC cells (786-0-pRC) and a transfectant re-expressing wildtype VHL (786-0-VHL) to differential expression profiling. Amon...

journal_title：FEBS letters

authors： Bluyssen HA,Lolkema MP,van Beest M,Boone M,Snijckers CM,Los M,Gebbink MF,Braam B,Holstege FC,Giles RH,Voest EE

更新日期：2004-01-02 00:00:00

abstract：:The plant toxin ricin has proven valuable as a membrane marker in studies of endocytosis as well as studies of different intracellular transport steps. The toxin, which consists of two polypeptide chains, binds by one chain (the B-chain) to both glycolipids and glycoproteins with terminal galactose at the cell surface...

journal_title：FEBS letters

authors： Sandvig K,van Deurs B

更新日期：1999-06-04 00:00:00

abstract：:Ankyrin repeat domain 17 (Ankrd17) encodes an ubiquitously expressed protein with two clusters of ankyrin repeats. We have used gene targeting strategy to ablate the Ankrd17 gene in mouse. The Ankrd17-deficient mice died between embryonic day (E) 10.5 and E11.5 due to cardiovascular defects. Serious hemorrhages were d...

journal_title：FEBS letters

authors： Hou SC,Chan LW,Chou YC,Su CY,Chen X,Shih YL,Tsai PC,Shen CK,Yan YT

更新日期：2009-09-03 00:00:00

abstract：:Azide-binding to the heme-copper binuclear center of bo-type ubiquinol oxidase from Escherichia coli was investigated with Fourier-transform infrared spectroscopy. Deconvolution analyses of infrared spectra of the azide (14N3)-inhibited air-oxidized form showed a major infrared azide antisymmetric stretching band at 2...

journal_title：FEBS letters

authors： Tsubaki M,Mogi T,Hori H

更新日期：1999-04-23 00:00:00

abstract：:The muscular content of the mRNAs encoding the five subunits of the nicotonic acetylcholine receptor was measured during postnatal development in the rat. Subunit specific mRNAs show differential regulation. The levels of the alpha-, gamma- and delta-subunit specific mRNAs decrease steadily after birth, while the beta...

journal_title：FEBS letters

authors： Witzemann V,Barg B,Criado M,Stein E,Sakmann B

更新日期：1989-01-02 00:00:00

abstract：:The MET receptor tyrosine kinase is deregulated primarily via overexpression or point mutations in various human cancers and different strategies for MET inhibition are currently evaluated in clinical trials. We observed by Western blot analysis and by Flow cytometry that MET inhibition by different MET small molecule...

journal_title：FEBS letters

authors： Leiser D,Pochon B,Blank-Liss W,Francica P,Glück AA,Aebersold DM,Zimmer Y,Medová M

更新日期：2014-03-03 00:00:00

abstract：:Replication of Brucella inside eukaryotic cells is essential for pathogenesis, and successful infection requires rapid adaptation to the intracellular milieu. Close relatives of Brucella use the two-component system FixLJ to survive inside the host. We aimed to identify a homologous sensor in Brucella abortus. A predi...

journal_title：FEBS letters

authors： Roset MS,Almirón MA

更新日期：2013-09-17 00:00:00